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The article presents the researches carried out with fresh royal jelly in different larvae stage. At the beginning, the initial material was analyzed on the content of nutritious substances. This type of work is extremely valuable for practical application in food science, therapy and nutritive supplements. In scientific word royal jelly has been accepted and used as nutritive substance complex, benefic for humans health. The primary objective of this study was to evaluate the physicochemical parameters of different samples of royal jelly in different larvae stage. The principal constituents of royal jelly are water, protein, sugars, lipids and mineral salts. Although they occur with notable variations, the composition of royal jelly remains relatively constant when comparing different colonies, bee races and time of harvesting.