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Articles by Maria Endo Mahata
Total Records ( 17 ) for Maria Endo Mahata
  Yose Rizal , Maria Endo Mahata , Mira Andriani and Guoyao Wu
  An experiment was conducted with 80 unsexed broilers of the Arbor Acress strain to determine the capability of a carrot and fruit juice wastes mixture (carrot, apple, mango, avocado, orange, melon and tree tomato) in the same proportion for replacing corn in broiler diet. This study involved a Completely Randomized Design (CRD) with 5 treatments (0, 5, 10, 15 and 20% of juice wastes mixture in diets) and 4 replicates per treatment. Diets were isonitrogenous (22% crude protein) and isocaloric (3000 kcal/kg diet). Measured variables were feed consumption, average daily gain, feed conversion, as well as percentages of abdominal fat pad, carcass, digestive organs (liver, pancreas and gizzard) and heart. Data were analyzed by analysis of variance for CRD. Increasing juice wastes mixture levels in diets increased feed consumption (p<0.05) and average daily gain (p<0.01), while improving feed utilization efficiency (p<0.05). These treatments also affected (p<0.05) abdominal fat pad percentage but had no effect (p>0.05) on carcass, liver, pancreas, gizzard or heart percentages. In conclusion, up to 20% of juice wastes mixture could be included for the broiler diet to effectively replace up to 40% corn in the diet.
  Maria Endo Mahata , Ichni Lestari , Abdi Dharma , Irsan Ryanto H. , Armenia and Yose Rizal
  Serratia marcescens is a bacterium able to produce chitinase for degrading chitin. Broiler chickens can not digest significant amounts of chitin because they produce very little chitinase in their digestive tract. Therefore, broiler feed containing chitin must be processed first with chitinase. Serratia marcescens is an opportunistic pathogenic bacterium and a pathogenicity test is required before using its chitinase for processing of feed containing chitin. An experiment was conducted by using broiler. A split-plot, completely randomized design is used in this experiment. The Serratia marcescens dosages (0, 40, 80, 160 and 320 mg/kg body weight) were the main plot and observation lengths (2, 4, 8, 16 and 22 days) were the sub plot factor. The ration for different treatments had the same protein (23%) and energy (3000 kkal/kg) content. Feed consumption, average daily gain, body temperature and mortality were parameters. Results showed that feed consumption and average daily gain were significantly affected (p<0.05) by presence of Serratia marcescens. The highest feed consumption (123.10 g/broiler) and average daily gain (76.75 g/broiler/day) were found for the highest bacterium dosage of 320 mg/kg body over 22 days observation. Body temperature range from 39.82-40.08°C and there was no affect of Serratia marcescens on mortality. In conclusion, the Serratia marcescens had no negative effect on broiler performance.
  Maria Endo Mahata , Mailinda Juwita Sasti , Regina Septia Aryani , Yose Rizal and Guoyao Wu
  The Juice Wastes Mixture (JWM) is a combination of waste from carrot and some fruits (apple, mango, avocado, orange, melon and tree tomato) in the same proportion after their juices were squeezed for fresh drink. The JWM could be as an alternative potential feed for replacing corn in the broiler diet. The problem of JWM as broiler feed is the high in crude fiber and low in metabolic energy contents. After processing the JWM by soaking in 20% concentration of rice hull ash filtrate for 72 hr (IJWM), its crude fiber decreased, while its metabolizable energy increased. An experiment was conducted to evaluate the utilization of IJWM in broiler diets. The experiment was performed in a completely randomized design with different levels of IJWM (0, 20, 25, 30, 35 and 40%) for replacing corn in the broiler diets. The variables measured were feed consumption, average daily gain, feed conversion, live body weight, abdominal fat pad percentage, carcass percentage, liver percentage and pancreas percentage. Every treatment was repeated 4 times. The results showed that the IJWM influenced the feed consumption, average daily gain, live body weight, feed conversion and abdominal fat pad very significantly (P<0.01). Meanwhile, liver percentage and pancreas percentage were not affected by the IJWM. In conclusion, the IJWM could be included up to 40% to effectively replace 80% corn in the broiler diets.
  Nuraini , Ade Djulardi and Maria Endo Mahata
  Durian fruit waste (peel and seed) can be used as an alternative feed based on the potential availability and nutrient content. Three experiments were conducted to improve the nutrient quality of durian fruit waste (DW) and tofu waste (TW) mixture through fermentation by using Phanerochaete chrysosporium and Neurospora crassa. The substrates consists of 70% DW (peel and seed in the same proportion) and 30% TW. Experiment 1 was performed in a 3 x 3 factorial experiment of completely randomized design with 3 replicates. First factor was inoculum composition of P. chrysosporium and N. crassa (1: 1, 2: 1 and 1: 2) and second factor was inoculums doses (6, 8 and 10%). Measured variables were Crude Fiber (CF) and Crude Protein (CP). Experiment 2 was to study incubation period (7, 9, 11 and 13 days). Measured variables were DTW’s CF, CP, cellulose, hemi-cellulose and lignin. Experiment 3 was to compare nutrient content between fermented (the best treatment at experiment 2) vs. unfermented one. Measured variables were DTW’s nitrogen retention, amino acid profile, B carotene, tannin and Metabolizable Energy (ME). Result of the experiment 1, there was a very significant (p<0.01) interaction between inoculum compositions and inoculum doses in which P. chrysosporium and N. crassa inoculum composition (1:1) and 8% inoculum dose reduced CF and increased CP content. Experiment 2, incubation period of 9 days reduced CF, NDF, ADF, lignin and cellulose from 22.33, 32.49, 27.50, 15.81 and 17.62 to 8.30, 30.58, 20.26, 6.20 and 11.58%, respectively. Meanwhile increased CP and hemicellulose from 11.73 and 4.99 to 19.37 and 9.52%, respectively. Experiment 3, Nitrogen retention, B-carotene, tannin and ME content of fermented was better than unfermented DTW (42.50 vs 68.54%, 0 vs. 119.07 mg/kg, 3,76 vs 0.43% and 2586.84. vs. 2728.27 kkal/kg, respectively). Phanerochaete chrysosporium and Neurospora crassa treatment improved DTW’s amino acids profile. In conclusion, fermentation by using Phanerochaete chrysosporium and Neurospora crassa (1:1), 8% inoculum dose and 9 day incubation period was the best treatment for improving DTW’s nutrient quality.
  Yose Rizal , Nuraini , Mirnawati , Maria Endo Mahata , Rio Darman and Dendi Kurniawan
  An experiment was conducted to determine the effects of several levels of Neurospora crassa-fermented palm kernel cake in the diet on the production performance of Gold Arab laying-hens and to obtain the appropriate level of this fermented palm kernel cake for reducing the utilization of concentrated feed in the diet. Three hundred Gold Arab laying-hens of 72 weeks old were employed in this experiment and randomly assigned to four treatments (0, 7.25, 10.15 and 13.05% fermented palm kernel cake in diets) in a completely randomized design with five replicates. The measured variable was production performance (feed consumption, egg-mass production, feed conversion, egg weight and hen-day egg production). Results of experiment indicated that feed consumption, egg-mass production, feed conversion, egg weight and hen-day egg production were not influenced (p>0.05) by diets. In conclusion, Neurospora crassa-fermented palm kernel cake could be included up to 13.05% to effectively replace 45% concentrated feed in the diet of Gold Arab laying-hens without adverse effect on the production performance.
  Maria Endo Mahata , Jamaluddin Manik , Muhammad Taufik , Yose Rizal and Ardi
  Owing to the beneficial effects of a carotenoid called lycopene in tomatoes, the present work was undertaken to study the effect of dietary unboiled (UT) and boiled tomato (BT) waste in broiler chicken on performance, internal organ development and serum lipid profile. One hundred fifty Arbor Acress strain broiler chicken of one week age were used for the study. The experiment was performed in a completely randomized design with different combination of UT and BT (0% UT: 0% BT; 25% UT: 75% BT; 50% UT: 50% BT; 75% UT: 25% BT; 100% UB and 100% BT) to completely replace rice-bran and altering the levels of yellow corn and coconut oil in diet. Each treatment was repeated five times. The results revealed that feed consumption, daily weight gain and feed conversion of broiler chicken were not significantly (p>0.05) affected by various dietary combinations of UT and BT waste. The weights of said internal organs of broiler chicken did not vary significantly (p>0.05) by various combination of dietary UT and BT waste. Further, except for HDL, there was a significant (p<0.05) effect on serum total cholesterol, LDL and triglyseride values of broiler chicken fed different combinations of UT and BT waste in diet. In conclusion, boiled tomato waste up to 7% level in the diet of broiler chicken is highly effective in regulation of lipid metabolism in a positive manner and could prove as an important tool for health conscious people in the prevention of atherosclerosis or coronary heart diseases.
  Zurmiati , Wizna , M. Hafil Abbas , Maria Endo Mahata and Revan Fauzano
  Background and Objective: Live probiotic feed additives can balance microorganism populations in the digestive tract. This study aimed to investigate the effects of the probiotic Bacillus amyloliquefaciens on growth performance and feed efficiency of Pitalah starter ducks. Methodology: A completely randomized design was used in this study. Three different doses of B. amyloliquefaciens (1000, 2000 and 3000 ppm) were dispensed in the drinking water of 1 day old Pitalah ducks (N = 25 duck per dose) and each treatment was replicated five times, control birds were given 0 ppm (N = 25 duck). Feed consumption and conversion, body weight gain, income over feed cost, total colony count of Bacillus sp., in the small intestines and acidity in small intestine were measured. Results: The results showed that all three doses significantly decreased feed consumption, feed conversion and small intestinal acidity (p<0.05) and increased income over feed cost and total small intestinal Bacillus sp., colony counts. There was non significant change in weight gain (p>0.05). Conclusion: It is concluded that addition of 2000-3000 ppm of B. amyloliquefaciens to the drinking water of Pitalah ducks can improve the feed efficiency by >15% and provide economic benefits for farmers raising starter ducks.
  Yan Heryandi , Adrizal , Nela Ningsih and Maria Endo Mahata
  Background and Objective: The utilization of pineapple peel waste as poultry feed is limited due to the high crude fiber content. The poultry digestive tract does not produce cellulase, a cellulose digesting enzyme, however, cellulolytic microorganisms produce cellulase that could be used in hydrolyzing high-cellulose feed content through a fermentation method. The previous experiment showed that crude fiber content in pineapple peel waste was reduced from 24-17.16% after fermenting for 1 week with a local microorganism solution derived from bamboo sprouts. Thus, this study aims to evaluate the amount of fermented pineapple peel waste, using a local microorganism solution derived from bamboo sprouts, that could be used in a broiler diet, as well as how it effects the carcass performance and organ development of broilers. Materials and Methods: This experiment was performed with a completely randomized design using five different amounts of fermented pineapple peel waste (0, 3, 6, 9 and 12%) in the broiler diet and each treatment was repeated four times. Body weight, abdominal fat pad percentage, carcass weight and percentage and organ development in relation to the liver, gizzard, spleen and duodenum weight were measured. Results: The inclusion of different amounts of fermented pineapple peel waste in the broiler diet did not significantly affect (p>0.05) the body weight, abdominal fat pad percentage, carcass weight and percentage, organ development, such as weight of the liver, gizzard, spleen and duodenum. Conclusion: Fermented pineapple peel waste using a cellulolytic local microorganism solution derived from bamboo sprouts could be used up to 12% in a broiler diet without negative effects on carcass performance and organ development.
  Maria Endo Mahata , Yose Rizal and Guoyao Wu
  The problem of juice waste mixture as poultry diet was high in crude fiber and low in metabolizable energy contents. Experiments were performed to improve the nutrient quality of juice waste mixture by using steam pressure. The first experiment was designed in a completely randomized design with 4 different incubation times of juice waste mixture (0, 15, 30, and 45 minutes) in Autoclave (15 psi pressure and 121°C), and each treatment was replicated 5 times. Measured variables were crude fiber, crude protein, crude fat. The second experiment was to compare of crude fiber, crude protein, ether extract, NDF, ADF, cellulose, hemi-cellulose, lignin, nitrogen retention, amino acid profile, and metabolizable energy of juice waste mixture between treated vs. untreated one. The results showed that the crude fiber, crude protein, and crude fat of juice waste mixture were significantly affected (P<0.05) by incubation time in autoclave. The incubation time for 30 minutes decreased crude fiber and crude fat, but increased crude protein contents of the juice waste mixture. The crude fiber, ether extract, NDF, ADF, cellulose, and lignin contents between untreated vs treated reduced from 17.10, 6.24, 34.30, 24.40, 12.20, and 11.80% to 12.02, 4.06, 32.62, 22.05,10.50,11.55%, respectively. Meanwhile, nitrogen retention, crude protein, hemi-cellulose, and metabolizable energy increased from 59.99, 8.40, 9.90%, and 1744 kcal/kg to 65.51, 13.35, 10.57%, and 2550 kcal/kg, respectively. In conclusion, the incubation time for 30 minutes in autoclave was the best treatment for improving the nutrient quality of juice waste mixture.
  Yose Rizal , Maria Endo Mahata , Indra Joli and Guoyao Wu
  Two experiments were conducted to improve Juice Wastes Mixture’s (JWM’s) nutrient quality by using Trichoderma viride and to compare between treated vs. untreated one. This JWM consists of carrot, apple, mango, avocado, orange, melon and tree-tomato in the same proportion. Experiment 1 was performed in a 3 x 5 factorial experiment of completely randomized design with 3 replicates. First factor was Trichoderma viride concentration (5, 7 and 9%) and second factor was incubation period (0, 5, 7, 9 and 11 days). Measured variables were Crude Fiber (CF), Crude Protein (CP) and Ether Extract (EE). Experiment 2 was to compare nutrient content between treated vs. untreated one. Measured variables were JWM’s CF, CP, EE, NDF, ADF, cellulose, hemi-cellulose, lignin, nitrogen retention, amino acid profile and Metabolizable Energy (ME). There was a very significant (p<0.01) interaction between Trichoderma viride concentration and incubation period in which 7% Trichoderma viride and 5 day incubation period reduced CF and EE and increased CP content. CF, EE, NDF, ADF, cellulose, hemicelluloses and lignin contents between untreated vs. treated one declined from 17.10, 6.24, 34.30, 24.40, 12.20, 9.90 and 11.80% to 12.23, 3.72, 31.55, 22.43, 11.15, 9.12 and 11.28%, respectively. Meanwhile, nitrogen retention and CP increased from 58.40 and 8.40% to 63.64 and 11.29%, respectively. ME content of treated was better than untreated JWM (1774 vs. 2599 kkal/kg). Trichoderma viride treatment improved JWM’s amino acids profile. In conclusion, fermentation by 7% Trichoderma viride at 5 day incubation period was the best treatment for improving JWM’s nutrient quality.
  Harnentis , Yetti Marlida , Yose Rizal and Maria Endo Mahata
  Eleven isolates of thermophilic bacteria were isolated from hot spring and collected from West Sumatera of Indonesia. The bacteri isolated were grown on medium agar containing locust bean gum at 60°C for 48 hours to examine their mannanase production by using Congo red test. It was found that four isolates showed positive results with clear zone around the cultures. All isolates were evaluated their mannanase activity by growing in liquid medium supplemented with 0.5% locust bean gum as mannan source. It was found that isolate SM-1.4 displayed that highest enzyme activity of 119,44 U/ml and specific activity of 19.55 U/mg protein. The optimal growth conditions were at 60°C, pH 7 and 24 hours of incubation. According to the morphological studies, the isolate SM-1.4 was primarily identified as the genus Bacillus. The bacteria was gram negative and has endospore.
  Nuraini , Maria Endo Mahata and Nirwansyah
  An experiment was conducted with 200 unsexed broilers of 3 days old of the Arbor Acres strain to evaluate utilization of cocoa pods fermented by Phanerochaete chrysosporium and Monascus purpureus in the diet on the performance of broiler. This study involved a Completely Randomized Design (CRD) with 4 treatments (0, 5, 10 and 15% of fermented product in the diets) and 5 replicates per treatment. Diets were iso nitrogenous (22% crude protein) and iso caloric (3000 kcal/kg diet). Measured variables were performances (feed consumption weight gain, feed conversion, carcass percentage and total cholesterol). Increasing cocoa pod fermented levels in the diets increased feed consumption, weight gain and carcass percentage but decreased (p<0.01) feed conversion and blood cholesterol). In conclusion, up to 15% of cocoa pod fermented by Phanerochaete chrysosporium and Monascus purpureus could be included for broiler diet to increase performances and decreased 35.65% cholesterol level.
  Yose Rizal , Nuraini , Mirnawati and Maria Endo Mahata
  An experiment has been conducted to compare the nutrient contents and nutritional values of palm kernel cake (PKC) from different fermentation processes. This experiment was designed in a Completely Randomized Design (CRD) with six treatments and five replicates. Treatments were A = unfermented PKC (control), B = fermented PKC with Neurospora crassa, C = fermented PKC with Penicillium sp., D = fermented PKC with Trichoderma harzianum, E = fermented PKC with Aspergillus niger and F = fermented PKC with Aspergillus niger+humic acid. Measured variables were nutrient contents (crude protein, crude fiber and ether extract) and nutritional values (nitrogen retention, crude fiber digestibility and metabolizable energy) of fermented and unfermented PKCc. Results of experiment indicated that the crude fiber content of fermented PKCs was very significantly lower (p<0.01) than that of control, but there was no difference (p>0.05) among the fermented PKCs. Fermentation with different fungi increased (p<0.05) the crude protein content of PKCs when it was compared with control. The crude protein content of Aspergillus niger and Aspergillus niger+humic acid fermented PKCs were significantly higher (p<0.05) than that of Trichoderma harzianum, Neurospora crassa, or Penicillium sp., fermented PKCs or control. The ether extract content of PKC was very significantly affected (p<0.01) by treatments. Aspergillus niger+humic acid, Neurospora crassa and Aspergillus niger fermented PKCs possessed the lower ether extract content (p<0.05) than that of Penicillium sp., or Trichoderma harzianum fermented PKCs, or control. The crude fiber digestibility of Aspergillus niger or Aspergillus niger+humic acid fermented PKCs was very significantly higher (p<0.01) than that of Penicillium sp., Trichoderma harzianum, Neurospora crassa fermented PKCs, or control. The nitrogen retention of Aspergillus niger+humic acid fermented PKC was significantly higher (p<0.05) than that of Penicillium sp., Neurospora crassa fermented PKCs, or control, but was not differed (p>0.05) from that of Trichoderma harzianum or Aspergillus niger fermented PKCs. The fermentation with different fungi improved (p<0.05) the metabolizable energy content of PKC. Thus, the improvement of the nutrient contents and nutritional values of Aspergillus niger fermented PKC was better than the other treatments.
  Yose Rizal , Maria Endo Mahata , Guoyao Wu , Hapris Maryudi and Mira Andriani
  Two experiments had been conducted to improve the nutrient quality of carrot and fruits (apple, mango, avocado, orange, melon and tree-potato) juice waste mixture. Experiment 1: to determine effects of rice-hull ash filtrate level and soaking length on nutrient contents of this juice waste mixture. A 3 x 4 factorial experiment in a completely randomized design with 3 replicates was performed. First factor was rice-hull ash filtrate level (10, 20 and 30%) and second factor was soaking length (0, 24, 48 and 72 h). Measured variables were: crude fiber, crude protein and ether extract contents. Experiment 2: to compare treated vs. untreated juice wastes mixture numerically. Nutrient contents (crude fiber, crude protein and ether extract), anti-nutrition contents (phytate and tannin) and nutritive values (amino acids profile, nitrogen retention and metabolizable energy) of treated juice waste mixture were compared with untreated one. Results of experiment 1: crude fiber and ether extract reduced (p<0.01) by soaking in 20% rice-hull ash filtrate for 72 h, while crude protein increased (p<0.01). In experiment 2, crude fiber and ether extract decreased from 17.10 to 12.70% and 6.18 to 5.50%, respectively. Crude protein and phytate increased from 9.58 to 12.22% and 0.84 to 1.28%, respectively. Tannin content did not differ (1.60 vs. 1.59%). Amino acid contents were improved. Nitrogen retention and metabolizable energy were augmented from 59.99 to 67.57% and from 1744 to 2717 kcal/kg, respectively. In conclusion, carrot and fruit juice wastes mixture soaking in 20% rice-hull ash filtrate for 72 h improved its nutrient quality.
  Adrizal , Yan Heryandi , Robi Amizar and Maria Endo Mahata
  Background and Objective: The utilization of pineapple waste as poultry feed is limited due to the high crude fiber and water contents and the low protein content. The crude fiber content in pineapple waste can be reduced by simple fermentation using local, naturally beneficial microorganism solutions, particularly cellulolytic microorganisms. Local microorganisms can be obtained from rice waste, bamboo sprouts, banana corms, mixed fruit waste and mixed vegetable waste. Thus, this study evaluated the nutrient content of pineapple waste, particularly crude fiber content, using submerged fermentation and different local microorganism solutions from rice waste, bamboo sprouts, banana corms, mixed fruit waste and mixed vegetable waste. Materials and Methods: This experiment was performed in a completely randomized design with two factors. The first factor was different local microorganism solutions and the second factor was the fermentation duration (1, 2 and 3 weeks). Each treatment was replicated twice. Total water, dry matter, crude protein, crude fiber, soluble protein and total sugar contents, as well as the cellulase activity were measured. Results: The results showed an association (p<0.05) between the different local microorganism solutions and the fermentation durations on the crude fiber, total sugar and soluble protein contents of the pineapple waste fermentation product. Cellulose activity in pineapple waste was significantly affected by the different local microorganism solutions and the fermentation duration and the different local microorganism solutions significantly affected the crude protein, water and dry matter contents (p<0.05). Conclusion: Higher total water and lower total dry matter contents of the pineapple waste fermentation product were detected using the local microorganism solution from bamboo sprouts and a higher total crude protein content were detected using the local microorganism solutions from rice waste, bamboo sprouts, mixed fruit waste and mixed vegetable waste, further a lower total crude fiber content was detected using the local microorganism solution from bamboo sprouts with a fermentation duration of 1, 2 and 3 weeks. A higher total sugar content was detected using the local microorganism solution from rice waste and a higher soluble protein content was detected using the local microorganism solution from rice waste with a fermentation duration of 1 week, using that from mixed fruit waste with a fermentation duration of 2 weeks and using that from bamboo sprouts with a fermentation duration of 3 weeks. A higher cellulase activity during pineapple waste fermentation was detected using the mixed local microorganism solutions from vegetable and mixed fruit waste. The local microorganism solution from bamboo sprouts and a fermentation duration of 1 week was the best combination for lowering the crude fiber content of pineapple waste.
  Zurmiati , Wizna , M. Hafil Abbas and Maria Endo Mahata
  Background and Objective: Mannanase is a decomposer of the compound mannan, which is produced by many microorganisms including bacteria. This enzyme can be utilized in various industrial processes, such as in the animal feed industry to increase the nutritional value of the feed. This study aims to optimize the production and function of the mannanase enzyme, which is produced by Bacillus amyloliquefaciens on a coconut waste substrate. Methodology: This study was designed in the form of laboratory experiments described herein and repeated 3 times. The substrate dose, incubation time, optimum pH and temperature of the mannanase enzyme were measured. Results: The results of this study showed that the best conditions were a substrate ratio of 80% coconut waste to 20% tofu waste, a 48 h incubation time, a pH of 6.5 and a temperature of 40°C yielding a mannanase enzymatic activity of 5.13 U mL–1. When locust bean gum was added to the substrate composed of coconut and tofu waste, the best conditions were a dose of 0.6%, a 48 h incubation time, a pH = 6.5 and a temperature of 40°C yielding a 5.92 U mL–1 mannanase enzymatic activity. Conclusion: It is concluded that the activity of the mannanase enzyme with the substrate composed of coconut and tofu waste is not much different from that with the substrate containing locust bean gum, with the difference being only 13.34%.
  Wingki Ari Angga , Yose Rizal , Maria Endo Mahata , Ahadiya Yuniza and Reni Mayerni
  Background and Objective: West Sumatra is the third largest tea-producing area in Indonesia. Tea plantations in this area produce top quality leaves that can be marketed both domestically and internationally. To maintain a high level of tea leaf productivity, plants should be pruned every 3 years using a rotation system that involves monthly prunings. These prunings produce waste tea leaves that can serve as alternative feed resource for poultry as they have good nutritional value. Tea leaves contain high concentrations of antioxidants, such as polyphenols and policosanol as well as minerals and vitamins, which are known to improve poultry health. Unfortunately, tea leaves also contain high levels of tannins and crude fiber-components known to be detrimental to poultry. This study was designed to evaluate the potential of waste tea leaves (Camellia sinensis) as poultry feed in West Sumatra by reducing their tannin content through immersion in fresh and hot water. Materials and Methods: This research consisted of two phases. The first phase was a survey of the potential of waste tea leaves as poultry feed through interviews and measurement of waste tea leaf production. The second phase was to experimentally process waste tea leaves through immersion in fresh and hot water. Variables measured during phase 1 included the size of tea plantations, ownerships, tea varieties produced, waste tea leaf production and estimated potential for poultry feed. The experiment conducted in phase 2 was performed using a completely randomized design involving 2×4 factorial arrangement of treatments with 4 replicates. The first experimental variable was water temperature (room temperature or 80°C). The second experimental variable was based on immersion at 6, 12, 18 or 24 h. Response variables measured included change in tannin content, dry matter (DM), organic matter (OM), crude protein (CP) and crude fiber (CF). Results: The results of the first phase indicated that the area of tea plantations in West Sumatra was 4,246.6 ha, ownership consisted of small holders (2,172 ha), the government (604.58 ha) and a private company (1,470 ha), total waste tea leaf production was 25,208.28 t/year, tea varieties or clones were Camellia sinensis assamica TRI 2024 and assamica TRI 2025 and waste tea leaves had the potential to feed 4,201,380,000 laying hens. The results of the second phase indicated that there was an interaction between water temperature and immersion duration on tannin reduction (p<0.05). Water temperature significantly influenced (p<0.01) reductions in OM and CP content and significantly affected (p<0.05) CF augmentation. Immersion duration significantly affected (p<0.05) DM reductions and highly significantly influenced (p<0.01) reductions in OM and CP. Conclusion: Waste tea leaves can be immersed in hot water (80°C) for 12 h to reduce their tannin content without affecting their protein content.
 
 
 
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