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Articles by Mahmoud El-Sayed Ottai
Total Records ( 1 ) for Mahmoud El-Sayed Ottai
  Sahar Youssef Al-Okbi , Adel Gabr Abdel-Razek , Shaimaa Elsayed Mohammed and Mahmoud El-Sayed Ottai
  Background and Objective: Searching non-traditional sources of edible oil is crucial in Egypt. Roselle (Hibiscus sabdariffa L.) is a vital medicinal plant in Egypt, where all its parts are utilized except the seeds that are completely discarded. The aim of the present research was to study the possibility of consuming roselle seed oil (RSO) as edible oil. Materials and Methods: The composition of RSO from fatty acids, total phenolic, flavonoids and tocochromanols was assessed. In vitro antioxidant and ABTS scavenging activity and the oxidative stability of RSO were evaluated. Oral acute lethal toxicity test of RSO was studied in mice to assess its safety. Two experiments were carried out on rats, in the first experiment two balanced diets were fed to two groups of rats one contained 10% RSO while the other contained the same percentage of sunflower oil as reference oil for 4 weeks. At the end of the experiment, plasma lipid profile, malondialdehyde (MDA) and liver and kidney functions were assessed. In the second experiment, dyslipidemia was induced in rats then rats were fed either 10% RSO or 10% sunflower oil diet. At the end of the second experiment plasma glucose, lipid profile, interlukin 6 and MDA were assessed. Data were statistically analyzed using one-way analysis of variance ANOVA followed by Duncan’s test. Results: The RSO showed to possess high safety and in vitro antioxidant activity. Major fatty acids were linoleic, oleic and palmitic. Total tocopherols and tocotrienols were 96.2 and 3.48 mg/100 g oil, respectively. Total phenolic and flavonoids contents were 56.31mg GAE and 4.99 mg catechin g–1 oil, respectively. Induction period of oxidative stability of RSO was 24.88 h. All assessed parameters of first rats experiment showed insignificant changes when rats fed on RSO diet was compared to those fed on sunflower oil diet. In the second experiment, significant improvements in all parameters were noticed when dyslipidemic rats were fed on either RSO or sunflower diet with superiority to RSO concerning MDA and interlukin 6. Conclusion: The RSO has high safety, oxidative stability and antioxidant activity and cardiovascular remedial effect. It is proposed that RSO is suitable as edible oil for human consumption.
 
 
 
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