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Articles by May M. Amer
Total Records ( 2 ) for May M. Amer
  Hamdy A. Shaaban , Hatem S. Ali , Gamil F. Bareh , Abdel Rahman S. Al-khalifa and May M. Amer
  Background: Nowadays, packaging research is receiving considerable attention because of the development of eco-friendly materials made from natural polymers such as chitosan (CH) and hydroxypropyl methylcellulose (HPMC). Essential oils are effective antimicrobials on important some pathogenic bacteria and can be added packaging materials due to absorb various surfaces. Objective: The main purposed of this study was to prepared antimicrobial films by incorporating different concentrations of marjoram, clove and cinnamon essential oils, into chitosan and hydroxypropyl methylcellulose films against foodborne pathogens. Methodology: Chitosan (1% w/w) was dispersed in an aqueous solution of glacial acetic acid (0.5% w/w) at 25°C. Following overnight agitation, essential oils were added to the chitosan solution. Hydroxypropyl methylcellulose 1% weight was dispersed in de-ionized water at 80°C. After the dissolution of the polysaccharide, essential oils were added. Essential oils clove (Syzgium aromaticum), marjoram (Origanum majorana ) and cinnamon (Cinnamomum zeylanicum ) were extracted by hydro-distillation. The antibacterial effects of essential oils were studied against three important food pathogens, Escherichia coli, Staphylococcus aureus and Listeria monocytogenes by application of agar diffusion method. Also, antimicrobial effectiveness of films were studied by tryptone soy agar with 3% NaCl was used as a model solid food system (TSA-NaCl). Results: The intensity of antimicrobial efficacy was in the following order: Marjoram>clove>cinnamon. The antibacterial effectiveness of the prepared films against E. coli , S. aureus and L. monocytogenes was studied at 10°C during 12 days. The HPMC-EO and CH-EO composite films present a significant antimicrobial activity against the three pathogens considered. In all film matrices, marjoram exhibited the highest antimicrobial activity. A complete inhibition of microbial growth was observed for CH or HPMC-marjoram films for E. coli , HPMC-marjoram for L. monocytogenes and HPMC-clove for S. aureus . Conclusion: The HPMC-EO and CH-EO composite films, containing clove, cinnamon or marjoram, showed a significant antimicrobial activity (bacteriostatic effect) against the three pathogens studied (E. coli , L. monocytogenes and S. aureus ). In all film matrices, marjoram exhibited the highest antimicrobial activity.
  Sherif S. Mohamed , Lamiaa E. El-Sedeek , Mohamed M. Sief , May M. Amer , Sherif R. Mohamed and M.H.M. Abdel-Aziz
  Background and Objective: Formalin can be credited for raising the number of patients with kidneys failure, which is increasing in incidence in Egypt annually, according to WHO and the Egyptian Ministry of Health. Lemon grass Cymbopogon schoenanthus (CS) is highly reputed in Egyptian folk medicine as an effective renal antispasmodic and diuretic agent. This study aimed to use the CS extract to eliminate the formalin hazard in rats. Materials and Methods: The experiment was designed and rats divided into six groups are group 1 (untreated) served as control group; group 2 received formalin solution, group 3 and 4 orally treated with CS extract at different two doses (50 and 100 μL). Groups 5 and 6 received formalin solution plus CS extract at mentioned doses. The changes in rat's body weight were noted and the biochemical blood parameters were determined as well as histopathological examination of liver and kidney. Results: The results indicated that all tested biochemical parameters were in normal value for the control group and had no significant differences for CS groups. While, the animals treated with formalin at dose of (100 mg kg–1 b.wt.) showed significant increase in tested biochemical parameters compared with control and CS groups. Conclusion: It is worthy to mention that the rats treated with CS plus formalin showed significant changes in all tested parameters compared with the group received formalin alone. The histopathological examination confirmed the blood biochemical analyses and treatment with CS extract improved the liver and kidney tissues.
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