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Articles by Mingqi Liu
Total Records ( 2 ) for Mingqi Liu
  Wei Yang , Xianjun Dai and Mingqi Liu
  Listeria monocytogenes is an emerging bacterial food borne pathogen, which could survive common stress levels and often cause listeriosis. To control its infection, it is necessary to establish a sensitive, specific, rapid technology for detecting Listeria monocytogenes. In this study, invasion-associated-protein (IAP) was used as a target gene. After prokaryotic cloning and expression of the gene, the specific membrane protein P60 of Listeria moncytogenes was obtained. The P60 was injected into New Zealand white rabbits to get the antibody. The prepared antibody was conjugated with magnetic-bead to get immunomagnetic-beads. Enzyme-linked immunosorbent assays method based on immunomagetic-bead (IMB-ELISA) would be developed through immunomagetic-bead (IMB) collecting Listeria moncytogenes. The results showed that the sensitivity of the IMB-ELISA was 1.2x106 CFU mL–1, the limit of detection was 31 CFU mL–1. The IMB-ELISA is a quick and accurate method for the detection of Listeria moncytogenes.
  Jia Liu , Jiangying Hu , Mingqi Liu , Guozhou Cao , Jianguo Gao and Youfu Luo
  Background and Objective: Zinc oxide has achieved increasing attention in an extensive range of areas, due to its strong antimicrobial effect and generally recognized as safe material listed by FDA. However, the possibility of migration from commercial products is always an issue of mutual concern when its application is food packaging. This study evaluated the migration of nano-zinc oxide from polypropylene food containers to the food-simulating solutions based on the Chinese standard. Methodology: Several experimental factors influenced zinc oxide release: Food simulant, temperature and storage time. Results: Results revealed a significant nano-zinc oxide migration into oily, acidic and aqueous simulants. The amount of zinc oxide migrated increased with storage time and temperature although, zinc oxide showed a low tendency to migrate into food simulants. Conclusion: The Zn2+ substance was quantified by inductively coupled plasma mass spectroscopy (ICP-MS) and migration was found to occur within a range of 0.15-0.56 μg L–1. Meanwhile, Scanning Electron Microscopy/Energy Dispersive Spectrometer (SEM/EDS) and Malvern Zetasizer Nano were applied to identify the existence and the morphology of nano-zinc oxide.
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