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Articles by Manoj Prasad
Total Records ( 1 ) for Manoj Prasad
  Manoj Kumar , Meetu Gupta , Divya Shrivastava , Manoj Prasad , U.S. Prasad and Neera Bhalla Sarin
  Genetic relatedness among Indian litchi (Litchi chinensis Sonn.) cultivars was investigated using RAPD (random amplified polymorphic DNA) markers. Fourteen RAPD primers which produced consistent profiles were chosen, resulting in amplification of 77 reproducible polymorphic bands. The RAPD analysis produced an average of 15.8% polymorphic and 0.10% monomorphic markers. With the help of RAPD marker system we were able to classify all the accessions into different groups despite of their same or different geographical origins and climatic adaptations. The polymorphism information content scores were calculated for each of the 77 RAPD polymorphic fragments. Unweighted pair-group method with arithmetic average (UPGMA) dendrograms using Jaccard`s coefficients reflected no clear cut variation or grouping based on either morphology or climatic adaptation. However, dendrogram showed that 27 accessions of Indian litchi could be classified into groups when the similarity coefficients were shown in a range of 0.11 to 0.47. Two accessions [Chinarose (B) and Late Bedana (B)] were found to be genetically significantly distant from the other accessions using RAPD markers. The information obtained from the present study could be of practical use in mapping the litchi genome as well as for classical breeding.
 
 
 
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