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Articles by Mahmood Maziah
Total Records ( 4 ) for Mahmood Maziah
  Siamak Shirani , Meon Sariah , Wahab Zakaria and Mahmood Maziah
  Problem statement: The effect of cytokinins on scalp induction from in vitro propagating shoot tips of different banana (Musa spp. AAA and AAB) cultivars was studied. Approach: Proliferation rate increased with an increasing concentration of Benzyl Amino Purine (BAP) up to 22.2 ΜM while, lower level of BAP (11.1 ΜM) increased scalp induction. Results: Kinetin induced a lower number of shoots than that obtained with similar levels of BAP and caused an increase in scalp induction rate at higher concentration (22.2 ΜM). Shoot proliferation in ‘Rastali’(AAB), ‘Berangan Intan’ and ‘Berangan’(AAA) significantly increased with increasing Thidiazuron (TDZ) concentration from 3.17, 2.17 and 3.33 shoots at 0.5 ΜM to 14.17, 6.22 and 6.17 shoots at 5 ΜM respectively, as concentrations above 5 ΜM for all cultivars were inhibitor. Conclusion/Recommendations: The highest ratio of scalp formation (8.89) was recorded in ‘Rastali’ at the highest concentration of TDZ (7.5 ΜM), but in ‘Berangan Intan’ and ‘Berangan’ (AAA) TDZ increased scalp induction rate from 0.00 and 0.43 at 0.5 ΜM to 4.22 and 2.67 at 5 ΜM respectively before falling to 2.11 for both at 7.5 ΜM. BAP at 22.2 ΜM was considered optimal for shoot proliferation as well as shoot elongation from excised scalps of banana cultivars.
  Suat Hian Tan , Radzali Musa , Arbakariya Ariff and Mahmood Maziah
  Problem statement: Considering pegaga medicinal properties and over-exploitation, the requirement for a tissue culture technique as an alternative production system was crucial. Approach: Investigation of cell suspension culture response to different plant growth regulators (PRGs) for flavonoid production from elite cell line was carried out. Callus cultures were initiated from the leaf explants of Centella asiatica on Murashige and Skoog (MS) medium containing B5 vitamins and 30 g L-1 sucrose supplemented with different concentrations (0.5-2.5 mg L-1) of 2,4-D, NAA, Dicamba, Picloram and IBA supplied singly and in combination with different concentrations (0.5-1.5 mg L-1) of kinetin, BAP and TDZ. Results: Callus induction was observed for all the PGRs tested. The highest callus induction frequency (86.67%) was observed in MS medium containing 2.0 mg L-1 2,4-D while the combination of 2.0 mg L-1 2,4-D and 1 mg L-1 kinetin in MS medium gave the highest biomass yield (0.27 g dry weight culture-1). This combination was also found to be best for callus proliferation for all the accessions investigated. Among the four accessions tested, UPM03 was found to have the highest biomass yield (0.041 g DW culture-1) and hydrolysed flavonoid content (10.75 mg g-1 DW) after the 12th day of culture. The flavonoids present in the four accessions were quercetin, kaempherol, luteolin and rutin based on High Performance Liquid Chromatography (HPLC) analysis. These results indicated that C. asiatica accession UPM03 was the potential elite cell line in mass production of flavonoid, especially luteolin. Coclusions/Recommendations: In the establishment of cell suspension culture, 2 mg L-1 2,4-D and 1 mg L-1 kinetin were the best PGRs in supporting the cell growth and flavonoid production. This is the first report on the use of PRGs on the establishment of cell suspension cultures in flavonoid production of C. asiatica.
  Nyuk Ling Ma , Thye San Cha , Mahmood Maziah and Ahmad Aziz
  Aquatic plant posses a very interesting stress tolerance mechanism compared to terrestrial plants. However, lack of aquatic mutants available is a hindrance to explore the cellular mechanism that governs various adaptations between these two types of plants. Therefore, the aim of this study is to provide the optimum parameters for the genetic transformation of native aquatic plant in tropical country, Aglaonema simplex, by using stem and callus tissue. Explants were bombarded with various sizes of gold particles coated with 35 sec promoter constructed into 35 sec-sGFP-TYG-nos (puc18) plasmid harbouring green fluorescent protein. High transient expression was obtained when the tissues were bombarded twice by gold particles coated with spermidine under acceleration pressure of 1100-1350 psi and with the vacuum pressure set to 24 Hg. The calli were found susceptible to high pressure impact, showing better result at a longer bombardment distance (12 cm) of stopping screen to explants by the use of gold particle of smaller size (1 μm). In contrast, the optimal bombardment distance shown by stem tissues was achieved at a 6 cm distance by the use of gold particle of 1.6 μm. PCR and PCR-southern blot analyses exhibited that the gene was integrated into the genome of transformants and this indicates that the protocol can be used for genetic engineering purpose in the future.
  Nwe Nwe Htwe , Ho Chai Ling , Faridah Qamaruz Zaman and Mahmood Maziah
  Rice is one of the most important cereal crops with great potential for biotechnology progress. In transformation method, antibiotic resistance genes are routinely used as powerful markers for selecting transformed cells from surrounding non-transformed cells. In this study, the toxicity level of hygromycin was optimized for two selected mutant rice lines, MR219 line 4 and line 9. The mature embryos were isolated and cultured on an MS medium with different hygromycin concentrations (0, 20, 40, 60, 80 and 100 mg L-1). Evidently, above 60 mg L-1 was effective for callus formation and observed completely dead. Further there were tested for specific concentration (0-60). Although, 21.28% calli survived on the medium containing 45 mg L-1 hygromycin, it seemed suitable for the identification of putative transformants. These findings indicated that a system for rice transformation in a relatively high frequency and the transgenes are stably expressed in the transgenic plants. Green shoots were regenerated from the explant under hygromycin stress. RT-PCR using hptII and gus sequence specific primer and Southern blot analysis were used to confirm the presence of the transgene and to determine the transformation efficiency for their stable integration in regenerated plants. This study demonstrated that the hygromycin resistance can be used as an effective marker for rice transformation.
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