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Articles by M.Y. Ina-Salwany
Total Records ( 7 ) for M.Y. Ina-Salwany
  M.Y. Ina-Salwany , H. Hishammuddin , Z. Zulperi , M. Salema , M. Karim and F.M.I. Natrah
  In the present study, two strains of Malaysian Paenibacillus spp. isolated from intestinal tract of cultured hybrid red tilapia were tested for their antagonistic activity against Vibrio alginolyticus, a destructive bacterial pathogen to marine fishes as probiotics in aquaculture. Homology searches of 16S rRNA and Internal Transcribes Spacer (ITS) genes sequencing with nucleotide Basic Local Alignment Sequence Tool (nBLAST) revealed that both strains were identical to Paenibacillus spp. and Paenibacillus pabuli, respectively. In vitro and in vivo experiments were conducted to study the efficacy of these Paenibacillus strains as probiotics. Co-culture assay demonstrated that the growth of V. alginolyticus was inhibited when grown with these probionts. A complete inhibition of the pathogen by P. pabuli strain D12 occurred at 48 h of incubation period. To qualify strains as probiotics, Artemia challenged with V. alginolyticus was performed, with or without these potential probiotics. The highest survival rate (72%) of Artemia was observed in P. pabuli strain D12 treatment after challenged with V. alginolyticus, followed by P. pabuli strain D14 at 68% survival rate. The lowest survival of Artemia (23% survival rate) was recorded when challenged with only V. alginolyticus. This study reported the ability of Paenibacillus D12 and D14 as potential probiotics to control V. alginolyticus in Artemia culture system.
  V.H. Bernard , A. Nurhidayu , M.Y. Ina-Salwany and Yasser Abdelhadi
  The present study evaluated the inhibitory effect of Bacillus cereus strain JAQ04 against several pathogenic bacteria for tilapia under in vitro conditions. B. cereus strain JAQ04 was tested against Vibrio alginolyticus, Aeromonas hydrophila and Pseudomonas putida in a cross streaking assay, where B. cereus showed a strong antagonistic effect against the three tested bacterial fish pathogens. In the following test, B. cereus strain JAQ04 at 103, 105 and 107 CFU mL-1 was then tested against V. alginolyticus at 103 CFU mL-1 for 6 days in co-culture assay. The results revealed that higher concentrations (105 and 107 CFU mL-1) of B. cereus strain JAQ04 had higher inhibitory effect against pathogenic V. alginolyticus. Bacillus cereus strain JAQ04 was administered to juvenile tilapia to determine its safety for tilapia. The results showed that the Relative Percent Survival (RPS, %) was 70.83+18.2 which was not significantly different from the RPS of fish in the control group (78.50+3.5%).
  Lim Char Yee , M.Y. Ina-Salwany and Yasser M. Abdelhadi
  Four bacterial isolates, which proved to have the potential to be used as probiotics, were used to study their genotypic characterization using analysis of 16S rRNA gene sequence, antagonistic ability and safety when applied on tilapia via immersion. Gram staining showed that all 4 probionts were Gram positive bacteria. Three probionts were rod in shape and the fourth was cocci in shape and arranged in grape-like cluster. These 4 probionts were also identified using 16S rRNA gene sequencing and their identities were Paenibacillus barcinonensis strain D12, Paenibacillus sp. strain D14, Staphylococcus cohnii strain B11 and Bacillus megaterium strain E28, respectively. All of the probionts were examined for their antagonistic ability against pathogenic bacteria (Vibrio alginolyticus ATCC 33839, Aeromonas salmonicida and Aeromonas hydrophila ATCC 35654) under in vitro conditions by using cross-streaking method. P. barcinonensis strain D12 and Paenibacillus sp. strain D14 have shown stronger antagonistic ability than S. cohnii strain B11 and B. megaterium strain E28 in the antagonism test. P. barcinonensis strain D12 and Paenibacillus sp. strain D14 were chosen to test their safety on tilapia due to their better performance in antagonism test. Both probionts, P. barcinonensis strain D12 and Paenibacillus sp. strain D14, were safe for tilapia.
  R. Nehlah , M.Y. Ina-Salwany and Z. Zulperi
  Vibriosis is one of the most prevalent fish diseases caused by bacteria from the genus Vibrio. An effective method to counter Vibriosis is vaccination which increases the resistance of the immune system to subsequent pathogen infection. In this study, Vibrio alginolyticus strain VA2 isolated from infected tiger groupers was selected for determination of the antigenicity and molecular characterization of its Outer Membrane Proteins (OMPs). Protein profiling of OmpK and OmpW were evaluated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) while their antigenicity were tested by Western Blot. Results of the Western Blot assay revealed three antigenic OMP bands about 23, 31 and 34 kDa. Sequencing results showed that the full length of the target genes OmpK and OmpW of V. alginolyticus strain VA2 were 846 and 642 bp, respectively. Multiple alignment analysis showed that both regions of OmpK and OmpW were highly conserved among Vibrio species, thus are good candidates for vaccine development. Phylogenetic analysis of OmpK and OmpW showed that most branches in which they were grouped with had bootstrap values higher than 50%, indicating high confidence level of the branches. The antigenic sites of OmpK and OmpW proteins were predicted which were 34 and 27, respectively, thus are good candidates for vaccine development.
  N.M.R. Fazielawanie , S.S. Siraj , S.A. Harmin , M.Y. Ina-Salwany and N.S. Nik-Daud
  A study was conducted to develop and validate a competitive enzyme-linked immunosorbent assay (ELISA) for detection of plasmatic vtg in Asian sea bass, Lates calcarifer. Highly specific polyclonal antibodies against purified vtg (antigen) were employed for quantifying the concentration of plasma vtg. The working ranges of the assay were 31.2 to 1000 ng mL-1 with the sensitivity of 6.9 ng mL-1. Antigen concentration of 250 ng mL-1 and antibody dilution of 1:1000 were selected as a workable ELISA after several preliminary test. The ELISA demonstrated precision with intra-and inter-assay Coefficient of Variations (CVs) at 90, 80 and 50% of binding were less than 8.4 (n = 9) and 12.1% (n = 5), respectively. Serial plasma dilutions from natural vitellogenic females and E2 treated were paralleled to the vtg standard curve (purified vtg) as analyzed by ANCOVA (p<0.05). No cross-reaction was observed in analyses of male’s plasma, indicating non-specific binding. The assay was validated by measuring plasma vtg levels in matured females and males (n = 5) obtained during the reproductive season in captive condition. Female’s plasma vtg ranges from 0.9 to 1.54 mg mL-1, while no vtg was detected in males plasma. Our results indicated that vtg levels proposed as an indicator for maturing female Asian sea bass, L. calcarifer as well as in female species from genus Lates.
  N.A. Muhammad , A. Christianus , S.K. Daud , C.R. Saad , S.A. Harmin and M.Y. Ina-Salwany
  Vitellogenin (vtg) is a high molecular weight glycophospholipoprotein synthesized in the liver under stimulation of estrogen. Basically found in sexually mature female, vtg being taken up by developing oocyte during maturation. It functioned as a nutrient storage for growing embryo. Having potential to be used as a maturation indicator, vtg helps to enhance fish fry production. Tor tambroides is one of the most sought after fish in Malaysia for having potential as a game fish yet so important economically due to high demand by farmers as well as consumers. Main problem in the mass production of T. tambroides fry in hatchery is in the availability and selection of matured broodstock. Identification of matured and ready females morphologically can only be done by experience workers. Therefore, establishment of simple technique to definite identify matured females is necessary. This has led to the identification, purification and development of enzyme linked immunosorbent assay (ELISA) to measure blood plasma vtg as maturation indicator. This will definitely contribute to the hatchery production of T. tambroides fry.
  Nurul Shazwani , Mohamad Pipudin , M.Y. Jasmin , M.Y. Ina-Salwany , S.A. Harmin and Murni Karim
  Vibriosis caused by Vibrio alginolyticus has become one of the most threatening diseases that could limit the production of marine fish in aquaculture industry. In this study, microbe strains Micrococcus spp. (JAQ07) and Bacillus spp. (JAQ04) were used as potential probiotics. Both potential probionts were identified as gram-positive bacteria with different morphology. The antagonistic ability of each candidate probiotics towards V. alginolyticus (ATCC33839) were conducted in liquids modes via co-culture assay in three different concentrations of probiont (102, 104 and 106 CFU mL–1) and each concentration was inoculated with 105 CFU mL–1 of V. alginolyticus. The effectiveness of antagonistic activity was measured by the reduction of V. alginolyticus colonies via plate count at 24 h interval for 120 h. The co-culture assays revealed the reduction of V. alginolyticus colonies by both probiont strains compared to the control (V. alginolyticus at 105 CFU mL–1). In in-vivo assay, JAQ04 was able to enhance the survival of Artemia compared to JAQ07 after challenged with V. alginolyticus. Results revealed that at seven days after inoculation, Artemia treated with at 106 CFU mL–1 cell density followed by challenged with V. alginolyticus showed 70% of survival, while Artemia challenged with only V. alginolyticus demonstrated a 20% survival rate. Since, both strains displayed excellent probiotic activities, they are indeed suitable probiont candidates for managing Vibriosis infecting marine fish.
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