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Articles by Messaouda Boukhemis
Total Records ( 2 ) for Messaouda Boukhemis
  Baida Djeghri-Hocine , Messaouda Boukhemis and Abdeltif Amrane
  Problem statement: Lactic Acid Bacteria (LAB) is characterized by fastidious nutritional requirements leading to the need for complex and rich media to allow growth. Owing to their complex requirements which also varied significantly with species, the formulation of selective media for LAB is difficult. Approach: A culture medium for LAB was previously developed based on deproteinated whey supplemented with yeast autolysate and de-lipidated egg yolk. Based on this previously developed medium, some selective media were formulated and their potential for LAB selection and enumeration was examined. Results: In this study, it was considered as a basis to define a selective medium, by adding a pH indicator and some inhibitory compounds to select the LAB flora of various dairy products including milk, disinfected or not, fermented milk and yoghurt. The addition of sodium azide to inhibit the contamination flora, including Gram negative floraand purple bromocresol allowed a direct selection of the Gram + and lactose + flora. An acidic pH (5.0) can also be helpfully considered after protein hydrolysis to avoid protein denaturation. Conclusion: To complete this study, additional work is needed concerning the improvement of culture medium selectivity by considering other inhibitors, of the Gram negative flora (like lithium chloride for instance), of fungi (like actidione), of the Gram positive flora (excepting the LAB flora) and an association of these inhibitors.
  Zohra Kassas , Baida Djeghri-Hocine , Saida Hanoune , Zineb Derradji , Aicha Boudour and Messaouda Boukhemis
  Plackett-Burman design was used to determine the most influential of 9 variables (glucose, lactulose, tween80, K2HPO4, sodium acetate, MgSO4, pH, shaking and inoculum size) on the growth of L. plantarum BH14. Glucose, lactulose, MgSO4 and shaking had a significant effect on growth of this strain at a 70% confidence level. Glucose, lactulose and MgSO4 showed positive coefficient but shaking had a negative coefficient, thus shaking was avoided in subsequent experiments. Composite design was used to determine the optimum concentrations of the significant variables. An optimized formulation of nutrition levels was suggested from the software at the following concentrations: 11.59 g L–1 glucose, 11.59 g L–1 lactulose and 0.23 g L–1 MgSO4. Growth of L plantarum was compared to that recorded on the reference medium ‘vegetal MRS’. The results showed that viable counts in the optimized culture medium were significantly higher than those in the vegetal MRS medium.
 
 
 
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