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We have earlier reported on the expression of Sendai virus HN gene in BHK-21 cells (Ansari and McQueen 1999). As expression gives no idea about the functional activity of the protein therefore we are reporting here on the functional activities of the expressed HN protein. HN is a multi functional molecule having hemagglutinin activity and neuraminidase activity. We tested both the activities separately and found both the activities in the in vitro expressed HN protein. The results confirmed that the HN gene not only is synthesized but is functionally active too.
Twenty isolates of Pseudomonas sp., were tested for the control of soilborne root-infecting fungi like Macrophomina phaseolina, Fusarium solani and Rhizoctonia solani under laboratory and greenhouse conditions. In dual culture plate assay, strain 78 inhibited all the test fungi. Other Pseudomonas sp., failed to check the radial growth of M. phaseolina whereas isolates 51 and 82 significantly inhibited radial growth of F. solani and R. solani. Three isolates of Pseudomonas sp., (51, 78 and 82) when used as seed dressing or as soil drench showed substantial reduction in root-rot infection caused by M. phaseolina, F. solani and R. solani in mungbean. Strain 78 was found to be most effective in the control of root-infecting fungi. Of the root-infecting fungi, R. solani was most susceptible to Pseudomonas sp.
