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Articles by Md. Farid Uddin
Total Records ( 2 ) for Md. Farid Uddin
  Md. Farid Uddin , Shah Md. Asraful Islam , Naznin Sultana and M.H. Kabir Shiragi
  The experiment was conducted in the Tissue Culture Laboratory of the Department of Crop Botany, Bangladesh Agricultural University, Mymensingh for micropropagation of tomato plant to evaluate the effect of variety and Plant Growth Regulators (PGRs) in MS medium on callus proliferation from infected tomato plant. Three tomato varieties namely, Bahar, Binatomato-2 and Binatomato-3 were used as plant materials in the present study. Virus infected young internode segments of tomato plants were cultured on MS medium supplemented with different concentrations and combinations of Plant Growth Regulators (PGRs) for callus proliferation. The combination of 0.5 mg L-1 NAA + 2.0 mg L-1 BAP in MS medium was found the best for inducing healthy and light yellow callus after 15 days culture and callus turned into yellow at 30 Days After Inoculation (DAI) in all varieties. Calli were fully virus infected which was confirmed by ELISA test. These calli can be used for the production of virus free tomato plant by shoot derived meristem culture.
  Md. Farid Uddin , Md. Abu Taher , Shah Md. Asraful Islam and Md. Zakir Hossain
  The experiment was conducted in the Tissue Culture Laboratory of the Department of Crop Botany, Bangladesh Agricultural University, Mymensingh, Bangladesh for micropropagation of tomato to evaluate the effect of variety and plant growth regulators in MS medium on shoot induction from virus infected calli of tomato plants. Three tomato varieties namely Bahar, Binatomato-2 and Binatomato-3 were used as plant materials in the present study. Callus derived shoots were induced on MS medium supplemented with different concentrations and combinations of plant growth regulators (PGRs). The combination of 0.2 mg L-1 IAA+4.0 mg L-1 BAP in MS medium was the best for inducing shoots which turned green to dark green after 15 days of culture. Callus derived shoots were fully virus infected which was confirmed by ELISA test. Meristem of plantlet can be used for the production of virus free tomato plant by meristem culture.
 
 
 
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