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Articles by Manlio Silvestre Fernandes
Total Records ( 2 ) for Manlio Silvestre Fernandes
  Fabiola de S.R.G. Garrido , Rodrigo Grazinoli Garrido , Carlos Alberto Bucher , Sonia Regina de Souza and Manlio Silvestre Fernandes
  In a growth chamber experiment, the response of two rice varieties (Piaui and IAC-47) to nitrate suppression (24 h) and pulses (72 h) was studied. Nitrate suppression in nutrient solution increased plasma membrane H+-ATPase activity in Piaui plants previously grown under 0.5 mM and IAC-47 under 5.0 mM NO3-N. Under NO3- pulses, IAC-47 presented highest H+-ATPase activity in its plasma membrane, while Piaui showed increases in tonoplast V-H+-ATPase activity. These results suggested that under nitrate pulses IAC-47 increased its ability to take up NO3- from nutrient solution. Piaui plants preferentially retrieves nitrate stored from the vacuoles. Plasmalem protein content in Piaui tonoplast was enhanced particularly in -N plants previously grown under 5.0 mM NO3--N. We suggest that these enzymes (H+-ATPases) could be monitored as markers in nitrogen use efficiency study in rice plants.
  Rodrigo Grazinoli Garrido , Fabiola de S.R.G. Garrido , Sonia Regina de Souza and Manlio Silvestre Fernandes
  In this study, two rice varieties were evaluated: Piaui and IAC-47. Piaui is adapted to Humid Tropic environmental conditions, where there is N deficiency and low light since rice is planted. IAC-47 is an improved variety. This research aimed to characterize physiological and morphological adaptations observed during vegetative growth under low ammonium-N nutrition and low light in both varieties. To analyze it, developmental parameters, soluble sugars content, amino-N, NH4+, total-N and pigments were compared between varieties. The assay was carried out in controlled growth chamber conditions, at 24°C and 12/12 h light/dark periods (200 μE m-2 sec-1). Rice plants were grown in nutrient solution until 26 Days After Germination with a modified Hoagland and Arnon. All plants received two treatments with NH4+-N 0.1 and 1.0 mM, pH 5.5. Under these conditions, Piaui plants presented longer roots, chlorophyll a+b enhances, soluble sugars levels reduction and free amino-N content increases, what would be important for its adaptation to low N and low light environments, found in Humid Tropic rice culture. In spite of this, IAC-47, selected for high nutrient and light availability, presented typical senescence symptoms.
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