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Articles by Ming Chang
Total Records ( 2 ) for Ming Chang
  Zhi-Qiang Liu , Da-Wei Li , Zhao-Xia Zang , Ming Chang and Lin-Sen Hu
  Endothelial Progenitor Cells (EPCs) may play a crucial role in postnatal vasculogenesis which contribute to the development of vascular biology. So far, there has not a consensus definition of EPCs. In 2004, Ingram developed a single-cell clonogenic assay and identified a unique cell population based on their proliferative potential in human umbilical cord blood and adult peripheral blood and named this cell population as Endothelial Colony-Forming Cells (ECFCs). Accumulating data suggest that ECFCs possess all the characteristics of EPCs including robust clonal proliferative capacity and the ability to form de novo blood vessels with a lumen in vivo and capillary-like tube in vitro. Therefore, ECFCs may be true EPCs and have been highly concerned as a superior type of cells to treat patients with vascular diseases. This review will focus on the characteristics, hierarchy, expansion, functions of ECFCs. Researchers attempt to provide a comprehensive understanding of the cells to promote the development of cell-based therapy for patients suffering from vascular diseases.
  Jianglong Yuan , Yong Jin , Bing Zhu , Wei Wang , Yu Ren , Haiqing Wu , Fei Hao , Mingtu Nuo , Muzi Jin , Zhigang Wang , Ming Chang , Dongjun Liu and Xudong Guo
  The efficiency of donor cell types in cashmere goat Somatic Cell Nuclear Transfer (SCNT) is undefined. In this study, SCNT was performed using Caprine fetal Fibroblast Cells (CFCs), Caprine Ear Fibroblast Cells (CEFCs) and Transgenic Caprine Fetal Fibroblast Cells (TCFCs) as donor cells to compare the influences of cell type, transgene and sex of nuclear donor fibroblast cells on the SCNT efficiency including fusion and cleavage rates of reconstructed embryos and the birth, postnatal survival and mortality rates of cloned kids. A total of 4,943 reconstructed embryos were obtained. Among them, 3,949 embryos were fused and 3,737 embryos were cultured in vitro leading to a total of 3,094 cleavage embryos. Furthermore, embryo transplantation analysis was conducted on 1,873 cloned embryos with relatively normal morphology. A total of 368 recipient goats were transplanted and 48 goats were born of which 35 goats survived.
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