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Articles by Medhat Haroun
Total Records ( 2 ) for Medhat Haroun
  Medhat Haroun
  The aim of this study was to examine the difference in serum immunoglobulin concentrations in the sera of rheumatoid arthritis (RA) patients, RA patients with acute myeloid leukaemia (AML) and to compare with those found in normal subjects. This study was performed in 43 of normal adult individuals (group 1), 40 RA patients (group 2) and 37 RA individuals with AML (group 3) were used for analysis of immunoglobulin levels by SDS polyacrylamide gel electrophoresis and ELISA. Measurements were made before treatment in the group with AML as well as when patients were both on and off chemotherapy. It was found that group 1 had a mean IgA level of 3.0 mg mL-1 and IgM level of 1.82 mg mL-1. Group 2 had a mean IgA level of 5.5 mg mL-1 and IgM level of 1.91 mg mL-1, while group 3 had a mean IgA level of 1.2 mg mL-1 and IgM level of 0.95 mg mL-1. Significant increases in IgA level in group 2 (p<0.00001) while the levels of both IgA and IgM in group 3 were significantly decreased (p<0.00001). This decrease was significant during induction and maintenance treatment, with restoration to normal once the patient was off therapy. There was no significant difference in IgG level in both groups 2 and 3 compared to group 1 (p<0.53). The finding of normal IgG level at diagnosis suggests that the leukaemia process itself did not initiate the fall. In addition, the influence course of treatment on rheumatoid arthritis patients with AML has a direct relation to the level of IgA and IgM due to complex interactions of chemotherapy.
  Medhat Haroun
  The aim of this study was to investigate the presence of anti-immunoglobulin antibodies in patients with rheumatoid arthritis, using as a target goat immunoglobulins, in an attempt to elucidate further complex immuno-pathogenetic interactions of the disease. Serum immunoglobulin A concentrations were measured by ELISA technique in twenty patients with rheumatoid arthritis and twenty matched normal adult individuals. The effect of anti-goat immunoglobulin antibodies leads to an overestimation of serum IgA level in patients; this increase is different for each sample estimated by ELISA. Visualization of IgA by immunoblotting technique confirms that goat immunoglobulins were co-precipitated with the antibody-antigen complex. Initial results before purification from the interfering anti-goat immunoglobulin antibodies suggested that patients had increment levels of IgA in their sera. It was found that normal individuals had a mean IgA level of 2.77 mg mL-1 and patients had a mean IgA level of 5.13 mg mL-1 (p<0.00004). While, the mean level of IgA in patients after purification from anti-goat immunoglobulins was 2.92 mg mL-1. Therefore, there was no significant difference in IgA level in patients purified from anti-goat immunoglobulin antibodies compared to normal individuals (p<0.33). A circulating immunoglobulin reacting with other immunoglobulins is thus present in patients with rheumatoid arthritis and may well play a part in the complex immuno pathogenetic interactions.
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