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Articles by Mehmet TANYUKSEL
Total Records ( 3 ) for Mehmet TANYUKSEL
  Mehmet TANYUKSEL , Mustafa ULUKANLIGIL , Hasan YILMAZ , Zeynep GUCLU , R. Engin ARAZ , Gurkan MERT , Ozgur KORU and William A. PETRI, Jr

Aim: The spectrum of clinical intestinal disease due to Entamoeba histolyticainfection ranges from an asymptomatic carrier state to fulminant colitis with an array of manifestations. The extent of strain diversity for E. histolytica and the extent to which strains differ in virulence are not clearly known. In this study, we aimed to investigate the genetic diversity of E. histolytica isolates from Turkey.

Materials and Methods: In order to study genetic diversity, the serine-rich gene of E. histolytica (SREHP) was investigated in 26 clinical isolates from Turkey by nested PCR amplification and restriction enzyme fragment length polymorphism (RFLP) analysis.

Results: Twelve distinct DNA patterns were observed after AluI digestion of nested PCR products. The results demonstrate an extensive genetic variability among Turkish E. histolyticaclinical isolates.

Conclusions: Genotyping seems to be a useful epidemiologic tool to identify common-source outbreaks as well as strain-specific manifestation of an infection.

  Ozgur KORU , Ferit AVCU , Mehmet TANYUKSEL , Ali Ugur URAL , Remzi Engin ARAZ and Kenan SENER
  Aim: Caffeic acid phenethyl ester (CAPE) has cytotoxic, apoptotic, and antiproliferative effects on various tumor cells, and is the most active component of propolis. This study aimed to examine the in vitro effects of CAPE on the human multiple myeloma cell line.
Materials and methods: CAPE was added to the ARH-77 multiple myeloma cell line and the percentage of dead cells was measured using the 3-(4,5-dimethyl-thiazoyl)-2,5-diphenyl-SH-tetrazolium bromide (MTT) assay. The percentage of live cells and growth inhibition were determined using the Trypan blue test. The percentage of IL-6 cells was determined using ELISA.
Results: ARH-77 cells treated with CAPE for 72 h at the 100 μg mL-1 concentration resulted in a growth inhibition effect of 90.4% and a cytotoxic effect of 80.4%. CAPE induced apoptosis in 92.3% of the cells in 22.5 μg mL-1 at 72 h. CAPE inhibited the secretion of IL-6 by ARH-77 multiple myeloma cells at LD50 concentrations.
Conclusion: CAPE inhibited growth and secretion of IL-6, and induced apoptosis in a dose-dependent and time-dependent manner in ARH-77 multiple myeloma cells. We think that CAPE merits further study as an effective agent against multiple myeloma.
  Mehmet Tanyuksel , Ozcan Uzun , Engin Araz , Ozgur Koru and Cahit Babur
  To determine the possible relationship between toxoplasmosis and patients with first-episode schizophrenia (FES). Materials and methods: Seventy-three subjects with FES (15-54 years old; mean: 23.4 years) and 40 healthy individuals (20-54 years old; mean: 30.3 years) were enrolled in the study. Most of the FES patients (90.4%) and the control individuals (95.0%) were male. Specific IgG and IgM antibodies to Toxoplasma gondii were investigated by enzyme-linked immunosorbent assay (ELISA), and the total amount of antibodies was investigated with the Sabin-Feldman dye test (SFDT). Results: Compared to the control individuals, study subjects had significantly more toxoplasmosis-like symptoms and more cats in the household; they were less likely to live in apartments and more likely to live in ground-floor houses. They did consume more uncooked meat, unpasteurized goat’s milk, and chicken eggs; had more contact with soil; and were living less often in urban areas and more often in rural areas. Serum samples from 32 (43.8%) and 25 (34.2%) of 73 patients with FES were seropositive for T. gondii when tested by ELISA IgG and SFDT, respectively. Out of 40 serum samples from control subjects, 13 (32.5%) and 15 (37.5%) were found positive for T. gondii by ELISA IgG and SFDT, respectively. In patients with FES, 17 (68%) out of 25 who were SFDT-positive were also positive by ELISA IgG test, while 15 out of 48 (31.3%) SFDT-negative serums were positive by ELISA IgG. In the control group, 11 out of 15 SFDT-positive serum samples (73.3%) were also positive by ELISA IgG test, while 2 out of 25 SFDT-negative serum samples were positive by ELISA IgG.
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