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Articles by Maizura Mat Saad
Total Records ( 1 ) for Maizura Mat Saad
  Maizirwan Mel , Maizura Mat Saad , Yumi Zuhanis Hasyun Hashim and Mohamad Ramlan Mohamed Salleh
  Media optimization of RC1 hybridoma cell culture for monoclonal antibody production was carried out in T-Flask experiment. The three identified important variables to affect the cell viability were studied. By using Central Composite Design of Response Surface Methodology (STATISTICA v 6.1) has shown that cell viability was mainly affected by glutamine, serum and NaCO3 concentration, respectively. Among the 16 runs tested, Run 11 indicated the best viability of the cell (>80% for five days). The critical values were obtained at 13.5, 1.68 and 0.87% for serum, glutamine and NaCO3, respectively. These data were very significant where the p-values obtained for glutamine, serum and NaCO3 were 0.000069, 0.003968 and 0.342151 (R2 = 0.95476 and R Adj. = 0.88691), respectively.
 
 
 
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