Asian Science Citation Index is committed to provide an authoritative, trusted and significant information by the coverage of the most important and influential journals to meet the needs of the global scientific community.  
ASCI Database
308-Lasani Town,
Sargodha Road,
Faisalabad, Pakistan
Fax: +92-41-8815544
Contact Via Web
Suggest a Journal
Articles by Masood Akhtar
Total Records ( 2 ) for Masood Akhtar
  Masood Akhtar , M. Mazhar Ayaz , C.S. Hayat , Abdul Jabbar , M. A. Hafeez , M. Ashfaque and I. Hussain
  The involvement of secretary immunoglobulin system in the protection of mucous surfaces by locally produced antibodies lead to the demonstration of IgA. IgA in the intestine of immune birds may limit coccidian replication. Enzyme linked immunospot (ELISPOT) assay was employed to detect the mucosal immune response (IgA antibody secreting splenic cells) in chicken vaccinated with egg adapted gametocytes antigen. Twenty to twenty-five % (200,000 to 250,000/106 cells) of chicken splenic cells stimulated with gametocytes antigen were detected as IgA secretors. Results of the ELISPOT revealed that spleen is one of the major source of cells producing IgA in chickens. Such a high number of IgA antibody secreting cells indicate protection in birds vaccinated with gametocytes antigen via oral route against coccidiosis. ELISPOT assay is found to be an advantageous alternative to conventional haemolytic plaque technique, being simpler and of equivalent or even greater sensitivity.
  Masood Akhtar , C.S. Hayat , M. Ashfaque , Iftikhar Hussain , Murad Ali Khan and Sultan Ayaz
  A splenic cell migration inhibition test with modifications was successfully employed in the flat bottom micro titration plates to detect the cell mediated immunity more conveniently and accurately due to vaccine against coccidiosis. A drop of melted agarose (0.2% in PBS) was used to immobilize the splenic fragments. The splenic cells were culture in Hank`s Balanced salt containing fresh chicken plasma and maintained for 10 days. Twenty four hours after cultivation, dominant cell type migrated from a splenic fragment was leukocyte-like cells. At the fourth day these appeared predominantly large round cells and resembled to the macrophage. At the 10th day, spindle cells resembling of fibroblast appeared. The migration of the splenic cells from immunized chickens were inhibited remarkably with the antigen and there was a significant difference (P<0.01) in migration distance of the splenic cells with and without the antigen.
Copyright   |   Desclaimer   |    Privacy Policy   |   Browsers   |   Accessibility