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Articles by Md. Saddam Hussain
Total Records ( 2 ) for Md. Saddam Hussain
  Md. Ibrahim , Md. Saddam Hussain , Farhana Sultana , Nazmul Abhi , Tanvir Ahmed , S.M. Naim Uddin , Atikur Rahaman , Maria Azmerin , Tutun Das Aka , Sakib Mosharraf and A.H.M. Mazbah Uddin
  Background and Objective: Although some investigations have previously been reported with this plant, the seed of the plant was not studied yet to date. This present study aimed to investigate in vivo and in vitro bioactivities of Diospyros blancoi. Cold methanolic extract of D. blancoi has been considered for evaluation of therapeutic activities such as analgesic, thrombolytic activity and anti-inflammatory. Materials and Methods: Analgesic activity was tested by formalin induced licking and biting in mice, thrombolytic activity was carried out by using human blood sample and the anti-inflammatory effect was tested by carrageenan induced paw edema method. The seeds of D. blancoi was extracted with methanol. Results: The extracts were used for the observation of analgesic activity, thrombolytic activity and anti-inflammatory activity. The D. blancoi had showed dose dependent significant analgesic activity in swiss albino mice in formalin induced method. They produced a protection of 55.67 and 73.47% from extract 100, 200 mg kg–1, respectively. In thrombolytic activity the extract shown 38.17% of clot lysis from 10 mg mL–1, in anti-inflammatory test they produced an inhibition of 24.81 and 27.23% at last hour. from extract (200 and 400 mg kg–1), respectively. Conclusion: The results demonstrated that the methanol extract of D. blancoi seed had the moderate analgesic, anti- inflammatory and thrombolytic properties.
  Afroza Sultana , Otun Saha , Ashfaqur Rahman Siddiqui , Apurbha Saha , Md. Saddam Hussain and Tarequl Islam
  Background and Objective: The increasing trend of Hospital acquired infections (HAIs), especially the ones caused by the multidrug resistant organisms has become a major public health concern. So that the aim of this study was to detect the dissemination of multidrug-resistant pathogenic bacteria on hands, gloves and masks of healthcare workers in some hospitals and pathology laboratories located in Noakhali and Dhaka, Bangladesh. Materials and Methods: A total of 106 fully fresh samples were collected. The samples were then subjected to various phenotypic cultural, biochemicals, antibiotic sensitivity along with molecular Polymerase Chain Reaction (PCR) analysis according to the standard procedures. Results: Approximately, 138 (57.5%) representative bacterial isolates were recovered among which the most frequently identified bacterium was E. coli 72 (52.17%) followed by Staphylococcus aureus 42 (30.43%), Salmonella typhi 14 (10.14%) and Pseudomonas aeruginosa 10 (7.25%). Among them total 33.33, 100 and 40% of Staphylococcus aureus, Salmonella typhi and Pseudomonas aeruginosa were pathogenic scanned through hlg, invA and OprI genes presence respectively. In contrast E. coli was tested through Congo red binding test where 36.11% were found pathogenic. The general frequency of Multiple Drug Resistance (MDR) pathogens were 79.31, 51.72, 68.97, 24.13, 6.89, 6.89, 17.24, 82.87, 24.13, 65.52 and 100% against ampicillin, chloramphenicol, ciprofloxacin, erythromycin, gentamicin, methicillin, streptomycin, tetracycline, nalidixic acid, doxycycline and imipenem consecutively. Conclusion: Healthcare personnel convey multiple drug resistance pathogenic bacteria in their protective materials which are potential source of nosocomial infections. Appropriate infection prevention measures, such as good hygiene practices and training for the healthcare workers should be taken to minimize the risks that are associated with the high rate of cross-contamination.
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