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Articles by Magdy M. Youssef
Total Records ( 3 ) for Magdy M. Youssef
  Magdy M. Youssef and Mohammed A. Al-Omair
  In the present study, we report the cloning of L-asparaginase II (asnII) gene from E. coli W3110 into pGEX-2T DNA vector. The L-asparaginase II enzyme (E.C. was overexpressed in E. coli BL21(DE3) and purified to homogeneity 238.4 fold by utilizing chromatography technique on DEAE-Sepharose fast flow, Glutathione S sepharose 4B columns and thrombin. SDS-PAGE of the purified enzyme revealed that has Mr of 40 kDa. In addition, we found that the enzyme can be efficiently immobilized in calcium alginate gelatin composites. The free enzyme has an optimum pH at 7.5 but this optimum pH is shifted to 8.5 for the immobilized enzyme. The optimum temperature, for free and immobilized enzyme were 37 and 50°C, respectively. The immobilized enzyme retained most of its activity at 60°C with high stability compared with the native enzyme when incubated at 60°C for 30 min.
  Mohammed A. Al-Omair and Magdy M. Youssef
  The present study was designed to investigate the effect of selenium (Se) administration (as sodium selenite: 0.5 or 1 mg kg-1 body weight) or/and α-difluromethylornithine (DFMO: 2 mg kg-1 body weight) on the elevated polyamine levels. For this purpose the polyamine (putrescine, spermidine, spermine) levels were elevated in female Swiss albino mice by adding 2% of both L-arginine and L-ornithine to the drinking water for four weeks. The elevated putrescine and spermidine levels were decreased significantly by administration low and high dose of Se. The DFMO abolished the concentrations of both putrescine and spermidine in liver and kidney of the experimental mice. The combination of Se and DFMO normalized the polyamine levels. On the other hand, the spermine level was increased in liver and kidney by administration of Se or/and DFMO. To elucidate the effects of the elevated polyamines on the oxidative enzymes, the activity of glutathione peroxidase (GSH-PX), glutathione reductase (GSH-R) and superoxide dismutase (SOD) in the liver and the kidneys of the experimental mice were determined. The GSH-Px, GSH-R and SOD activities were increased by Se administration compared to the control. Conversely, DFMO produced an inhibition in the activity of antioxidative enzymes. These results suggest that the combination between Se and DFMO appear to be additive chemopreventive effect to reduce the elevated polyamine levels. This combination will protect the tissues from the deleterious effect of high polyamine levels and improve the activities of the cellular antioxidative system.
  Hamdan I. Al-Mohammed and Magdy M. Youssef
  A large number of mammals are hosts of taeniidae cestodes. Nematotaenia dispar was the only worm isolated from the infected gray monitor in Saudi Arabia. The present study was used to examine, the electrophoretic patterns of extracted proteins of Nematotaenia dispar isolates from Varanus griseus by using SDS-PAGE technique. The whole worm was washed with phosphate buffered saline (PBS, pH 7.4), weighed and homogenized by glass-glass homogenizer in cold physiological saline. Supernatants were examined by Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) and coomassie blue staining. The SDS-PAGE of the extracted proteins resulted in a total of eleven protein molecules were detected in gel, six bands with apparent molecular weights of 200, 116, 97, 66, 45 and 29 kDa were observed. It is concluded that the present results may give an idea about this worm that we can do antibodies for one of these proteins to be used in the future. In addition, further studies on the functions of these proteins are required.
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