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Articles by M.S. Norfitriah
Total Records ( 2 ) for M.S. Norfitriah
  M.S. Norfitriah , M. Hair-Bejo and A. Majdi
  Highly pathogenic Fowl Adenovirus (FAdV) is a causative agent of Inclusion Body Hepatitis (IBH) in poultry. It causes mortality and poor performance of the affected chickens. It was objectives of the study to determine pathogenicity of FAdV serotype 8b isolates of Malaysia in Specific Pathogen Free (SPF) chickens. The virus isolates namely, UPM11134 and UPM11142 were obtained from IBH outbreaks in broiler chicken farms and characterized as FAdV serotype 8b. The liver samples of affected chickens were prepared and inoculated into SPF embryonated chicken eggs via. Chorioallantoic Membrane (CAM) route. The liver of embryos was subsequently harvested for preparation of virus inoculum. The 36 days old SPF chicks were divided into 3 groups namely groups A-C. Each group was further divided into sacrificed and mortality groups. Chickens in groups A and B were inoculated with UPM11134 and UPM11142 FAdV isolates, respectively, via. intraperitoneal route at day old. All chicken in group C remained uninoculated and acted as the control group. The chicken were monitored for any clinical abnormalities throughout the trial. Gross lesions were recorded on necropsy and samples of liver and gizzard were collected and fixed in 10% buffered formalin for histological examination. The study showed 100 and 91% mortality of SPF chickens in groups A and B, respectively, at day 4 post inoculation (pi). Clinical signs of IBH such as depression, weakness, ruffled feathers and diarrhea were recorded within 12-24 h prior to death. On necropsy, hydropericadium with pale, friable and petechial haemorrhages of liver were recorded. Hepatitis with areas of hepatic necrosis and haemorrhages and presence of numerous basophilic Intranuclear Inclusion Bodies (INIB) in degenerated hepatocytes were recorded in all chickens in groups A and B, whilst INIB were also observed in glandular epithelium of gizzard in group A. All chicks in group C remained normal throughout the trial. It was concluded that Malaysian FAdV serotype 8b isolates are highly pathogenic in SPF chickens and acted as the primary agent of IBH.
  M.S. Norfitriah , M. Hair-Bejo and C.Z. Zhe
  Fowl Adenovirus (FAdV) is a non-enveloped DNA virus which is the primary pathogen of Inclusion Body Hepatitis (IBH) in chickens. IBH outbreaks were reported worldwide and was first reported in Malaysia in 2005 due to FAdV strain of serotype 8b infection. It was objective of the study to determine pathogenicity and immunogenicity of live attenuated and/or inactivated FAdV strain of serotype 8b (UPM1137) of Malaysian isolate in commercial broiler chickens. The 54, 1-day-old Cobb 500 broiler chicks were divided into four groups, namely groups A-D. Feed and water were provided ad-libitum. The chicks in groups A-C were inoculated with inactivated FAdV (0.2 mL) with virus titer of 106.5 TCID50 /0.2 mL, live attenuated FAdV (0.1 mL) with virus titer of 105.2 TCID50 /0.1 mL and the combination of the inactivated (0.2 mL) and live attenuated (0.1 mL) FAdV, respectively at day old and day 14 post-inoculation (pi). Body weight and blood samples were collected prior to necropsy at days 14 and 28 pi, except sampling was also conducted at day 0 pi in the group D (control). On necropsy, the gross lesions and liver weight were recorded and samples of liver were collected for histological examination. The study showed that neither clinical signs nor gross and histological lesions were recorded in all group of chickens throughout the trial. The body weight of chickens at days 14 and 28 pi were not significantly different (p>0.05) among all the groups. The liver to body weight ratio of group C was significantly higher (p<0.05) than groups A and D at day 28 pi. The FAdV antibody titer in group D (control) was 938±1596 on day old and was not detected at days 14 and 28 pi. However, the FAdV antibody was induced at high titer in all the inoculated groups at days 14 and 28 pi. The FAdV antibody titer of group C was significantly (p<0.05) higher than groups A and B at day 28 pi. It was concluded that the live attenuated and inactivated FAdV are safe and able to induce FAdV antibody titer in broiler chickens with moderate level of maternally derived antibody at day old of age. The combination of live attenuated and inactivated FAdV was able to induce higher antibody titer when compared to sole use of live attenuated or inactivated FAdV. It has high potential to be used as vaccination strategy against IBH outbreaks.
 
 
 
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