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Articles by Matsuzaki Tomohiro
Total Records ( 2 ) for Matsuzaki Tomohiro
  Bimal Kumar Pramanik , Toshiyuki Matsui , Haruo Suzuki , Yusuke Kusugi and Matsuzaki Tomohiro
  This study investigated the seasonal fluctuations in invertase, sucrose synthase (SS) and sucrose phosphate synthase (SPS) activities and sugar and organic acid contents in the florets and branchlets of two broccoli cultivars ‘Hartland’ and ‘Sairin’ harvested from October to April. In both, cultivars and portions, invertase (soluble and cell wall-bound fraction) and SS activities were high in October thereafter decreasing until January and once again gradually increasing up to April. In both cultivars, there was no specific change in SPS activity throughout the harvest season but the activity was high in January. Organic acids (malic and citric) and sugar (specially, sucrose) concentrations increased from October to a maximum in January and again decreased up to April. However, no significant change was observed in glucose and fructose. There was a highly significant negative correlation observed between invertase and SS activities and sucrose content in the florets and branchlets of both cultivars. ‘Sairin’ showed higher invertase, SS and SPS activities in both florets and branchlets than ‘Hartland’.
  Dewoowoogen Porras Baclayon , Toshiyuki Matsui , Haruo Suzuki , Yusuke Kosugi and Matsuzaki Tomohiro
  Temperature changes during growth and development influence various metabolic processes in plants. To determine its influence in broccoli (Brassica oleracea L.) at harvest, we examined the activities of some ammonia-assimilating enzymes and ammonia content in the floret and branchlet portions of two cultivars (Hartland and Sairin) harvested from October to April. Glutamine synthetase (GS; EC 6.3.1.2) in the two portions showed decreasing and increasing activity in the florets and branchlets, respectively with decrease in temperature. Asparagine synthetase (AS; EC 6.3.5.4) activity did not show significant variation in both cultivars throughout the experimental period except the branchlet portion wherein activity reached a peak in January and then fluctuated until April. AS activity was significantly higher in the floret than in the branchlet portion in both cultivars. Glutamate dehydrogenase (GDH; EC 1.4.1.2) aminating activity was higher during warmer harvest months while no distinct declining or inclining trend was found in the deaminating activity throughout the experimental period. GDH activities were significantly higher in the branchlet than in the floret portion except for GDH-amination in Hartland harvested in October and December. In the floret portion GDH activity was not detected in Sairin and Hartland after December and February harvests, respectively. The results of this study may provide insights in obtaining optimum yield and product quality of broccoli.
 
 
 
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