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Fatty acid analysis in cotton seedlings following waterlogging showed changes in both their levels and relative composition. This treatment caused a marked increase in the amount of esterified fatty acids. Waterlogging also enhanced the synthesis of a set of low molecular weight compounds and suppressed the synthesis of all the normal fatty acids. A significant increase in free fatty acids was also observed.
The results of fatty acids analysis suggested that greater unsaturation occurred with increasing germination time. High temperature had a marked effect on fatty acid composition and caused significant changes especially in neutral lipid and glycollpid fatty acids. Small changes were also observed in phophollpid fatty acids. A large increase in free fatty acid was also found, since free fatty acids are known to be damaging to cells.