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Articles by Mehrdad Nazeri
Total Records ( 19 ) for Mehrdad Nazeri
  Adel Feizi and Mehrdad Nazeri
  Newcastle disease is a serious and commonly fatal disease of chickens caused by a Paramyxovirus. Other avian species are also infected, but usually with less severe consequences. In most developing countries Newcastle disease is the most important infectious disease affecting village chickens. The usual source of infection for village chickens is usually other chickens. The role of other birds as carriers to initiate outbreaks in villages is not well documented. Both epidemic and endemic forms of Newcastle disease occur in village conditions. The aim of this study was to assess the effect of Newcastle disease on broiler breeder performance. In this study, four ND afflicted broiler breeder flocks were selected and the effect of this disease was appraised on these flocks. Of cases studied can be refer the mortality rate, production loss, clinical signs, necropsy signs and disease period. Also in afflicted flocks blood sampling was taken in early and 14 days after of involvement to recording the antibody titer by HI test. Of each saloon number of 16 samples was taken. Samples were referred to lab immediately. In lab, HI test was carried out and antibody titer of ND was measured. It must be noted that race of all flocks was Ross. After sampling from chicks on mentioned days were used of HI method for measurement of serum antibody titer. In this study revealed that antibody titer in before and after N.D has significant difference (p<0.05) and antibody titer was increased totally about 2 log after N.D.
  Adel Feizi and Mehrdad Nazeri
  The main objective of this study was to determine the relation between the incidence of ascites syndrome and the ventilation factor in broiler chickens in the northwest region of Iran. We studied seven flocks which showed ascites syndrome. We started to correct unsuitable ventilation conditions in all seven poultry houses. After correction of the ventilation conditions, the incidence rate of ascites syndrome decreased by 1% in all seven poultry houses and we could nearly reduce the prevalence of CRD complex from 7% to 1% in these flocks too.
  Yousef Doustar , Daryoush Mohajeri , Younes Anzabi and Mehrdad Nazeri
  Influenza virus produces cell death in animals and human. Since cell death can be caused by either necrosis or apoptosis. We investigated the types of cell death that occur in chickens infected with avian influenza virus, A/chicken/Iran/772/2000(H9N2). In experimental study 60 SPF chickens at 3 weeks old were divided to two groups. The first group was infected with 107.5 EID50 titer of the virus intranasaly and the second group was treated with saline normal. Following 72 hrs, renal tissues were collected and fixed in 10% formalin solution. The prepared microscopic sections with the thickness of 5-6 micron were stained using TUNEL method. In comparison to the control group, there were significant mean difference of apoptotic cells in renal tubular cells of the infected group (p<0.005). We demonstrated that A/chicken/Iran/772/2000 (H9N2) is able to induce apoptosis in renal tubular cells.
  Amirparviz Rezaeisaber and Mehrdad Nazeri
  Fatty liver syndrome (Hepatic lipidosis) or fat cow syndrome is a major metabolic disorder in many dairy cattle’s in early period of lactation. The aim of this study was to evaluating fatty liver syndrome in dairy cattle in Tabriz by measurement of TG, APO-A and APO-B serum values. The results showed that TG has a negative correlation with APO-A and APO-B. Thus with elevating of TG serum values, APO-A and APO-B contrary diminished.
  Adel Feizi , Farhad Dadian and Mehrdad Nazeri
  The most important genus of thyme is thymus vulgaris known as a garden herb of thyme, which its biologic and pharmacologic effects include: expectorant, antiseptic, anticough, and also anti whooping cough. The recent studies show that thyme has significant effects too, on typhoid, diphtheria, colibacillus, pneumonic bacteria, staphylococcal, streptococcal bacteria, and also has antifungal and antioxidant effects. In this research, 600 day old LOHMAN broilers in 5 groups of 30 selected as experimental and control groups; and in all of these groups vaccination process and also determination of the final weight (FCR) have been used, which in experimental group different doses of essence have been received and after vaccination the samples were taken, and study process of hystopathological changes was done. Regarding to findings and statistical studies which were done SPSS software, the trachea edema factors, tracheitis, pulmonary edema, bronchitis, have been surveyed. In the results obtained from the 6th day onwards IB vaccine (H120) of all above cases experimental group reduced by increasing dose.
  Amirparviz Rezaeisaber and Mehrdad Nazeri
  Internal parasitism of feedlot cattle has been documented to reduce performance and impair immune function. GI nematodes are chronic pervasive infections that contribute worldwide to morbidity and mortality in humans and in livestock. The objective of this study was to evaluation of Kepromec pour-on effect on decreasing of gastrointestinal nematodes. In this study, 60 male and female hybrid cows were allocated into two 30 individual groups. Then, Kepromec pour-on was administrated at the dose of 1 mg 20 kg-1. Results showed that Kepromec pour-on has about 90% efficacy on GI nematodes. Thus, use of Kepromec to prohibition of GI nematodes is recommended.
  Mohammad Rahbani , Daryoush Mohajeri , Ali Rezaie and Mehrdad Nazeri
  The negative impact of diabetes on the retinal, renal, nervous and cardiovascular systems is well recognized yet little is known about the effect of this disease on the liver. Oxidative stress is currently suggested as a mechanism underlying diabetes mellitus complications. The present study was designed to assess the liver injury as a complication of diabetes mellitus and to evaluate the hepaoprotective properties of ethanolic extract of Saffron (dried stigmas of Crocus sativus L.) in streptozotocin-induced diabetes in rats. Aminotransferases, Alanine Aminotransferase (ALT) and Aspartate Aminotransferase (AST) were measured to determine the concentration of intracellular hepatic enzymes that have leaked into the circulation and serve as a marker of hepatocyte injury. Alkaline Phosphatase (ALP) and bilirubin were measured to assess biliary function. Albumin was measured to reflect liver synthetic function. The lipid peroxidation product, Malondialdehyde (MDA) and reduced Glutathione (GSH) content was measured to assess free radical activity in the liver tissues. The enzymatic activities of Glutathione Peroxidase (GSH-Px), Superoxide Dismutase (SOD) and Catalase (CAT) were measured as indicators of antioxidation in liver tissue. Moreover, histopathological observations were assayed at the degree of hepatic injury. For this purpose, male Wistar rats were made diabetic with a single injection of STZ (75 mg kg-1 i.p.). Rats were randomly separated into four groups, each containing 10 animals: Group 1, healthy control rats; Group 2 non-diabetic rats were treated with 40 mg kg-1 b.w./day intraperitoneal (i.p.) injection of Saffron extract; Group 3, diabetic rats; Group 4, diabetic rats were treated with saffron extract (40 mg kg-1 b.w./day, i.p.) for a period of 8 weeks. At the end of the experiment, MDA contents of the liver tissue and serum levels of ALT, AST, AP and bilirubin in Groups 3 were found to be significantly increased as compared to Group 1 (p<0.05) and these serum biomarkers of hepatic injury and liver MDA level in Group 4 were significantly decreased as compared to Group 3 (p<0.05). The GSH, SOD, CAT and GSH-Px contents of the liver and serum albumin level in Group 3 was significantly decreased as compared to Group 1 (p<0.05) and were found to be significantly increased in Group 4 as compared to Groups 3 (p<0.05). Histopathological changes were in agreement with biochemical findings. Based on these findings, it is presumed that ethanolic extract of C. sativus L. stigma may have the hepatoprotective effect in experimentally induced diabetic rats. Researchers suggest that saffron extract has beneficial effects on antioxidant defence of diabetic liver tissue.
  Ali Rezaie , Arash Pezeshki , Behzad Zadfattah , Mehrdad Nazeri , Changiz Ahmadizadeh and Babak Mohammadi
  Celandine with scientific name Chelidounium majus, spreads across the world and exists in Northern parts of Iran like Gilaan, the outskirts of Rasht, Roodbar and Mazandaran. Some of effective chemical compounds are: malic, copticine, cityicacid, chelerythrine, berberine, succinic acid and sangainarine acid. The herb’s other medical properties are: calmative, narcotic, anti spasm, lowering blood pressure, antibilious, cathartic and wormroot in internal uses. Epispastics and flesh wound amneliorator in external uses. Other chemical compounds of the herb have alkaloids that some of which are toxic those alkaloids are Chelidtine hemochelidotine, cheleritrine, sangainarine and protopine. In the present study, 30 Wistar male rats of 200-230 g weight and about 3 months old were used for laboratory experiments. Animals were kept in standard condition, at 20-25°C, 70% humidity and light cycle of 12 h lighting and 12 h darkness. Standard plates were used in order to feeding by method of ad-libitum, i.e., 24 h feeding. It can be concluded, generally that based on different studies the extract of celandine may affect via effecting on benzodiazepine receivers connected to GABA receivers (considering its flavonoid content). Based on the obtained results by the present study it can be said that according to sedation process the extract dosage of 400 mg kg-1 BW among other dosages has had more meaningful results and has a better sedation, pre-anesthetic and anti-anxiety effects compared with diazepam (p<0.01).
  Ali Rezaie , Arash Pezeshki , Behzad Zadfattah , Mehrdad Nazeri , Changiz Ahmadizadeh and Babak Mohammadi
  Hempseed with scientific name Cannabis sativa, spreads across the world. Hempseed oil’s fatty acids compounds were obtained from different parts of Iran with different weather and were tested. The herb contains canabidole, canabinol and canabidiolic acid. The herb is used for preparing some sedation drugs which are used in neural stresses, anxiety, migraine, asthmatic coughs as well as local anesthesia in dentistry. Stuart points out that all parts of hempseed are used in China traditional medicine. Its seed is used as tonic, strengthening, laxative, diuretic, calmative, gastric anti parasite and narcotic. Masson and Teobold say that hempseed has a great value for tetanus treatment. Hempseed is a real calmative for stomachache and digestive disorders as well as beneficial for cancers, ulcers and rheumatic pains. In the present study, 30 Wistar male rats of 200-230 g weight and about 3 months old were used for laboratory experiments. Animals were kept in standard condition, at 20-25°C, 70% humidity and light cycle of 12 h lighting and 12 h darkness. Standard plates were used in order to feeding by method of ad libitum, i.e., 24 h feeding. Especial dishes were used for water. The rats were numbered in groups consisted of 5 animals and were placed in especial cages.
  Adel Feizi and Mehrdad Nazeri
  Salmonella is an important cause of human disease with an estimated 80.3 million annual foodborne cases and significant economical losses in the poultry sector worldwide. The aim of this study was to evaluation prevalence of Salmonella infections in layer flocks in East Azerbaijan province of Iran. For isolation of agent after autopsy of losses, liver surface was cauterized and swap from this area was obtained and added into the tetrathionate culture media and incubated at 37°C for 15 h. Then agents obtained from previous stage were cultured in the macconkey agar culture media as five regional method and then incubated at 37°C. After several times, lightly yellow colonies appeared that suggestive non-fermentative lactose bacteria. For differentiation of Salmonella from other non-fermentative lactose bacteria, specific Medias such as TST, urea, MR-VP, SIM, simmon citrate, lysine and Medias contain sucrose, lactose, maltose, manoz and arabinose used. Finally, the amounts of losses since week 31 until 67 was 5030 hens that by adding losses before week 31 which was about 1450, the sum of losses was 6480 or 27% of flock. This study revealed that contamination rate in East Azerbaijan province of Iran is higher than standard levels and because of zoonotical issues between poultries and human must be take measures in this field.
  Amirparviz Rezaeisaber , Reza Zarei and Mehrdad Nazeri
  Zinc is an essential mineral that is naturally present in some foods, added to others and available as a dietary supplement. Zinc is also found in many cold lozenges and some over-the-counter drugs sold as cold remedies. Zinc is involved in numerous aspects of cellular metabolism. It is required for the catalytic activity of approximately 100 enzymes and it plays a role in immune function, protein synthesis, wound healing, DNA synthesis and cell division. Zinc also supports normal growth and development during pregnancy, childhood and adolescence and is required for proper sense of taste and smell. A daily intake of zinc is required to maintain a steady state because the body has no specialized zinc storage system. The aim of this study was to measurement of zinc in Arabian horse’s serum by Spectrophotometric Method in Tabriz. In this study about 186 blood samples from apparently healthy Arabian horses were obtained and blood samples taken near the ice and sent to the laboratory and after serum preparation were freezing inside the micro tube. In lab, blood samples centrifuged at 2000 rpm for 15 min.
  Daryoush Mohajeri and Mehrdad Nazeri
  Non-Alcoholic Fatty Liver Disease (NAFLD) is now recognized as the most common type of liver disease and might lead to an important public health problem. The aim of the present study was to evaluate the protective effects of Crocin, a unique water-soluble glycosylated carotenoid in the stigmas of the Crocus sativus Linne and in the fruits of Gardenia jasminoides Ellis, on rat high fat diet-induced hepatic steatosis model. Male Wistar rats were given either control diet, high fat diet alone or high fat diet plus Crocin via gavage at different doses (25, 50 and 100 mg/kg/day) for 4 weeks in different experimental groups. The serum levels of Triglyceride (TG), Total Cholesterol (TC), Low-Density Lipoprotein (LDL-C), Very Low-Density Lipoprotein (VLDL-C) and High-Density Lipoprotein (HDL-C) were detected to determine deleterious metabolic effects. Aminotransferases, Alanine Aminotransferase (ALT) and Aspartate Aminotransferase (AST) were measured to determine hepatocyte injury. Alkaline Phosphatase (ALP) and bilirubin were measured to assess biliary function. Albumin was measured to reflect liver synthetic function. The lipid peroxidation product, Malondialdehyde (MDA) and reduced Glutathione (GSH) content was measured to assess free radical activity in the liver tissues. The enzymatic activities of Glutathione Peroxidase (GSH-Px), Superoxide Dismutase (SOD) and Catalase (CAT) were measured as indicators of antioxidation in liver tissue. Finally, the biochemical findings were matched with histopathological verifications. After 4 weeks, high fat diet caused deleterious metabolic effects including hypertriglyceridemia, hypercholesterolemia and liver dysfunction. Rats fed high fat diet alone showed increased activities of hepatocellular enzymes in plasma, significant decline in antioxidants and elevated lipid peroxidation indices in liver. Crocin treatment significantly reduced elevated markers of liver tissue injury and lipid peroxidation product (MDA) and brought back the liver antioxidants and the over accumulation lipids in serum towards normal. Histopathology of the liver confirmed the changes induced by high fat diet and the heptoprotective effect of Crocin. Crocin exerts protective effects against hepatic steatosis in rats fed with high fat diet possibly through its antioxidant actions.
  Ali Rezaie , Amirreza Ebadi , Mehrdad Nazeri , Samad Rezaie and Fatemeh Elmi
  Aloe vera is a perennial succulent belong to the Lily (Liliaceae) family. This plant has been known as the healing plant. Aloe vera has been used for traditional medical purposes in several cultures for millennia. It has been demonstrated that Aloe vera has growth promoting activities. The objective of this study was to determination of the effect of Aloe vera on healing of the experimental skin wounds on rats and its comparison with zinc oxide. Zinc oxide is being used worldwide as an absorbent and protective compound. Its pharmacological properties are wide and its non-toxic material allows it to be used as a routine skin care substance. In current study, 70 female wistar rats where included in 5 groups. Full thickness incisional wound with 23 mm diameter was made with surgical scissors and scalpel. The whole operation was taking place under general anesthesia and analgesia circumstances. After making surgical wounds, rats are treated as mentioned in the text. Rats are observed for 21 days for wound closure process and inflammatory conditions taking place in wound. Biopsy intervals are 0 (the day of surgery), 3, 7, 14 and 21th day after surgery. In these certain days rats were euthanized and biopsies of wound sites were obtained. Wounds areas are also, measured by Scion Image ™ Software daily. At last, all data were analyzed using SPSS Statistics Ver.17. As a result, Aloe vera at the dose of 10% has significant healing properties compared to Zinc oxide. These data were validating under confidence surface of 95% (p<0.01).
  Ramin Shishehgar , Ali Rezaie and Mehrdad Nazeri
  Humulus lupulus (hops) is a climbing perennial vine that vigorously grows 20-35 feet each year. Humulus lupulus is a member of the hemp family which has grown wild since ancient times in Europe, Asia and North America. The female flowers mature in late Summer and are used to add bitterness, flavor and aroma to beer. In ancient times the young shoots were eaten as a vegetable and the dried flowers were used for their slight narcotic effect and sedative action in the treatment of mania, toothache, earache and neuralgia. One modern herbal medicine practitioners continue to use hops as a sedative and mild hypnotic as well as for its endocrine, free radical scavenging and antitumor properties. The aim of this study was to investigation of the sedation, pre-anesthetic and anti-anxiety effects of hop (Humulus lupulus L.) extract compared with diazepam in rats. In the present study 30 wistar male rats weighting 300±10 g and about 3 months old were used for laboratory experiments. In order to evaluate the sedation and pre-anesthetic effects of hop extract compared with diazepam, 50 mg kg-1 of extract in 1st group, 100 mg kg-1 in 2nd group, 1.2 mg kg-1 in 3rd group, 2 mg kg-1 in Group 4th, 2 mg kg-1 amount of dimethyl sulfoxide was injected intra peritoneal in 5th group and 6th group did not receive any drug. Data showed that hop extract has better sedation, pre-anesthetic and anti-anxiety effects than diazepam. Researchers suggest that still need more studies on this plant component in order to understand the more sedative and anxiolytic effects of this plant.
  Adel Feizi and Mehrdad Nazeri
  Gout syndrome is one of the common mortality causes accounted for poultry farms. Gout is diagnosed by deposition of urates in kidney, on heart serous surfaces, liver, mesenteric, air sac and peritoneum, etc. There are two types of diseases: visceral and joint gout. Incidence of gout syndrome in broiler chicks increases mortality and decreases performance. Gout causing factors could be classified in three sections; management and nutritional factors, infectious factors and toxic factors. The current study conducted to evaluate effect of dietary calcium percentage on incidence of gout syndrome and its effect on serum biochemical parameters in broiler chicks. Total 300 broiler chicks (Ross 308), divided in two groups; treatment and control. Each group has three replicates (50). All management factors including: light (intense and duration), temperature, vaccination program, ventilation rate, nutrition, stocking density for both groups were same but dietary calcium percentage was different. Dietary calcium for treatment group was 2 and 1% in control group. Blood samples of each group were collected on 5th, 35th and 42nd days and their serum separated by centrifuging followed by biochemical parameters were measured. Serum biochemical parameters are uric acid, urea, total protein, albumin, creatinine, calcium, phosphorus, magnesium, alkaline phosphatase. In addition, macroscopic examination of renal necropsy, diet exchange ratio, mortality percentage and their relationship were evaluated in this study. Results showed that in treatment group the rate of uric acid, urea, total protein, albumin, creatinine, calcium, calcium and alkaline phosphatase have meaningful changes compared control with group (p<0.05). Evaluation of the serum level phosphorus in treatment group did reveal statistically significant decrease compared with control group (p<0.05). There was no statistical meaningful change in serum level magnesium between two groups (p>0.05). The FCR and mortality rate of treatment groups was higher than the control group. Deposition of urates in kidney, on heart serous surfaces, liver, mesenteric, air sac and peritoneum were displayed in treatment group. The urinary system of the treatment birds, in general, displayed inflammatory lesions showing abnormalities of color, size, shape and texture of kidneys and ureters. The ureters also showed occlusion and distention.
  Ali Rezaie , Babak Lotfollahi , Daryoush Mohajeri , Ghafour Mousavi , Mehrdad Nazeri , Solmaz Zakhireh and Babak Mohammadi
  Acceleration in healing of surgical skin wounds, in order to reduction of post-operation consequences is most important. One of the most popular compounds used ever for skin care is lavender oil or Lavandula stoechas essential oil. The aim of this study was evaluate the healing effects of Lavandula stoechas extract in comparison with zinc oxide ointment on full thickness experimental open surgical skin wound healing in the rats. For this purpose, 75 female Wistar rats were randomly assigned in 5 groups of 15 animals each including: Control, treated by Eucerin as the placebo, treated by zinc oxide topical ointment and two remaining as test groups which received 10 and 20% Lavandula stoechas extracts. Under the general anesthesia and analgesia circumstances, a full thickness incisional open wound with 23 mm diameter was made on the skin of the back part of the rats. The healing process was studied macroscopically and microscopically at 0 (the day of surgery) 3, 7, 14, 21 and 28th days of experiment in all of the rats. The greatest degree of wound contraction was considered in Lavandula stoechas extract (10%) treated rats while this change was slighter in control group. In microscopic examination, the overall healing process of Lavandula stoechas extract (10%) treated rats was significantly better than the other experimental groups (p<0.05). The results obtained showed that Lavandula stoechas extract (10%) has more effective healing properties on full thickness open skin wounds compared to zinc oxide topical ointment.
  Amir Reza Ebadi , Ali Rezaie , Mehrdad Nazeri and Behboud Jafari
  Allium genus has some valuable species such as onion and garlic for human being. Allium jesdianum Bois species is a bulbar and durable plant with 2-3 leaves which grows in altitudes of Arasbaran region. The herb has various uses in foods and is used traditionally in treatment and decreasing rheumatic and digestive pains as well as renal calculus excrete. The aim of this study was to evaluating the anti bacterial effects of Allium jesdianum Boiss extract. Microorganisms were cultured in agar mauler Hilton media such that swabs smeared with bacterial suspension were moved traversal on the media surface then the media was turned at angle of 60° and culturing were repeated 2 times. These results demonstrated that sulfide compounds form an important part of extract’s constituents. Also, curzerenon and curzernne are the materials which are available significantly in this extract. Anti microbial effects of understudied herb are probably due to presence of sulfide and trepnoid compounds.
  Ali Rezaie , Behboud Jafari , Ghafour Mousavi , Mehrdad Nazeri , Amirreza Ebadi , Changiz Ahmadeh and Elmira Habibi
  In the present study, sedative and anti-anxiety drugs such as chemical drugs are used for the sedation and anti-anxiety but due to their side effects and economical issues, the significance of research on finding sedative and anti-anxiety drugs with less side effects and their ability to substitute these synthetic drugs and substituting newer sedation and anti-anxiety compounds is obvious. Origanum majorana is one of the plants that have the effect sedation and anti-anxiety. The aim of this research was to examine the effects of sedative, pre-anesthetic and anti-anxiety of Origanum majorana with diazepam in rats to different groups of male Wistar rats received herbal extract Origanum majorana with doses (100, 200, 400 mg kg-1, IP) and diazepam with dose of (1/2 mg kg-1, IP) and dimethyl sulfoxide with the equal volume. About 30 min after assessing the relift/sleep inducing effect (induced sleep duration by ketamine 40 mg kg-1, IP) anti-anxiety effects (using elevated plus maze). The results show meaningful increase in the period of the sleep that had been made with ketamine and also meaningful increase in the spend time at open arms in the patient group with the previous. The results show that the dose of extract Origanum majorana 200 mg kg-1 relieving effects of sedative, pre-anesthetic and anti-anxiety and before.
  Daryoush Mohajeri , Ghafour Mousavi and Mehrdad Nazeri
  The thyroid gland in most animal species has two lobes, one on each lateral surface of the trachea. In pigs, the main lobe of the thyroid gland is on the midline in the ventral cervical region with dorsolateral projections from each side. The thyroid structural changes in gouts were investigated. About 386 pairs of thyroid glands collected from gout at the local municipal abattoir in Tabriz city of East Azerbaijan province. A total of 386 thyroid specimens examined, 8 cases were follicular atrophy, 4 cases were thyroid fibrosis, 71 cases were diffuse hyperplasia of thyroid follicular cells, 5 cases were colloid goiter, 10 cases were parenchymal cysts, 3 cases were nodular hyperplasia, 1 case was C-cell adenoma and 1 case was C-cell carcinoma.
 
 
 
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