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Articles by Md. Raihan Ali
Total Records ( 2 ) for Md. Raihan Ali
  S.M. Abdul-Awal , Md. Jahangir Alam , Md. Raihan Ali and Md. Nabiul Hasan
  In vitro propagation of Trichosanthes dioica Roxb. locally known as patal has been studied. Shoot tips were cultured aseptically on MS media supplemented with different concentrations of cytokinins (BA and 2-ip) and their combinations with NAA, GA3 and CW. The effectiveness of BA was proved to be superior to that of 2-ip alone with respect to shoot proliferation. Combinations of 2-ip with CW and GA3 showed better response than 2-ip alone. The frequency of multiple shoot formation was 100% for shoot tips in MS medium containing BA 1.0 mg L-1 and BA 1.0 mg L-1 + NAA 0.2 mg L-1. A best result with BA was found when explants were cultured on MS medium supplemented with 1.0 mg L-1 BA where the average length was 15.90±0.90 mm. In combinations the highest length was 18.25±0.42 mm at 1.0 mg L-1 2-ip +0.5 mg L-1 GA3 and the highest number of shoots was 3.25±0.22 at 1.0 mg L-1 2-ip+ 10% CW. Efficient rooting was achieved on MS+IBA 0.5 mg L-1. In vitro raised plantlets were then transferred to potted soil.
  Md. Anwar Hossain , Md. Taslim Hossain , Md. Raihan Ali and S.M. Mahbubur Rahman
  An investigation was undertaken at Plant Biotechnology Laboratory, Biotechnology Discipline, Khulna University, Khulna to study the performance of different carbon sources and growth regulators on in vitro plantlet formation of Thankuni (Centella asiatica L.) from nodal segment. Among the carbon sources, sucrose showed the best results on shoot formation in presence of BA or Kn. In these cases the sucrose along with BA obtained 100% and sucrose along with Kinetin obtained 90% shoot formation, respectively. Maximum shoot length was obtained (2.67±0.059 cm) in sucrose containing BA medium. Other carbon sources showed less performance than sucrose both on BA and Kn containing media. Interestingly maltose showed the highest number of shoot/explant (4.0±0.0017) in presence of BA. In combination of BA+Kn, the percentage of shoot formation was higher (90%) in presence of sucrose, but the number of shoot/explant was maximum (3±0.035) in presence of maltose. In root formation, sucrose was found superior than other carbon sources used in present experiment. In this case IBA obtained the highest percentage of root (80%) with maximum length (1.5±0.019 cm), whereas, gur and maltose did not produce any root.

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