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Articles by Mirza Mofazzal Islam
Total Records ( 2 ) for Mirza Mofazzal Islam
  Shamsunnahar Mukta , Sumon M. Hossain , Khondoker M. Nasiruddin and Mirza Mofazzal Islam
  Background and Objective: Salinity is becoming a serious problem in the world and a widespread soil problem in rice growing countries. The saline area is 3 times larger than land used for agriculture. The conventional methods of plant selection for salt tolerance are difficult because of the large effects of the environment. The main objective of this study was to develop salt-tolerant rice varieties by identifying suitable parents and genetic diversity analysis. Materials and Methods: The study was conducted under Biotechnology Division, Bangladesh Institute of Nuclear Agriculture (BINA), Mymensingh, Bangladesh. Initially 80 germplasms were used to evaluate the salinity tolerance at seedling stage at glass-house following IRRI standard protocol. Among them, 12 were found as salt tolerant, 13 were found as moderately tolerant, 29 were highly susceptible and 26 were susceptible by phenotypic analysis. Among them, 25 germplasms were used for molecular study, which carry all tolerant variety found in phenotypic study (Hogla, Jamai Naru, Dakhsail, Patnai, Kute Patnai, Holde Gotal, Bazra Muri, Ghunshi, Tal Mugur, Nona Bokhra, Kashrail and FL378), 7 were moderately tolerant, 5 were highly susceptible and 1 was susceptible. These germplasms were characterized by 3 SSR markers which are RM510, RM585 and RM336. Data were analyzed by POPGENE (version 1.31), Power Marker (version 3.25) and NTSYS-PC (version 2.2). Results: The number of alleles/locus ranged from 10-12, with an average number of alleles of 11/locus and PIC values ranged from a low of 0.8533 (RM336) to a high of 0.8940 (RM585). The average gene diversity of overall SSR loci for the 25 genotypes was 0.8885, ranged from 0.9024-0.8672. Unweighted pair group method of arithmetic means (UPGMA) dendrogram constructed from Nei’s (1972) genetic distance produced five distinct clusters of 25 rice genotypes. FL378 of IRRI was used as check variety. It is confirmed that Holde Gotal, Bazra Muri and Hamai were salt tolerant compared to FL378. Conclusion: This scientific information could be used for solution of suitable parents, development of salt tolerant rice varieties, gene identification for salt tolerance and genetic diversity analysis.
  Salil Kumar Bhowmik , Mirza Mofazzal Islam , Reza Mohammad Emon , Shamsun Nahar Begum , Aysha Siddika and Sharmin Sultana
  Eleven genotypes, including the salt tolerant cultivar Pokkali as check, were used to evaluate salinity tolerance phenotypically and genotypically. Three selected SSR primers viz., RM7075, RM336 and RM253 were used to evaluate rice genotypes for salt tolerance. Two setups were maintained for this study viz., the seedling and reproductive stages. Phenotyping at the seedling stage was done in hydroponic system using salinized (EC 12 dS m-1) nutrient solution and at the reproductive stage using salinized tap water (EC 6 dS m-1). IRRI standard protocol was followed to evaluate salinity tolerance in rice. The genotypes having similar banding pattern with Pokkali were considered as tolerant. Phenotypically, three genotypes Pokkali, THDB and TNDB-100 and five genotypes RD-2586, TNDB-100, Dhol Kochuri, PNR-519 and Pokkali were identified as salt tolerant at the seedling and reproductive stages, respectively. These genotypes were also identified as salt tolerant genotypically (with markers). Through phenotypic and genotypic study, five genotypes viz., Pokkali, Dhol Kochuri, RD-2586, TNDB-100 and PNR-519 were identified as salt tolerant. Therefore, these microsatellite markers used in this study could be efficiently used for identification of salt tolerant rice varieties in marker-assisted breeding and quantitative trait loci analysis.
 
 
 
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