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Articles by Mandana Ohadi
Total Records ( 2 ) for Mandana Ohadi
  Mandana Ohadi , Bagher Amir-Heidari , Mohammad Hassan Moshafi , Ali Mirparizi , Mohammadzaman Basir and Gholamreza Dehghan-Noudeh
  In the present study, biosurfactant production by immobilized Bacillus licheniformis PTCC 1320 in alginate beads has been studied. Alginate solution and bacterial suspension were mixed and stirred to get a uniform mixture. The slurry was taken into a sterile syringe and added drop wise into a stirring calcium chloride solution from a 5 cm height to form spherical beads. The beads were kept under agitation for about 2 h to harden and were then washed. Equal numbers of beads were charged to 250 mL erlenmeyer flasks each containing 50 mL of nutrient broth medium. Beads containing B. licheniformis placed in the nutrient broth medium were incubated in a shaker incubator at 30°C. Samples were collected at 24 h intervals and the parameters of biosurfactant production, such as surface tension, emulsification activity and foam production were studied. Results showed that B. licheniformis entrapped in calcium alginate beads is able to preserve its viability and produce biosurfactant as a secondary metabolite. Maximum biosurfactant production was achieved during the first 24 h.
  Zahra Shahba , Amin Baghizadeh , Mehdi Yousefi and Mandana Ohadi
  Of the effects of salinity stress is the production of Reactive Oxygen Species (ROS) and creation of oxidative stress, where ROS rapidly attack all kinds of biomolecules such as nucleic acids, proteins, lipids and amino acids and lead to abnormal metabolic function. Salicylic acid reduces the effects of oxidative stress. In this research, first, seeds were cultured in the pots containing perlite and then they were put in a growth room under controlled conditions at 27±2 and 23±2°C in light (16 h) and dark (8 h) conditions, respectively with luminous intensity of 15 klx and moisture of 75%. Five concentrations of NaCl including 0, 25, 50, 75 and 100 nM and 4 concentrations of salicylic acid including 0, 1.5 and 1.5 mM were used. Then, peroxidation rates of lipids, ascorbic acid and dehydroascorbic acids were measured to study the effects of oxidative stress. High salinity significantly increased malondealdehyde rate. At different salinity levels, a decrease in other aldehydes was found and salicylic acid decreased its effect in most treatments. Content of ascorbic and dehydroascorbic acids increased by salinity and its salicylic acid was reduced.
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