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Articles by M.R. Karim
Total Records ( 4 ) for M.R. Karim
  M.F.M. Zain , M.R. Karim , M.N. Islam , M.M. Hossain , M. Jamil and H.M.A Al-Mattarneh
  This study describes the development of statistical models to predict strength and slump of silica fume incorporated High-Performance Concrete (HPC). Experimental data of silica fume incorporated HPC mixes were used to develop and validate models. The HPC having compressive strength range of 40-113 MPa and slump range of 180-250 mm were used. Statistical models were developed by regression analysis. The results of prediction by the models showed good agreement with those of experiments and other researchers. The developed models can be used to predict slump and 28 days compressive strength of silica fume incorporated HPC.
  M.R. Karim , M.A. Islam , N. Absar and F. Hashinaga
  A protease from tomato flesh was purified to homogeneity by (NH4)2SO4 precipitation followed by Sephadex G-75 and DEAE-cellulose column chromatography. Molecular weight of the enzyme was estimated to be 81-79 KD by gel filtration and SDS-PAGE respectively. The enzyme was found to be a single polypeptide chain as revealed by SDS-PAGE under either reducing or non-reducing conditions. Optimum activity was observed at pH 7.0 and 45°C with a km value of 0.48 per cent determined by using casein as substrate. The enzyme appears to be a serine protease being inhibited greatly by DFP and PMSF and to a lesser extent by heavy metals such as Pb2+ and Fe2+.
  M.A. Razzaque , Z.A. Saud , N. Absar , M.R. Karim and F. Hashinaga
  Polyphenoloxidase (PPO), an important oxidative enzyme which played a vital role in the brewing of guava after infection with fruit-rot disease was purified from disease-affected guava. The method was accomplished by ion exchange chromatography of crude enzyme extract on DEAE-cellulose followed by CM-cellulose. The purified enzyme was found to be homogeneous as judged by polyacrylamide disc gel electrophoresis. Molecular weight of the enzyme was estimated to be 178 kDa and 180.5 kDa by gel filtration and SDS-PAGE respectively. The enzyme appeared to be a single polypeptide chain as revealed by SDS-PAGE either in presence or in absence of reducing agent. Optimum activity of the enzyme was at 48°C and pH around 8. Activity was destroyed completely in presence of EDTA, a metal chellator. Purified PPO showed an estimated km value of 4.4 mM for catechol as substrate.
  M.R. Karim , S.M. Asaduzzaman , F. Islam , M.A. Islam , S.M.N. Islam and Q. Naher
  Two types of fungicides and their mixture viz. Rovral, Ridomil and Rovral+Ridomil along with five combinations of different fertilizers were tested to find the best integrated package method for successful garlic production. The highest fertilizer dose especially nitrogenous fertilizer F5 (200, 166, 200, 20 and 1000 kg N, P2O5, K2O, S and Cow dung ha–1) produced the highest bulb yield of garlic (6.36 and 7.89 t ha–1 during 1997 and 1998 respectively), which was statistically similar with the yield of F4 (6.28 and 7.57 t ha–1 during 1997 and 1998 respectively) where 150, 166, 200, 20 and 1000 kg N, P2O5, K2O, S and CD ha–1 were applied. The mixed application of Rovral and Ridomil was better in case of garlic bulb yield (6.44 and 7.79 t ha–1 in 1997 and 1998 respectively). The incidence of purple blotch was significantly reduced (1.6 and 1.7 score in 1997 and 1998 respectively) by mixed application fungicide. The interaction between fertilizer and fungicide had no significant effect on garlic yield.
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