Asian Science Citation Index is committed to provide an authoritative, trusted and significant information by the coverage of the most important and influential journals to meet the needs of the global scientific community.  
ASCI Database
308-Lasani Town,
Sargodha Road,
Faisalabad, Pakistan
Fax: +92-41-8815544
Contact Via Web
Suggest a Journal
 
Articles by Maged Ahmed AL-Garadi
Total Records ( 2 ) for Maged Ahmed AL-Garadi
  Maged Ahmed AL-Garadi , S. Khairani-Bejo , Z. Zunita and A.R. Omar
  Isolation of Brucella melitensis is the standard gold of identification and confirmation of animal brucellosis. However in Malaysia, Brucella sp., infection of goat was increasing recently and there is no evidence for diagnosis of the serover of Brucella sp., that cause the disease in goat population except the detection of serological methods. Isolation and identification of Brucella melitensis have been done by bacteriological methods in addition to conventional Polymerase Chain Reaction (PCR) and Real-time PCR for detection of Brucella melitensis from samples collected from vaginal swabs on suspected farm. In conclusion, four isolate have been got out of 300 vaginal samples and all isolate is belong to Brucella melitensis server 1. The Real-time PCR is the easy and save method for confirmation of brucellosis in goats population.
  Maged Ahmed AL-Garadi , S. Khairani-Bejo , Z. Zunita and A.R. Omar
  PCR assays have been shown to be a promising option for the diagnosis of brucellosis. However, there is no study conducted in Malaysia to identify the brucellosis in goat’s population. In this study three whole blood samples and sera were collected from goat’s farm in Kedah state Malaysia which was suspected to have brucellosis. Serological and molecular detection of brucellosis have been done including RBPT, CFT, conventional PCR and Real time. The evaluation of each test have been discussed rather than the sensitivity and specificity of the each test which can be used in Malaysia national eradication programs. In conclusion, the combination between the serological test and molecular technique specially real time PCR depend on IS711 region in hypothetical protein is promising and can be reduced to false positive result which can cause heavy economical loss during controlling programs.
 
 
 
Copyright   |   Desclaimer   |    Privacy Policy   |   Browsers   |   Accessibility