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Articles by Meera Parthasarathy
Total Records ( 3 ) for Meera Parthasarathy
  Lourdusamy Arul Antony Maria , Vidya N. Chamundeswari and Meera Parthasarathy
  Electrophoretic separation of membrane proteins is limited mainly by their intrinsic hydrophobicity and poor solubility arising from association with membrane lipid components. In this investigation, polyacrylamide gel matrix is made hydrophobic by introducing one-dimensional and two-dimensional carbon nanomaterials viz., multi-walled carbon nanotubes and graphene. The nanocomposite matrices were optimized for SDS-PAGE with water-soluble molecular weight marker proteins. SDS-PAGE in the nanocomposite matrices of a commercial outer membrane porin protein, OmpA indicated a significant decrease in anomalous migration of the protein with increasing carbon nanotube loading in the gel matrix. Outer membrane proteins of Escherichia coli, were isolated and characterized by Fourier Transform Infrared Spectroscopy. When the nanocomposite gels were tested for electrophoretic separation of outer membrane proteins isolated from E. coli, the resolution of protein bands improved with respect to the pristine polyacrylamide gel. Especially, graphene/polyacrylamide composite hydrogels yielded far better resolution and faster migration of the E. coli membrane proteins compared to multi-walled carbon nanotube/polyacrylamide composite hydrogels. Based on contact angle measurements of the composite hydrogels, the improved resolution of membrane proteins is attributed to the more hydrophobic environment rendered by carbon nanotubes and graphene. The present results could be useful to develop more hydrophobic nanocomposite gels exclusively for electrophoretic separation of membrane proteins isolated from different sources.
  Bhuvaneshwari Veerapandian , Akshaiya Devanathan , Vivek Ogirala , Subhashree Chandramouli , Siddarth Narasimhan , Anantha Narayanan and Meera Parthasarathy
  Microbial fuel cells provide an economically and practically viable option for green generation of electric power from organic pollutants present in waste water with the help of micro-organisms. Several attempts are being made to commercialize the system for stable power generation. For portable applications, the size of the fuel cell and its volumetric power density are major determinants. In this context, microbial fuel cells with a solid electrolyte sandwiched between a cathode and anode would be an attractive configuration. In this work, we report the synthesis and characterization of highly cross-linked multi-walled carbon nanotube/polyacrylamide composite hydrogels as solid electrolyte for application in such devices. The composite hydrogels were synthesized by in situ free radical polymerization of acrylamide in presence of surfactant-dispersed multi-walled carbon nanotubes. Ionic conductivity was introduced to the hydrogels by partial hydrolysis in alkaline medium and the conductivity was compared using Electrochemical Impedance Spectroscopy. The composite hydrogels with different loadings of carbon nanotubes were characterized systematically by Differential Scanning Calorimetry, Scanning Electron Microscopy and Swelling studies.
  Viveka Kalidasan , Sruthi Sreekumar and Meera Parthasarathy
  Carbon nanotube composite hydrogels are interesting candidates for applications like flexible conductors, actuators and artificial muscles owing to their favorable characteristics such as ease of functionalization, propensity to modulate the interfacial characteristics of the hydrogels and tunable electrical conductivity. Here we report the preparation, characterization and electromechanical actuation properties of Multi-walled Carbon Nanotube/polyacrylamide (MWNT/PAM) composite hydrogels. Various amounts of MWNTs (0.002, 0.005 and 0.01 wt%) have been introduced into polyarylamide (PAM) gel matrix by in situ polymerization of acrylamide/bisacrylamide mixture in presence of MWNTs. The effect of various parameters such as nanotube content, method of drying (vacuum drying and freeze drying) and partial hydrolysis of the composite hydrogels have been investigated by following water-uptake and electro-response behavior, scanning electron microscopy and contact angle measurements.
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