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Articles by Md. Ibrahim Khalil
Total Records ( 2 ) for Md. Ibrahim Khalil
  Sudip Paul , Md. Sakib Hossen , E.M. Tanvir , Md. Aminul Islam , Rizwana Afroz , Istiyak Ahmmed , Moumoni Saha , Siew Hua Gan and Md. Ibrahim Khalil
  In this study, the antioxidant potential of Citrus macroptera fruit was compared between its pulp (CMPU) and peel (CMPE). The biochemical effects of an ethanol extract of CMPU were investigated on major organs, including the liver, kidney and pancreas. Male wistar rats (n = 24) were randomly divided into four groups, including Group I (control), Group II (250 mg kg–1), Group III (500 mg kg–1) and Group IV (1000 mg kg–1) and they were administered the extract for 28 days. The CMPE contained higher amounts of total polyphenols (620.91±7.75 mg), flavonoids (508.33±5.49 mg), tannins (585.99±4.46 mg) and protein (4.00±0.14 g) compared to the pulp (291.06±10.14, 145.02±0.36, 526.08±3.32 mg/100 g and 2.89±0.32 g/100 g, respectively). Similarly, CMPE also exhibited higher 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) scavenging activity and Ferric Reducing Antioxidant Power (FRAP) values than CMPU. However, the ascorbic acid content was higher in CMPU (120.83±0.0019 mg/100 g) than CMPE. In vivo studies confirmed that CMPU possessed a significant lipid lowering activity that occurred in a dose dependent manner and that it caused beneficial changes in several other biochemical parameters. Additionally, a significant diminution of lipid peroxidation in liver and kidney tissues was observed. It was concluded that C. macroptera possesses high antioxidant potential and is relatively safe. Its protective effects against various chronic diseases that are associated with oxidative stress should be further investigated.
  Md. Ibrahim KHALIL , Mia Mahmudul HOQUE , Mafroz Ahmed BASUNIA , Nadia ALAM and Md. Asaduzzaman KHAN
  Bioconversion of cellulosic components into fermentable sugars has currently been accomplished with the help of the microbial enzyme, cellulase. In this study, Pleurotus ostreatus (Jacquin ex Fr.) Kummer and Pleurotus sajor-caju (Fr.) Singer were tested for cellulase production by solid state fermentation (SSF) using saw dust, sugarcane bagasse, and paddy straw as substrate. To enhance production, the substrates were pretreated with 1% and 4% NaOH solutions. Supernatants of extracted enzyme solutions were used to estimate reducing sugar and soluble protein, as well as enzyme activities. Maximum production of reducing sugar and soluble protein were recorded in 12 and 10 day fermented products, respectively. In the production of reducing sugars, P. ostreatus (42.8 mg g-1) was found to be more efficient than P. sajor-caju (10.9 mg g-1), while P. sajor-caju was more efficient in soluble protein production (40.2 mg g-1) compared to P. ostreatus (3.1 mg g-1). P. ostreatus was found to be more efficient in the individual components of cellulase enzyme production than P. sajor-caju. All the enzyme activities were found to attain a peak at 10 days of fermentation. The highest activities of endoglucanase, exoglucanase, and β-glucosidase were recorded as 7.08, 7.36, and 3.60 units (μmole of glucose released/min/g substrate), respectively, for P. ostreatus, while that of P. sajor-caju were 1.9, 2.03, and 2.63 units, respectively. Total cellulase activity was found to reach a maximum at 10 days of fermentation for both strains. For P. ostreatus it was recorded as 3.51 units, while for P. sajor-caju it was 0.82 units. This study suggests P. ostreatus as an important source of cellulase enzymes.
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