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Articles by Md. Kamruzzaman Pramanik
Total Records ( 2 ) for Md. Kamruzzaman Pramanik
  A.N.M. Mamun-Or-Rashid , Biplab Kumar Dash , Md. Nurul Abadin Chowdhury , Momtaz Fatima Waheed and Md. Kamruzzaman Pramanik
  The present study was carried out to explore baker’s yeasts strains from sugarcane juice to assess its potential in laboratory scale production of breads. Collected juice samples were processed for isolation and identification of yeast strains based on standard cultural, morphological and biochemical characteristics. Among the six isolated strains, four (designated as S1, S2, S5 and S6) were identified as Saccharomyces cerevisiae and the rests (designated S3 and S4) were as S. rouxii. When assessing their CO2 production rates as a measure of their baking potential, S6 was found to produce maximum amount of gas (226.67 mm3 mL-1) in sucrose broth, whereas gas produced by S2, S1 and S5 were relatively insignificant (170, 136.67 and 86.67 mm3 mL-1, respectively). No strain was found to produce undesirable H2S gas responsible for off-flavor. Besides, effects of different physicochemical parameters (e.g., pH, temperature, substrate concentration, incubation period, agitation etc.) on the production of yeast cell-mass were studied. Yield of cell mass was indirectly measured by spectrophotometric method at 550 nm. All the test isolates were found to produce maximum cell mass at a pH range of 4.0 to 5.0 in 2 to 4% molasses broth at 30°C after 4 days of incubation. In the laboratory scale production of bread using composite flour, Isolate-S6 formed significant characteristic texture. Considering overall characteristics, Isolate- S6 was found to be satisfactorily potent for baking purpose.
  Md. Kamruzzaman Pramanik , Abdullah-Al- Mahin , Mahfuza Khan and Abdul Bathen Miah
  Tephritidae fruit flies usually harbour different bacterial symbionts in their digestive system. These symbionts have been known to play significant role in different fitness parameters of flies. In the present study bacterial symbionts from the mid-gut of laboratory host reared Oriental fruit fly (Bactrocera dorsalis) (Hendel) were isolated and identified using conventional culture based techniques. The mid-gut of ten days old adult female was dissected and processed aseptically and initially total viable bacterial count was determined using nutrient agar medium. Different and discrete colonies on this media were isolated and subcultured. Pure culture of each isolate was subjected to a series of different morphological, cultural and biochemical tests for identification. Total viable bacterial count was revealed as 4.5x105 CFU mL-1 in the mid-gut of B. dorsalis. Eleven bacterial genera with thirteen species were identified. The identified bacterial gerera of B. dorsalis were Listeria, Citrobacter, Moraxella, Proteus, Streptobacillus, Enterobacter, Serratia, Vibrio, Aeromonas, Klebsiella, Morganella. Experimental result reported the presence of Moraxella and Streptobacillus, Aeromonas and Morganella for the first time in mid-gut of laboratory host reared B. dorsalis.
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