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Articles by M.Z. Karim
Total Records ( 4 ) for M.Z. Karim
  M.Z. Karim , M.N. Amin , M.A. Hossain , S. Islam , Faruk Hossin and R. Alam
  Protocol for induction of callus and regeneration response of two sugarcane varieties (Isd-16, Isd-28) was established through callus culture using leaf sheath. Multiple shoot regeneration at various frequencies was observed using different concentrations and combinations of growth regulators. The highest percentage of callus induction was observed in the medium containing 3.0 mg l -1 2,4-D with 10% coconut water (CM). The best response in terms of multiple shoot formation was observed that on MS medium supplemented with BAP 1.0 mg l -1 +IBA 0.5 mg l -1. NAA (3.0 mg l -1) was found effective in the production of roots. The variety Isd-16 showed better response than the variety Isd-28 towards shoot multiplication. Seventy percent of the plantlets produced from in vitro culture method survived in the ex vitro condition.
  M.Z. Karim , M.N. Amin , M.A.K. Azad , F. Begum , M.M. Rahman , M.M. Islam and R. Alam
  Shoot multiplication of Chrysanthemum morifolium was achieved from the nodal and shoot tip explants of mature plant using MS medium with different concentrations and combinations of growth regulators. Maximum frequency of explants produced axillary shoot and the highest number of shoots per explant were obtained when MS medium fortified with 1.0 mg l-1 BAP.The combination BAP+GA3 was found effective result. But Kinetin (Kn) showed low performance for producing multiple shoots. The degree of shoot formation was affected by explant types and the exogenous hormonal regime in the medium.
  M.M. Rahman , M.N. Amin , M.A.K. Azad , F. Begum and M.Z. Karim
  Leaf segments from field grown mature plants of Elaeocarpus robustus Roxb. were used to initiate cultures for inducing callus and subsequent differentiation of shoot buds. Callus production was obtained within seven weeks and adventitious shoot formation was observed after five weeks of incubation on ½ MS medium supplemented with 0.5 mg l-1 BA + 0.1 mg l-1 NAA + 15% CW. Complete plantlets were obtained upon transfer of shoot cuttings on ½ MS with 0.1-2.0 mg l-1 IBA, NAA, IAA or without any auxin. The best and healthy rooting was observed on ½ MS medium supplemented 0.2 mg l-1 IBA. In vitro regenerated plantlets were transferred to potting soil and successfully established under natural condition with about 60% survival.
  R. Baksha , R. Alam , M.Z. Karim , Sk. A. Mannan , B.P. Podder and A.B.M.M. Rahman
  Microshoots were cultured on MS medium supplemented with different combinations of auxin, sucrose and pH level for in vitro rooting of callus induced sugarcane (Saccharum officinarum) variety namely Isd 31. Among the types and concentrations of auxin used the best rooting in all the microshoots was obtained with half strength (major salts) MS basal medium supplemented with NAA at 5.0 mg l‾1, sucrose at 50 g l‾1 and pH level 5.7 were the optimum conditions for rooting when microshoots were incubated 25+ 2°C under 16 h photo period regime. The regenerated plantlets were successfully transferred to soil and the percentage of survivability under ex vitro conditions was 75.
 
 
 
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