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Articles by Meena Kataria
Total Records ( 2 ) for Meena Kataria
  Shanker K. Singh , Umesh Dimri , Meena Kataria and Priyambada Kumari
  Paraquat (PQ), an oxidative stress inducing substance, is a herbicide which is very toxic to the animals and humans. A number of antioxidant treatments against PQ-induced oxidative stress have been tried but unfortunately, none of them has been proven to be enormously effective. In this context, attentions are needed to explore the antioxidants from the natural resources. Thus, the antioxidant activity of Withania somnifera (Ws) root extract on paraquat (PQ) induced oxidative stress in mice was investigated. The doses of 100, 250 and 500 mg kg-1 of Ws were given orally on alternate day for 30 days. PQ (3.5 mg kg-1) was injected intraperitoneally on day 15th of start of the study. On day 30th antioxidant activity of Ws was evaluated by measuring lipid peroxides (MDA) and GSH contents as well as by measuring the activities of antioxidant enzymes; superoxide dismutase (SOD) and catalase (CAT) in the blood of mice. Aspartate aminotransferase (AST), alanine aminotransferase (ALT), total bilirubin, creatinine and urea levels were also estimated to investigate the protective effects of Ws on liver and kidney functions. Ws remarkably alleviated the over production of MDA and improved GSH level in PQ intoxicated mice blood dose-dependently. In addition, SOD and CAT activities were improved significantly by Ws treatment. Ws had also considerably alleviated the elevated AST and ALT activities as well as the total bilirubin, creatinine and urea levels in a dose dependent manner. Ws protected the mice against paraquat-induced oxidative stress mainly through alleviating the over production of MDA and modulating the lowered GSH level, SOD and CAT activities. Thus, Ws may constitute an alternative sole or adjunct remedy against paraquat-induced oxidative stress.
  Anburaja MAHALINGAM , Naveen KUMAR , Swapan K. MAITI , Ashok K. SHARMA , Umesh DIMRI and Meena KATARIA
  Ten clinically healthy, adult male dogs randomly equally divided into 2 groups (I and II) were subjected to laparoscopic sterilization and open method castration under xylazine-ketamine anesthesia. In group I laparoscopic vasectomy by cauterization and cutting of the vas deferens was performed and in group II conventional open castration by the pre-scrotal approach was done. Insufflation of the abdominal cavity was achieved with CO2 (2 L/min) at 10 mmHg pressure gradient. Two ports were needed to carry out the operation. Clinical observations revealed no significant changes. Differential leukocyte count (DLC) revealed significant neutrophilia and comparative lymphopenia on the 3rd postoperative day in both the groups. A significant increase (P < 0.05) in plasma alkaline and acid phosphatase level was observed in both the groups on day 3 postoperatively. Indices of oxidative stress viz. lipid peroxidation (LPO), catalase (CAT), superoxide dismutase (SOD), reduced glutathione activity and acute phase protein, and ceruloplasmin level in plasma did not reveal any major significant changes. Plasma cortisol level did not show any significant change in group I whereas in group II the level increased significantly (P < 0.01) after the operation. No significant change in testosterone level was observed in group I whereas in group II a significant decrease (P < 0.01) was observed immediately after the operation. On the basis of the parameters studied it can be concluded that early healing and better cosmoses were achieved by laparoscopic sterilization (vasectomy) in male dogs as compared to the conventional open method of castration and the technique can be successfully applied for mass sterilization programs.
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