|
|
| |
| Articles
by
Milind Madhukar Chawak |
Total Records (
1 ) for
Milind Madhukar Chawak |
|
 |
| |
|
| |
Mohd Yaqoob Wani
,
Kuldeep Dhama
,
Shyma K. Latheef
,
Rajamani Barathidassan
,
Ruchi Tiwari
,
Sandip Chakraborty
,
Milind Madhukar Chawak
and
Shambhu Dayal Singh
|
| |
Chicken Infectious Anaemia Virus (CIAV) is one of the potent
immunosuppressive and economically important agents affecting poultry industry
worldwide. Recent reports indicate the emergence of this virus in the poultry
flocks of the country. The present study aimed to investigate the pathogenic
potential of a recent isolate of CIAV obtained from poultry flock of Uttaranchal
State, India. Twenty first day-old age Specific Pathogen Free (SPF) chicks were
inoculated intramuscularly with 104.5 median tissue culture infective
dose (TCID50) of CIAV passaged in the Marek’s
disease virus transformed chicken splenic T lymphocyte (MDCC-MSB1) cell line
while 15 chicks were kept as control. The CIAV isolate produced consistent clinical
signs, loss in body weight gain, anaemia, low haematocrit values, bone marrow
aplasia and generalized lymphoid atrophy. Mean Packed Cell Volume (PCV) value
of the infected chicks was significantly low (18.22±2.22) compared to
control group (34.12±4.72) at 14 day post infection (dpi). The establishment
of virus infection in chicks was confirmed both at molecular and antigenic levels
by Polymerase Chain Reaction (PCR) and Indirect Immunofluorescent Test (IIFT),
respectively. Characteristic apoptotic pattern was also detected in the affected
organs and the virus was re-isolated successfully in MDCC-MSB1 cell cultures.
The present results revealed that the virus circulating in poultry flocks of
Uttaranchal state is both pathogenic and immunosuppressive in nature. Extensive
epidemiological studies are suggested in the poultry flocks of the country along
with adaptation of appropriate diagnostic, prevention and control strategies
so as to prevent economic losses caused by this important poultry pathogen. |
|
|
|
|
| |
|
