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Articles by M.T. Hossain
Total Records ( 4 ) for M.T. Hossain
  M.A. Islam , M.A. Samad , M.B. Rahman , M.T. Hossain and S. Akter
  The duck plague vaccine (DPV) is locally produced from the Livestock Research Institute (LRI), Mohakhali, Dhaka which is used to control the duck plague viral disease in ducks in Bangladesh. Efficacy of this vaccine reports has no, so to say on ducks in Bangladesh. Three weeks old 50 Khaki Cambell Ducks were used to evaluate the induced of immune responses of duck plague vaccine during the period from February to April 2003. These 50 ducks were divided into three groups (A = 15, B = 15 and C = 5 ducks) and each duck of group A and group B were inoculated primarily with duck plague vaccine @ 1.0 ml. intramuscularly at the age of 4 weeks and whereas ducks of group C served as unvaccinated control. Then each duck of group A was also injected booster dose after 2 weeks of primary vaccination with same vaccine, dose and route. The mean value of TLC, TSP and PHA antibody titre of ducks of group A was found significantly (p < 0.1) increased at two weeks and four weeks of post-primary vaccination and two weeks and four weeks of post-booster vaccination in comparison to the pre-vaccination values. The mean value of TLC, TSP and PHA titre of ducks of group B was also found significantly increased (p < 0.1) at two, four, six and eight weeks of post-primary vaccination in comparison to the pre-vaccination values. These results indicate that booster vaccination of duck plague vaccine induced comparatively higher TLC, TSP and PHA antibody titre than single primary vaccination in ducks. The mean value of TLC, TSP and PHA in unvaccinated control group C was more or less nearer at the age of four weeks and 12 weeks in ducks. These results showed that the locally prepared duck plague vaccine induced sufficient both cellular and humoral immune responses with booster-vaccination than primary vaccination in ducks. Therefore, it could be recommended to booster dose of vaccination of duck plague vaccine to control duck plague under field condition in Bangladesh.
  M.T. Hossain , M.A. Islam , M.M. Amin and M.A. Islam
  The comparative efficacy between the conventional vaccine (DLS-DPV) and experimentally prepared duck plague vaccine (BAU-DPV) was evaluated in seventy-five 35-day-old Zinding breed ducklings during the period from October/02 to March/03. The ducklings were equally divided into five groups (A, B, C, D and E). Ducklings of group A and B were primarily vaccinated with 0.5 ml and 1.0 ml of DLS-DPV respectively and those of group C and D were primarily vaccinated with 0.5 ml and 1.0 ml of BAU-DPV. The ducklings of group E were kept as unvaccinated control. Five months after primary vaccination all the ducks of vaccinated groups were boosted with 1.0 ml of DLS-DPV and BAU-DPV and 21 days after booster vaccination all the ducks of vaccinated and control groups were challenged with 1ml of 10 4 EID50 of virulent field isolate of duck plague virus (DPV). The level of immunity developed in different vaccinated groups of ducks was measured by passive hemagglutination (PHA) test. The mean PHA titre of birds of group A, B, C and D after primary vaccination were 38.4 ± 6.4, 28.8 ± 3.2, 51.2 ± 7.84 and 38.4 ± 6.4 and after booster vaccination were 153.6 ± 25.6, 76.8 ± 12.8, 358.4 ± 62.71 and 115.2 ± 12.8 respectively. Results of PHA test indicated that experimentally prepared duck plague (BAU-DPV) vaccine revealed higher immune response compared to that of the conventional (DLS-DPV) vaccine and results of the challenge experiment indicated that the mean PHA titre over 100 after booster vaccination revealed 100% protection.
  G.M. Nooruddin , M.T. Hossain , M. Mohammad and M.M. Rahman
  Sero-epidemiological investigation was carried out to determine avian influenza virus in native chickens at Dagonbhuyian, Feni district in Bangladesh. A total of 224 sera samples were collected from the chickens of key beneficiaries during monsoon, winter and summer seasons. All sera samples were examined for the detection of antibodies of Avian influenza virus by indirect enzyme linked Immunosorbant assay using commercially available Kits. The seroprevalence was found 25% in Ram Nagar union, 14.28% Matubhuyian union, 0% in Rajapur union and 0% Jaylashker union. The overall seroprevalence of avian influenza was recorded 9.82%. Sero-epidemiology of virus in relation to seasonal variation was also carried out in the study areas and it was recorded 14.45% during monsoon, 3.70% during winter and 11.67% during summer season. The investigation exhibited higher prevalence of avian influenza in hens (10.83%) than cocks (8.65%). The investigation revealed the highest (12.80%) prevalence in birds > 34 weeks of age group and the lowest (3.13%) in birds of 8-16 weeks of age. However, Quick antigen detection kit for avian influenza virus identification did not show positive results of the samples collected for virological study.
  M.A. Hossain , M.T. Hossain and N. Absar
  The lectin, MSL-1 was subjected to various chemical modifications in order to ascertain the amino acid residues responsible for their hemagglutinating activity. Modification of MSL-1 with acetic anhydride blocked nineteen amino groups and five tyrosine residues per molecule of lectin and decreased complete hemagglutinating activity. De-O-acetylation regenerated four of the tyrosine residues and resulted in a recovery of 80% activity. The presence of inhibitory saccharide galactose, a significant protecting effect was observed and only 1.49 tyrosine residues and fifteen amino groups were found to be modified with significant retention of hemagglutinating activity. The treatment of lectin with citraconic anhydride showed that nineteen amino were modified with the loss of 25-30% hemagglutinating activity. Modification of lectin with N-acetyl imidazole resulted in acetylation of fifteen amino groups and six tyrosine residues per molecule. De-O-actylation also regenerated 4.5 tyrosine residues with the retention of 75% of its hemagglutinating activity. When modification was conducted in the presence of galactose, about 3.5 tyrosine residues were protected from modification with 80% hemagglutinating activity. Successive addition of NBS to MSL-1 solution resulted in the modification of five tryptophan residues per molecule of lectin at pH 6, 5 and 4, respectively with the loss of 20-30% hemagglutinating activity. With DEPC at pH 7.2, eight histidine residues were modified and in the presence of inhibitory saccharide galactose, five histidine residues were protected from modification with the retention of 90% hemagglutinating activity. This was further confirmed from the finding that the activity was regenerated when His-modified MSL-1 was treated with hydroxylamine. The overall modification studies indicated that four tyrosine and five histidine residues were located at the saccharide-binding site of the lectin. However, modification of tryptophan and lysine had no effect on the hemagglutinating activity.
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