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Articles by Mahmood Salehi
Total Records ( 2 ) for Mahmood Salehi
  Reza Ghanbarpour and Mahmood Salehi
  Problem statement: Omphalitis is one of the most common causes of mortality in chicks during the first week after hatching. Escherichia coli strains are the most common isolated bacteria from omphalitis cases of chickens. Bacterial colonization in the host cells surfaces is a critical first step in the pathogenesis of avian pathogenic Escherichia coli isolates. Thus the current study was undertaken to determine the presence and prevalence of several adhesin-encoding genes in E. coli isolates from omphalitis of chicks. Approach: One hundred four E. coli isolates were recovered from omphalitis cases and were identified by standard biochemical tests. The omphalitis-derived isolates were examined for the presence of fimbrial and non-fimbrial adhesin-encoding genes by PCR technique. Results: Most (93.26%) of the E. coli isolates exhibited at least one of the examined adhesin-encoding genes. None of the isolates contained the afaI B-C, afa E-VIII and f17A genes. The two most prevalent genes were crl (87.50%) and fimH (77.88%). P (papC) and S (sfa) fimbriae encoding genes were detected in 8 (7.69%) and 5 (4.80%) isolates respectively. Seven combination patterns of the adhesin-encoding genes were detected. In 83 (79.80%) isolates combinations of 2-4 genes were detected. The gene combinations of crl-fimH and fimH-papC were the two most prevalent patterns respectively. Fourteen (13.46%) isolates showed crl gene alone and 7 (6.73%) isolates were negative for examined genes. Conclusion: The current study showed that some of the adhesin-encoding genes are more prevalent in E. coli isolates from omphalitis of chicks but, E. coli isolates may be expressing still unknown adhesins that could have a role in the pathogenicity of omphalitis-derived isolates.
  Mahmood Salehi and Reza Ghanbarpour
  Problem statement: Escherichia coli isolates are the most common bacterial agents of salpingitis in commercial layer hens. The objectives of this study were to determine the phylogenetic groups/subgroups and antibiotic resistance of E. coli isolates from salpingitis cases in commercial layer hens farms in southeast of Iran. Approach: One hundred twenty one E. coli isolates from oviducts of layer hens with salpingitis were examined to determine their O-serogroup, phylogenetic group/subgroup and antibiotic resistance pattern. Results: O-serogroup determination test showed that 47 (38.84%) isolates were typeable and belonged to eleven different O serogroups including: O1, O2, O6, O8, O15, O20, O25, O36, O78, O86 and O111 and 71 (58.67%) isolates were O-nontypeable. Three most prevalent serogroup were O78, O2 and O1 respectively. PCR assays showed that the isolates fall into four phylogenetic groups A (41.32%), D (33.88%), B2 (14.87%) and B1 (9.91%). Ninety nine (81.81%) isolates fell into six phylogenetic subgroups including: A0 (17.35%), A1 (23.96%), B22 (5.78%), B23 (9.09%), D1 (13.22%) and D2 (20.66%). All of the E. coli isolates from O78 serogroup belonged to A phylo-group whereas the O2 isolates mostly fell into D group. The maximum antibiotic resistance was against tetracycline (100%) and minimum resistance was against linco-spectin (37.19%). Twenty four isolates (19.83%) were resistant to all of the examined antibiotics. Twenty three different patterns of multiple drug resistance were observed, out of which N-Te-Sxt-Fm-Nfx-C and N-Te-Sxt-Gm-Fm-Nfx-C-Ls was the two most frequent patterns respectively. The resistant isolates were found in all of the phylogenetic groups and or sub groups. Conclusion: There are similarities between salpingitis derived E. coli strains and other avian pathogenic E. coli isolates in phylogroups, O-serogroups and antibio-resistance patterns.
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