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Articles by Masuder Rahman
Total Records ( 1 ) for Masuder Rahman
  Masuder Rahman , Bytul M. Rahman and Bahanur Rahman
  A total of 17 Escherichia coli isolated from 24 fresh faecal samples of broiler and layer were screened for their antibiograms and plasmid profiles. The overall recovery rate of E. coli from faecal samples was 70.83%. All E. coli strains were analyzed to determine their susceptibility patterns to 8 commonly used antibiotics (ampicillin, cephradine, chloramphenicol, ciprofloxacin, gentamicin, streptomycin, tetracycline and sulphamethoxazole) belonging to different groups. From the antibiogram study it was revealed that 87.50% E. coli isolated from broiler were resistant to both ampicillin and sulphamethoxazole. Only 37.50% broiler isolates were highly sensitive to gentamicin and 50% isolates to chloramphenicol. All the E. coli isolates of layer were completely resistant (100%) to sulphamethoxazole and about fifty 5% of the isolates (55.55%) were resistant to both streptomycin and tetracycline. E. coli isolated from layer were found to be highly sensitive (44.44%) to chloramphenicol and 66.66% were also highly sensitive to gentamicin. Plasmid profile of 17 isolates was analyzed by 0.8% agarose gel electrophoresis. A total of 8 different plasmid bands of different size were estimated by eye comparing to reference marker. The estimated size of the bands were 3.25, 5.20, 6.00, 8.00, 15.0, 30.0, 33.5 and 38.0 kbp. Plasmid profile analysis of the isolated E. coli revealed that the isolates carrying multiple plasmids which might be the cause of various degrees of antibiotic resistant. The plasmids were distributed at random in the isolated E. coli strains and there was no remarkable interrelationship between antibiotic resistance and plasmid present. In most of the cases, strains having similar plasmid bands but confer resistant to different antibiotics. In some cases, isolates showed resistance to antibiotics but did not harbor any plasmid indicating that chromosomal DNA may carry the genes that confer resistance to antibiotics.
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