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Articles by Mahmoud Abd El-Mongy
Total Records ( 2 ) for Mahmoud Abd El-Mongy
  Ragaa Abd El Fatah Hamouda , Mahmoud Abd El-Mongy and Kamel Fatehy Eid
  Background and Objective: Silver nanoparticles were synthesized via chemical reduction and using stabilizers agents. This study aimed to compare between biosynthesis of silver nanoparticles (AgNPs) by marine alga Ulva fasciata (U. fasciata) as reducing and stabilizing agents with biosynthesis of AgNPs by marine alga U. fasciata as reducing agents and sodium dodecyl sulfate as stabilizer against bacterial activities. Materials and Methods: In this study macro green alga (Ulva fasciata) aqueous extracts using as reducing agents to reduce Ag ions to Ag0 and sodium dodecyl sulfate (SDS) as stabilizer. Ultra violet-visible spectroscopy, transmission electron microscope (TEM), scanning electron microscopy (SEM), fourier transform infrared spectroscopy, energy dispersive X-ray spectroscopy (EDX). Zeta potential and zeta sizer were used to characterize green synthesis silver nanoparticles (AgNPs). Results: Biosynthesis of AgNPs showed broad surface plasmon resonance peak at 446, 428 and 426 nm with 1 h and 1 and 2 weeks that reflects the stability of nanoparticles until 2 weeks. Transmission electron microscopy study proved that the shape is spherical and average size was ranged from 9-20 nm. Fourier transform infrared spectroscopy analysis (FTIR) indicated evidence that pretence of amide band of protein as possible reducing agents. Zeta potential was at -30, zeta sizer indicated that the size range around 53.5 nm. Biosynthesis AgNPs had antibacterial activities against Escherichia coli ATTCC 8739, Proteus mirabilis ATTCC 9240, Micrococcus leutus and Kocuria varians. Conclusion: The biosynthesis of silver nanoparticles using U. fasciata aqueous extracts as reducing agents and sodium dodecyl sulfate (SDS) as stabilizer had highest inhibition activity against both Gram-negative and Gram-positive pathogenic bacteria more than silver nanoparticles biosynthesis by U. fasciata aqueous extracts as reducing and stabilizing agents and also AgNO3 as a bulk.
  Mahmoud Abd El-Mongy , Ghada Mohammed Abd-El-Moneam , Amgad Ahmed Moawad and A.B. Abeer Mohammed
  Background and Objective: Escherichia coli (E. coli) strains causing systemic disease in poultry (avian colibacillosis) are termed avian pathogenic E. coli (APEC). Colibacillosis is a disease of severe economic significance to all poultry producers worldwide and is characterized by a diverse array of lesions. Escherichia coli that cause infections usually possess one or more virulence properties that may help in establishment of the infection. The aim of this study was to investigate the virulence genes in E. coli isolated from broiler chickens. Methodology: A total number of 125 chicken samples from apparently healthy broiler chickens (25 and 15), diseased broiler chickens (25 and 15) and freshly dead ones (25 and 20) were collected in winter (from December, 2014 to February, 2015) and summer (from June, 2015 to August, 2016), respectively from Kafr El-sheikh Governorate. Results: In winter season, E. coli was recovered from 43 broiler chickens with an incidence of 57.3% and the incidence of E. coli in apparently healthy broiler chickens was 32%, diseased broiler chickens 64% and in freshly dead ones 76% while in summer season E. coli was recovered from 21 broiler chickens with an incidence 42% represented 26.6% in apparently healthy, 40% in diseased chickens and 55% in freshly dead one. The serogroups of E. coli that obtained by serological identification were O78, O1, O26, O2, O127, O91 and O153. The results obtained by multiplex PCR reported that eaeA (intimin E. coli attaching and effacing) gene detected in O2, O26, O1 and O153, ompA (outer membrane protein) gene detected in all E. coli serogroups that isolated O2, O26, O78, O127, O1 and O91 except O153. Stx1 gene detected in O2, O26, O78 and O91. Stx2 gene detected in O78, O127 and O91. Conclusion: The present study showed a higher percentage of E. coli isolates carrying at least one virulence gene.
 
 
 
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