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Articles by Ming-Zhou Li
Total Records ( 3 ) for Ming-Zhou Li
  An-An Jiang , Ming-Zhou Li , Kai Zhang , Yi-Ren Gu and Xue-Wei Li
  PPARGC1A, the gene encoding peroxisome proliferative activated receptor-γ coactivator-1α is a useful candidate gene for pork quality due to its regulation of the determination of myofibre type. In this study, changes in developmental gene expression of PPARGC1A in longissimus dorsi muscles were examined in two pig breeds, Landrace (lean) and Meishan pigs (obese) at birth, 30, 60, 90, 120 and 150 days of age using quantitative real-time PCR. The results indicated that there were distinct expression patterns of PPARGC1A in the two pig breeds. At most stages, except at 30 and 60 days, the abundance of PPARGC1A mRNA in Meishan pigs was significantly higher than that in Landrace (p<0.01). Interestingly, there was a negative correlation between the PPARGC1A expression pattern and myofibre cross-sectional area across five developmental stages which implies that PPARGC1A might have an important influence on myofibre growth.
  Jiu-Qiang Guan , Ming-Zhou Li , Ping-Er Lou , Su-Rong Shuai , Qing Zhao and Xue-Wei Li
  The three adiponectin-related genes (AdipoQ, AdipoR1, AdipoR2) have been notably identified in association with adiponectin levels in vivo and obesity phenotypes. The mRNA levels of AdipoQ, AdipoR1 and AdipoR2 have been measured across six different adipose tissues from the female leaner Landrace and female fatty Rongchang pig breeds using quantitative real time RT-PCR (q-PCR) approach. The mRNA levels of AdipoQ (PB = 2.27x10-9), AdipoR1 (PB = 0.04) and AdipoR2 (PB = 5.72x10-6) were higher in the leaner Landrace pigs than in the fatty Rongchang pigs. The mRNA levels of AdipoQ (PT (VATs vs. SATs) = 2.67x10-3) and AdipoR2 (PT (VATs vs. SATs) = 2.27x10-4) were higher in SATs compared with VATs. These results present the breed and tissue-specific expression patterns of AdipoQ, AdipoR1 and AdipoR2 which highlight their potential as candidate genes for the pig fat mass trait.
  Ping-Er Lou , Ying-Kai Liu , Tao Wang , Ming-Zhou Li and Xue-Wei Li
  Monozygotic (MZ) twins having the same DNA information have been used for many biomedical studies. Pigs present high similarity with humans and have been used as an ideal mammalian model the use of MZ twins is even more valuable. In the study, researchers present a systematic method for the stepwise exclusion of inconsistently genotyped individuals in a large population by Short Tandem Repeat (STR) genotyping. Five pairs of microsatellite markers with different fluorescent dyes were selected and their reliability was tested. The results demonstrate that the cumulative exclusion rate of all markers was 99.67% in 1,201 pigs; two pairs of MZ twins were initially identified. Researchers have established an effective, easy and cheap way to identify MZ twin pigs.
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