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Articles by M.X. Chen
Total Records ( 5 ) for M.X. Chen
  M.X. Chen , J.X. Chen , Y.G. Ye , S.T. Gao , Y.J. Geng , D.N. Huang , X.H. Li , R.L. Zhang , X.Q. Zhu and M.J. Xu
  The aim of the present investigation was to examine the prevalence of anisakid larvae in marine fishes sold in Shenzhen in March to November 2008. A total of 394 individual fishes representing 40 fish species were obtained from different wholesale markets. They were examined for the presence of anisakid larvae by examination of the viscera and whole body cavity. About 23 species of fish (57.5%, 23/40) namely Decapterus maruadsi, Harpodon nehereus, Muraenesox cinereus, Trichiurus haumela, Pneumatophorus japonicus, Sebastods schlegeli and so on were found to be infected with anisakid larvae with an overall infection rate of 31.0% (122/394). The results of present investigation demonstrated the risk of human infection with anisakid larvae in Shenzhen, China which have implications for implementing control strategies against anisakid infection in humans in China.
  L. Ai , S.H. Chen , Y.N. Zhang , X.N. Zhou , H. Li , M.X. Chen , J. Guo , Y.C. Cai , X.Q. Zhu and J.X. Chen
  The present study examined sequence variations in the Internal Transcribed Spacers (ITS) of nuclear ribosomal DNA (rDNA) and two mitochondrial DNA (mtDNA) regions, namely cytochromec oxidase subunit 1 (cox1), NADH dehydrogenase subunits 1 (nad1), among Metorchis orientalis metacercaria isolates from Guangxi in China. The sequences of ITS, pcox1 and pnad1 were amplified from 6 individual M. orientalis metacercariae and sequenced. The relevant sequences of other 7 trematode species belonging to 6 genera in 4 families were downloaded from GenBank and their phylogenetic relationships were re-constructed using the combined pcox1 and pnad1 mt DNA sequences with Trichinella spiralis as outgroup. The results showed that sequences of ITS rDNA, pcox1 and pnad1 of M. orientalis were 1131, 654 and 650 bp, respectively and they were quite conserved among the M. orientalis isolates. However, they were quite different from that of other species, phylogenetic analysis of the combined pcox1 and pnad1 mt DNA sequences were able to distinguish M. orientalis from different species of the Opisthorchiidae and trematodes in other families. Therefore, the ITS, cox1 and nad1 mt DNA sequences provide effective genetic markers for the specific identification of trematodes of the Opisthorchiidae family and have implications for studying their population biology, genetic structure, as well as molecular epidemiology.
  C.Y. Liu , R.L. Zhang , M.X. Chen , J. Li , L. Ai , C.Y. Wu , X.Q. Zhu and R.Q. Lin
  The present study examined sequence variations in the Internal Transcribed Spacers (ITS) of nuclear ribosomal DNA (rDNA) among Angiostrongylus cantonensis isolates from Shenzhen, Qingyuan, Jiangmen and Wenzhou in China. The ITS of nuclear rDNA was amplified from individual A. cantonensis by Polymerase Chain Reaction (PCR) and the representative amplicons were cloned and sequenced. The length of the ITS sequences was 1593-1614 bp for all Chinese A. cantonensis specimens and these sequences were composed of complete ITS-1 sequence of 712-720 bp, complete 5.8 S sequence of 153 bp, complete ITS-2 sequence of 633-650 bp and partial 28 S sequence of 70 bp. The intra-specifc sequence variation in A. cantonensis was 0.1-1.0% for ITS-1 and 0.0-1.3% for ITS-2 whereas sequence comparison revealed that the inter-specifc sequence differences were higher: 15.0-34.6% for ITS-1 and 22.7-24.2% for ITS-2 between A. cantonensis and other Angiostrongylus sp. The results showed that the ITS sequences were conserved among the A. cantonensis isolates however, they were quite different from that of other Angiostrongylus species. Therefore, ITS sequences could provide useful genetic markers for the specific identification and genetic characterization of Angiostrongylus sp.
  Y.Z. Cheng , J. Hou , X.H. He , Z.K. Hong , L.S. Li , G.H. Lin , M.X. Chen and S.H. Chen
  Paragonimiasis and angiostrongyliasis are important food-borne parasitic diseases in a number of countries including China. Both diseases are transmitted by freshwater and terrestrial intermediate snail hosts. In the present investigation, we examined the prevalence of Paragonimus and Angiostrongylus cantonensis in the intermediate host snails in southeastern China’s Fujian province where paragonimiasis and angiostrongyliasis are endemic. The prevalence of P. westermani cercariae in the stream-type snail Semisulcospira libertine found in the breeding grounds in Shouning county was 0.09% in the upstream and 0.56% in the downstream, respectively. For pit-ditch type, the prevalence of P. westermani cercariae was 0.19% and 0.82% in upstream and downstream, respectively. The snail Tricula xiaoqiaoensis was infected with Paragonimus skrjabini cercariae with prevalence of 0.33% in the pit-ditch type and 1.52% in the seepage type in Zhenghe county. Prevalence of A. cantonensis larvae in Achatina fulica in Nan’an county in garbage stack, vegetable plot and crop land were 83.54, 39.53 and 19.23%, respectively. In Lianjiang county, prevalence of A. cantonensis larvae in snails Pila polita in trench, paddy field, residential ditch, vegetable irrigation ditch and pond in the same river system were 4.08, 8.82, 75.34, 34.04 and 5.56%, respectively. The snail Bellamya lithophaga found in the western suburb of Fuzhou city was identified as the new intermediated host for A. cantonensis with a prevalence of 14.38%. Demonstration of prevalence of Paragonimus and A. cantonensis in wild snails in Fujian province poses substantial risk for future outbreaks of the two food-borne parasitic diseases.
  L. Ai , M.X. Chen , S.H. Chen , Y.N. Zhang , H. Li , Y.C. Cai , Y. Lu , L.G. Tian , X.N. Zhou and J.X. Chen
  MicroRNAs (miRNAs) are small non-coding RNA which generate from large hairpin precursors and play important role on post-transcriptional regulators of target genes. Although, many individual miRNAs have recently been extensively studied, there has been very little research on miRNA transcriptomes in trematode. By the method of using high throughput Solexa sequencing technology, 11.02 million clean reads have been obtained of Paragonimus westermani. Among the clean reads, 1.14 million ones (10.37%) were perfectly mapped onto the S. japonicum genome which included 20,322 (0.69%) unique sequences. A number of 9,492,613 (86.17%) reads had no match with public databases and marked as un-annotated RNAs. Nucleotide bias analysis found that the known miRNAs showed high bias and the guanine was the dominant nucleotide, particularly at the 2nd and 23rd positions which were almost at the beginning and end of conserved miRNAs. Three novel miRNA corresponding to 18 precursors were predicted. To the knowledge, this is the first report of miRNA profiles in P. westermani which will contribute to better understanding of the complex biology of this zoonotic parasite. The reported data of P. westermani miRNAs should provide valuable references for miRNA studies of closed related zoonotic trematode.
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