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Articles by Md. Monjur Hossain
Total Records ( 2 ) for Md. Monjur Hossain
  Md. Mer Mosharraf Hossain , Md. Imtiaz Uddin , Md. Ariful Haque Rupom , Jannatul Ferdoush , Md. Monjur Hossain , Subrata Mondal and Md. Anisur Rahman
  Background and Objectives: To improve the sustainable management and control of North American, western mosquitofish (Gambusia affinis) in the freshwater of Bangladesh, the most invasive to indigenous species, caused the severe infestation to all carps, genetic diversity of the species were studied using DNA sequences of nucleotides first time in Bangladesh. This study was aimed to develop molecular detection method to confirm the G. affinis in freshwater and construct a genetic baseline to control this species. Materials and Methods: This study consisted of 6 microsatellites nuclear DNA loci that were used for the construction of a genetic baseline used a gene-specific marker (cytb). A total of 6 fish for 6 sites were used for DNA extraction, then genotyped and analyzed to examine genetic diversity to assess the persistence of a species in the environment. Results: Genetic diversity was inferred as some polymorphism and monomorphism with minimal genetic distance, in which a UPGMA dendrogram showed the same cluster. MEGA X [3] computes pairwise distance (0.01) and overall mean distance (0.01) of substitution type is nucleotides between a query and the database sequences were constructed. The optimal tree with the sum of branch length = 0.036 showed a homogenous pattern among lineages. Conclusion: This study confirms the limited genetic diversity of G. affinis, across 6 sampling sites which might be used in the future to control mosquitofish (G. affinis) and the propagation of its parasitic Lernaea sp.
  Md. Mer Mosharraf Hossain , Md. Imtiaz Uddin , Md. Monjur Hossain , Habiba Islam , Al-Amin , Nawshin Farjana and Rukaiya Afroz
  Background and Objectives: Tilapia lake virus (TiLV) has been marked as an emerging infectious agent that causes mass die-offs in farmed mono-sex Nile tilapia (Tilapia niloticus) in Bangladesh, indicates rapid diagnostic assay. This study was aimed to develop molecular detection method to confirm the TiLV in Tilapia niloticus and construct a genetic baseline to control this disease. Materials and Methods: The research aims to the detection of TiLV followed by complementary techniques of PCR based approaches such as reverse-transcription polymerase chain reaction (RT-PCR) and RT-quantitative (q) PCR using SYBR Green I dye. The RNA quantification, followed by a PCR protocol entailing, complementary deoxyribonucleic acid (cDNA) synthesis and detection of TiLV by either conventional PCR or quantitative identification via qPCR using SYBR Green I dye. Results: This research reported a novel RNA virus allowing its clinical signs lethargy, skin erosion, exophthalmia, detached scales and 15-82% morbidity rate. The RT-PCR amplified a 491 bp fragment from segment 3 in both cases. The PCR amplification efficiency of 98.5% over a wide linear range of 2.98×101 to 2.98×1010 TiLV copies, while the NTC (no template control) produced no fluorescence and therefore no amplification. The sequence of amplified PCR products received 100, 98 and 97% identity. The phylogenetic relationship of 17 TiLV sequences was chosen to compare with GeneBank resulting a common ancestor while closely related with Columbia, India, Malaysia and Thailand. The highest pair-wise alignment score was received 90.20 for MH338228.1 (Columbia), 85.57 for MF582636.1 (India), 85.30 for MH213048.1 (Malaysia) and 86.93 for MH213039.1 (Thailand) using the sequence of TiLV segments of one TiLV-positive strain. Conclusion: The mono-sex Nile tilapia was infected with common fish pathogens, such as Aeromonas and Streptococcus. This newly developed SYBR Green-based RT-qPCR assay can be as an essential tool for TiLV diagnostics and should help to control the dissemination of this virus worldwide.
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