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Articles by M.S.R. Khan
Total Records ( 2 ) for M.S.R. Khan
  M.K. Hossain , M.M. Amin , M.S.R. Khan , M.R.R. Sarker , Y.Akhter and S.M.H. Rashid
  The aim of the study is to detect the causes of vaccination failure in chickens against IBD in Bangladesh. One selected local field IBDV isolate, one commercially available live attenuated IBDV vaccine Bur-706 (RP) and one killed IBDV vaccine Gumboriffa (RP) were used in this study. A total of 100 chicks (Star brow) of 0,7,14,21 and 28 days age group (taking 20 in each group) were used for study the serologic response with a selected local field IBDV isolate. Similarly two other vaccines were vaccinated in each 100 chicks of similar age groups. After collection of sera weekly for upto six weeks from all groups of birds they were subjected to SNT, PHA test and AGPT against a specific IBDV antigen. Through this investigation it is found that the local field IBDV isolate demonstrate higher serologic response than that of commercially available live or killed IBDV vaccines. So, it may be concluded that the commercially available imported vaccines can not induce sufficient immune response to protect the chickens in Bangladesh. It may be due to degradation of the vaccine quality during transportation or in a new environmental condition or due to antigenic dissimilarities among the local field virus and the imported vaccines.
  M.F.R. Khan , M.B. Rahman , M.S.R. Khan , K.H.M.N.H. Nazir and M. Rahman
  Antibiogram study and plasmid profile analyses were conducted to find out the correlation of the recently isolated Salmonella organisms of Bangladesh. Antibiogram study revealed that the isolates were highly sensitive to Ciprofloxacin, Cephalexin and Kanamycin. In the plasmid profile analysis it was observed that out of 4 isolates of Mymensingh district (M1 to M4), 3 isolates showed 3 plasmid bands in each and 1 isolate showed 4 plasmid bands. The estimated size of the plasmid bands of M1, M2, M3 and M4 were (33.5, 24.50, 4.0, 3.0); (33.5, 3.0, 1.0); (33.5, 3.0, 1.0) and (5.0, 4.0, 1.8) kb, respectively. On the other hand among the isolates of Feni district (F1 to F9), 6 isolates (F4, F5, F6, F7, F8 and F9) showed 5 number of plasmid bands in each and the estimated plasmid band size were same (33.5, 5.0, 4.0, 2.0, 1.8). The plasmid bands of other 3 isolates (F1, F2 and F 3) were found to be different and the estimated size were recorded as 4 (33.5, 5.0, 1.6, 1.5); 3 (33.5, 1.6, 1.5) and 1 (0.7), respectively. Among the isolates of Dhaka district (D1 to D11); no plasmid band was found to be detected in 3 isolates (D2, D5 and D6). The number of plasmid bands of other isolates of D1, D3, D4, D7, D8, D9, D10 and D11 were 4 and the estimated size were determined as (33.5, 15.0, 2.0, 1.0); 5 (33.5, 15.0, 3.0, 2.0, 1.0); 5 (33.5, 5.0, 4.0, 1.8, 1.3); 3 (33.5, 3.0, 1.0); 3 (33.5, 3.0, 1.0); 1(33.5); 2 (33.5, 3.0) and 2 (33.5, 4.0) kb, respectively. All these isolates were tested for antibiotic sensitivity against 8 commonly used antibiotics belonging to different groups. From the antibiogram study it was revealed that all the isolates of Mymensingh and Dhaka district were 100% resistant to Cloxacillin and among the isolates of Feni district 100% were resistant to Erythromycin and Cloxacillin. Plasmid profile analysis of the isolated Salmonella organisms revealed that the isolates carrying multiple plasmids which might be the cause of various degree of antibiotic resistance.
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