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Articles by Mariana Nor Shamsudin
Total Records ( 8 ) for Mariana Nor Shamsudin
  Sobhan Ghafourian , Nourkhoda Sadeghifard , Zamberi bin Sekawi , Vasantha Kumari Neela , Mariana Nor Shamsudin , Reza Mohebi , Mohammad Rahbar and Mohammad Raftari
  Problem statement: Gram-negative pathogens harboring ESBLs have caused numerous outbreaks of infections and are becoming an increasing therapeutic problem in many countries. The incidence of ESBL-producing strains among clinical isolates has been steadily increasing over the past years resulting in limitations of therapeutic option. The focus of this study was to examine the molecular epidemiology of ESBL-producing Klebsiella spp, investigate the susceptibility of Klebsiellae spp producing ESBLs towards non-beta-lactam antibiotics in the different seasons, identify the various clonal types of ESBL-producing K.pneumoniae and detect the dominant ESBL clonal types. Approach: Clinical isolates of Klebsiella spp were identified during the period March 2007-April 2008. ESBLs production identified by phenotypic and genotypic methods. MLST was performed for dissemination of ESBLs producing K. pneumoniae. Results: The findings showed that 51.6% of K.pneumoniae were produces ESBLs. 35.8, 21.2 and 38.7% of K. pneumoniae producing ESBLs were resistant to amikacin, ciprofloxacin and cotrimoxazol, respectively. It was found that 40 and 27.3% of K.oxytoca producing ESBLs were resistant to cotrimoxazol and amikacin, respectively. The findings reflected that ESBLs existed in 73% of K. oxytoca. The results showed that the frequency of blaSHV, blaTEM and blaCTX-M due to K.pneumoniae producing ESBLs were 87.5, 12.4 and 24.8%, respectively. Of the eleven K. oxytoca producing ESBLs, 100% blaSHV were obtained. Based on the nucleotide variations of the five genetic loci, twenty-five different STs could be identified among thirty K.pneumoniae producing ESBLs isolates. Among the STs shared by multiple isolates, the most frequently encountered were 14, 16 and ST18. Conclusion: In conclusion, the percentage of K.oxytoca producing ESBLs was higher than K.pneumoniae producing ESBLs. Generally, K.penomoniae produces more ESBLs in winter and fall than in the other seasons.
  Siti Fatimah Binti Sulaiman , Khairul Afizi , Mohd Salim , Mariana Nor Shamsudin and Yasser Mohamed Abdelhadi
  The antibacterial activity of certain commercial antibiotics and common herbs was evaluated against pathogenic Pseudomonas fluorescens, Pseudomonas putida and Pseudomonas aeruginosa isolated from Malaysian and Egyptian cultured fish, mainly tilapia. A suspension of freshly cultured isolates was prepared (with 0.5 OD) and 100 μL of this suspension was spread over the Muller’s Hinton agar plates. The antibiotic discs were inoculated on each cultured plate while the herbal extracts were soaked on Whatman filter paper (20 μL each) that have been cut into discs and later inserted on to bacteria-cultured plates to screen their sensitivity to both antibiotics and herbs. Double-fold dilution was used to determine the Minimal Inhibitory Concentration (MIC) for the effective herbs at 100, 50, 25, 12.5 and 6.25%. Results revealed high resistance of the tested bacteria against most of the screened antibiotics except Ciprofloxacin. With regard to herbal sensitivity, only Origanum vulgare showed effectiveness and inhibition zone against all isolates. The MIC ranged from 15-40% for both Egyptian and Malaysian isolates. Thus, Origanum vulgare is recommended as a feed additive for cultured fish and can also be applied for inactivated and live-attenuated Pseudomonas vaccines’ preparation.
  M. Aminur Rahman , Fatimah Md. Yusoff , A. Arshad , S.M.N. Amin and Mariana Nor Shamsudin
  An attempt was undertaken to describe the size frequency distribution, length weight relationships and fecundity estimates of short-spined sea urchin, Salmacis sphaeroides from Peninsular Malaysia. In total 355 specimens of S. sphaeroides were collected from the inter-tidal shoal of Tanjung Kupang, Johor during the breeding season in March-August 2011. For each individual, the Total Length (TL) was measured using digital slide calipers and individual Body Weight (BW) was also taken through a digital balance. Mean length of S. sphaeroides was estimated as 72.85 mm and the mean weight was 143.01 g. Among the 355 specimens measured, 158 were males and 197 were females, indicating a sex ratio of 1 male to 1 female (1:1.25). The logarithmic form of length-weight relationship of S. sphaeroides was LogW = 2.4396xLogTL-2.3958. The exponential form of equation obtained for the length-weight relation was W = 0.004xTL2.4396. The value of regression co-efficient (R2) estimated for the species was 0.77. The mean fecundity was estimated at 7,676,000 (±247773) eggs. The regression coefficient between the absolute Fecundity (F) and Total Length (TL) was 0.76 and between the fecundity and drained body weight (DW) was 0.89; revealed linear regression model with a positive and significant relationship. This study represents the first time results on the total length-body weight relationships and fecundity estimates of this sea urchin from Peninsular Malaysia. The findings would immensely be helpful towards the understanding of growth patterns and fecundity, which will ultimately facilitate to develop the breeding, larval rearing and aquaculture of sea urchins.
  Nor Asmara Tasrip , Mohd Nasir Mohd Desa , Cheah Yoke Kqueen , Nurul Zarith Mohamad Zin , Zainul Amiruddin Zakaria , Zarizal Suhaili , Chong Pei Pei and Mariana Nor Shamsudin
  This study compared the distribution of aerial mass colors, antibacterial activities and RAPD-based genomic patterns of 39 Streptomyces isolates harvested mainly from soil at the University Agricultural Park, Universiti Putra Malaysia. Streptomyces griseus ATCC 10137 strain was also included as a reference strain (n = 40). Based on ISP-2 media, the aerial mass colors observed were categorized as yellow (n = 15), grey (n = 9), brown (n = 7), white (n = 6) and others (n = 3). Antibacterial activities were assessed on Mueller Hinton Agar (MHA) and Tryptic Soy Agar (TSA) by perpendicular streak method against Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, Salmonella sp. and Enterococcus sp. MHA demonstrated 15 isolates with broad spectrum antibacterial activities and 18 as non-broad spectrum. TSA gave lower proportions with 15 and 9 isolates respectively. Regardless of the test media used, a higher proportion of isolates with non-white color showed antibacterial activities to suggest a potential correlation. RAPD dendrogram (a composite of 3 random primers) also clustered majority of them but segregated those of white color, which showed less antibacterial activities, in a different cluster. Further validations involving more isolates are warranted to establish the findings.
  Y.M. Abdel-Hadi , Mariana Nor Shamsudin , K. Yusoff and Shater Zakaria
  Monovalent, killed and live attenuated vaccines of Aeromonas hydrophila and Pseudomonas putida were used in the immunization of red tilapia against Motile Aeromonad and Pseudomonad septicemias. There were 4 treatments and a 5th control group with 3 replicates per each. A 4th replicate was kept for replacement of natural mortality among the experimented fish. The 4 treatments included, Heat-killed vaccine of A. hydrophila, Live-attenuated vaccine of A. hydrophila (using herbs), Heat-killed vaccine of P. putida and Live-attenuated vaccine of P. putida. A total of 160 brood stocks of O. niloticus with 250 g average body weight were used for all treatments (8 fish per each glass aquarium). Vaccination was conducted via the Intra Peritoneal route (I/P) as an initial dose followed by 2 booster doses every 2 weeks. The last dose was applied via the immersion route. The evaluation of vaccination was carried out through periodical antibody titration of the serum of the examined fish (every 2 weeks) using direct agglutination method as well as by the experimental challenge 3 months after the initial immunization. Results revealed that there were a significant difference between the vaccinated and non vaccinated fish of the control group regarding antibody titers and Relative Percent Survival (RPS) of the challenge test. Differences in immunity levels within the vaccinated groups themselves were demonstrated.
  Nik Khairul Azizi Nik Ibrahim , Mariana Nor Shamsudin and Zamberi Sekawi
  The current research trend for a new drug development was expanded by using the proteomic approaches. However, the expression on those drug target must be well identified before taking part into proteomics study. One of the most commonly use protocol is the expression profiles on those selected genes during certain simulated conditions. Staphylococcus aureus has been blessed with several virulence factors which are essential for its pathogenicity. One of the most well studied systems is the Two-Component Regulatory Systems (TCRSs). The TCRSs play a vital role in S. aureus survival and it become a good candidate for the specific drug target. Prior to determining the role, this study was made to observe the expression pattern of two TCRS’ regulatory genes during the treatment with antibiotics against two strains of S. aureus; the Methicillin Methicillin Susceptible S. aureus (MSSA) and Methicillin Resistant S. aureus (MRSA). The partial transcripted analysis of the Reverse Transcriptase PCR (RT-PCR) data shows that the rates of expression in selected TCRS genes were different in both strains. Knowing the importance to S. aureus growth and pathogenesis, the TCRS genes can be a good candidate for designing a novel specific drug target against S. aureus, especially the MRSA.
  Nagi A. AL-Haj , Mariana Nor Shamsudin , Raha Abdul Rahim , H. Halimaton , Lai L. Suang , M. Nurmas I. Mashan and Zamberi Sekawi
  Vibrio cholerae has caused severe outbreaks of cholera worldwide with thousands of recorded deaths annually while the Staphylococcus aureus is one of the most significant pathogens causing nosocomial and community-acquired infections. Conventional detection methods for diagnosis of clinical samples, water and food based on culture, microscopy and biochemical testing are limited by the speed of detection, sensitivity and specificity, so it is necessary to develop innovative molecular methods for the rapid detect the presence genes, expression levels of the toxigenic and drug target genes in S. aureus and V. cholerae using PCR, sequencing and membrane array. The genes studied are SEA-SEJ (genes encoding S. aureus enterotoxins) ace, zot, ctxA, ctxB, toxR (toxigenic genes of V. cholerae) Sav1017 and AdaB (protein synthesis and DNA synthesis genes in S. aureus. These techniques were carried out step by step with primers designing, PCR amplification, sequencing and detection of expression by membrane array. These assays are extremely robust, sensitive, specific and economical and can be adapted to different throughputs. Thus, a rapid, sensitive and reliable technique for detecting toxigenic genes of S. aureus and V. cholerae was suc-cessfully developed.
  Zamberi Sekawi , Rusmah Yusof and Mariana Nor Shamsudin
  A study was conducted to portray a preliminary characteristic of extended-spectrum β-lactamases (ESBLs)-producing Escherichia coli in a local tertiary hospital in Malaysia. Sixteen clinical isolates of ESBLs producing E. coli from different sources were examined for blaSHV/TEM/CTX-M ESBL genes by PCR molecular assay. Each strain was found to carry at least one of the genes. This study demonstrated a high prevalence of blaCTX-M (81.3%) and blaTEM genes (75%). Only two strains (12.5%) carried the blaSHV gene. Nucleotide and deduced protein sequences determination showed; 61% produced CTX-M-15, 31% produced CTX-M-14 and 8% produced CTX-M-3. The antimicrobial susceptibility testing data determined that almost all sixteen isolates were resistant to oxyimino-cephalosporins, 46% resistant to gentamicin, 69% resistant to trimethoprim-sulfamethoxazole and 46% resistant to ciprofloxacin. This study preliminary emphasizes the epidemiology of the ESBL-producing E. coli particularly SHV, TEM and CTX-M-type producing E. coli in Malaysia.
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