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Articles by Md. Mer Mosharraf Hossain
Total Records ( 4 ) for Md. Mer Mosharraf Hossain
  Md. Mer Mosharraf Hossain , Md. Imtiaz Uddin , Md. Ariful Haque Rupom , Jannatul Ferdoush , Md. Monjur Hossain , Subrata Mondal and Md. Anisur Rahman
  Background and Objectives: To improve the sustainable management and control of North American, western mosquitofish (Gambusia affinis) in the freshwater of Bangladesh, the most invasive to indigenous species, caused the severe infestation to all carps, genetic diversity of the species were studied using DNA sequences of nucleotides first time in Bangladesh. This study was aimed to develop molecular detection method to confirm the G. affinis in freshwater and construct a genetic baseline to control this species. Materials and Methods: This study consisted of 6 microsatellites nuclear DNA loci that were used for the construction of a genetic baseline used a gene-specific marker (cytb). A total of 6 fish for 6 sites were used for DNA extraction, then genotyped and analyzed to examine genetic diversity to assess the persistence of a species in the environment. Results: Genetic diversity was inferred as some polymorphism and monomorphism with minimal genetic distance, in which a UPGMA dendrogram showed the same cluster. MEGA X [3] computes pairwise distance (0.01) and overall mean distance (0.01) of substitution type is nucleotides between a query and the database sequences were constructed. The optimal tree with the sum of branch length = 0.036 showed a homogenous pattern among lineages. Conclusion: This study confirms the limited genetic diversity of G. affinis, across 6 sampling sites which might be used in the future to control mosquitofish (G. affinis) and the propagation of its parasitic Lernaea sp.
  Md. Mer Mosharraf Hossain , Md. Imtiaz Uddin , Md. Monjur Hossain , Habiba Islam , Al-Amin , Nawshin Farjana and Rukaiya Afroz
  Background and Objectives: Tilapia lake virus (TiLV) has been marked as an emerging infectious agent that causes mass die-offs in farmed mono-sex Nile tilapia (Tilapia niloticus) in Bangladesh, indicates rapid diagnostic assay. This study was aimed to develop molecular detection method to confirm the TiLV in Tilapia niloticus and construct a genetic baseline to control this disease. Materials and Methods: The research aims to the detection of TiLV followed by complementary techniques of PCR based approaches such as reverse-transcription polymerase chain reaction (RT-PCR) and RT-quantitative (q) PCR using SYBR Green I dye. The RNA quantification, followed by a PCR protocol entailing, complementary deoxyribonucleic acid (cDNA) synthesis and detection of TiLV by either conventional PCR or quantitative identification via qPCR using SYBR Green I dye. Results: This research reported a novel RNA virus allowing its clinical signs lethargy, skin erosion, exophthalmia, detached scales and 15-82% morbidity rate. The RT-PCR amplified a 491 bp fragment from segment 3 in both cases. The PCR amplification efficiency of 98.5% over a wide linear range of 2.98×101 to 2.98×1010 TiLV copies, while the NTC (no template control) produced no fluorescence and therefore no amplification. The sequence of amplified PCR products received 100, 98 and 97% identity. The phylogenetic relationship of 17 TiLV sequences was chosen to compare with GeneBank resulting a common ancestor while closely related with Columbia, India, Malaysia and Thailand. The highest pair-wise alignment score was received 90.20 for MH338228.1 (Columbia), 85.57 for MF582636.1 (India), 85.30 for MH213048.1 (Malaysia) and 86.93 for MH213039.1 (Thailand) using the sequence of TiLV segments of one TiLV-positive strain. Conclusion: The mono-sex Nile tilapia was infected with common fish pathogens, such as Aeromonas and Streptococcus. This newly developed SYBR Green-based RT-qPCR assay can be as an essential tool for TiLV diagnostics and should help to control the dissemination of this virus worldwide.
  Md. Mer Mosharraf Hossain , Kenji Kawai and Syunichirou Oshima
  To find out adequate inactivator in substitution for the high concentration of formalin, different organic and inorganic chemicals, heat and chemicals combined with heat were tested for the inactivation of a fish-pathogenic bacterium Edwardsiella tarda strain. The survivality, sustainability of the cell surface antigenicity, bacterial protein and the total cell antigens of E. tarda were determined after treated with those inactivators. A major antigen at 37 kDa was detected by Sodium Dodecyl Sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE) and Western blot analysis. Formalin (0.1%) with heat (70°Cx10 min) and 0.9% citric acid killed E. tarda resulted moderate killing activity with enough antigen-sustainability. This study suggests that formalin (0.1%) combined with heat (70°Cx10 min) and citric acid (0.9%) killed E. tarda as a new vaccine candidate.
  Md. Mer Mosharraf Hossain and Kenji Kawai
  This study aimed to design to evaluate the immunogenicity as well as the stability of inactivated bacterins in storage conditions to prevent edwardsiellosis in fish species. Three vaccine formulations, formalin (0.4%, FKC), pressure (600 kgf cm-2 for 5 min, PKC) and electric current (100 mA at 12v DC for 5 sec, ECKC) killed bacterin and a routes of administration with intraperitoneal injection (i.p.) was tested. The effectiveness of the immunization strategies was evaluated in terms of Relative Percent Survival (RPS) and antibody levels. On the basis of the results pressure inactivated vaccine via i.p. which confers RPS values over 85% at least 6 months post-vaccination. In the search for a more stable bacterin, inactivated E. tarda antigen was subjected to different storage temperatures. Storage at 4°C did not significantly affect the titer of PKC and antigenic potency remained stable for 6 month. However, with the bacterins FKC and ECKC there is a considerable loss of potency during stored at 4°C. Bacterins were discarded if exposed to temperature of 0°C or below, because the precise freezing point is not established. Bacterins loosed significant potency after 1 month when stored at 25°C.
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