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Articles by Mairizal
Total Records ( 2 ) for Mairizal
  Mairizal , Yetti Marlida , Mirzah and Fahmida Manin
  Background and Objective: Mannanase is a mannan-degrading enzyme that can be produced by microorganisms such as bacteria. Mannanase is widely used in the animal feed industry with the aim of improving the quality of feed ingredients containing high levels of mannan, such as palm kernel meal. This study aimed to isolate, characterize and produce mannanase from bacteria found in the hindgut of termites. Materials and Methods: Bacteria were isolated by culturing on solid media containing 0.5% locust bean gum for 24 h at 30°C. The mannanolytic index was then measured by the addition of 2% congo red, which was then washed with 1 M NaCl. Mannanase activity was measured by the dinitrosalicylic acid method, while the protein content was measured by the Bradford method. Results: A total of 8 isolates were obtained. The 3 isolates with the highest mannanolytic indices were selected (isolates V9, AZ and AD). Isolate V9 exhibited the highest mannanase activity of 29.50 U mL–1 after 88 h of incubation with a specific activity of 110.32 U mg–1. The activity of the mannanase was optimal at pH 8 and at an incubation temperature of 50°C. Based on 16S-DNA analysis, isolate V9 was identified as Bacillus cereus. Conclusion: Bacillus cereus, which is isolated from the hindgut of termites, has fairly high mannanase activity and has the potential to hydrolyse mannan in feed ingredients such as palm kernel meal.
  Mairizal , Fahmida Manin and Ella Hendalia
  Background and Objective: Palm kernel meal is a byproduct of processing palm oil that can be used as animal feed. Palm kernel meal contains mannan in sufficiently high quantities that its use is limited to poultry. Enzymatic hydrolysis of palm kernel cake with the enzyme mannanase produces mannose and mannooligosaccharides (MOS), which can act as prebiotics. Prebiotics are used for the growth of probiotic microbes in the digestive tract of broilers. Probiotics are living microorganisms that are given in sufficient quantities and provide beneficial effects to the health of livestock while being able to increase livestock production. This study aimed to examine the effect of probiotics and palm kernel meal hydrolyzed with mannanase enzymes on broiler growth performance. Materials and Methods: This study used a completely randomized 3×6 factorial design with 3 replicates. The first factor was 3 doses of probiotics through the drinking water (0, 1 and 2%) and the second factor was 6 ration levels of palm kernel cake after hydrolysis with mannanase enzymes (0, 10, 15, 20, 25 and 30%). The variables observed included feed consumption, body weight gain, feed conversion ratio and mortality. Results: The supply of up to 30% palm kernel meal after enzymatic hydrolysis in broiler rations did not significantly influence (p>0.05) feed consumption, body weight gain and feed conversion ratio. Probiotic administration to the level of 2% through the drinking water also showed no significant effect (p>0.05) on feed consumption, body weight gain and feed conversion ratio but showed a significant effect (p<0.05) on mortality. The provision of 1 and 2% probiotics can reduce mortality compared to feed without probiotics. The interaction between the administration of palm kernel meal after enzyme hydrolysis and the administration of probiotics showed no significant effect (p>0.05) on all the observed parameters. Conclusion: Based on these results, it can be concluded that the administration of up to 30% palm kernel meal after enzyme hydrolysis has no negative effects in broiler rations. Probiotic administration to the level of 2% also had no negative effects on broiler growth performance and did not reduce survival. There was no interaction between the palm kernel meal and probiotics on broiler growth performance.
 
 
 
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