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Articles by M.R. Govindappa
Total Records ( 2 ) for M.R. Govindappa
  M.R. Govindappa , I. Shankergoud , K.S. Shankarappa , W.A.R.T. Wickramaarachchi , B. Anjeneya Reddy and K.T. Rangaswamy
  The present study was conducted to investigate the etiology, mode of transmission and molecular relationship of the causal virus associated with Sunflower leaf curl disease with other related viruses. Leaf curl disease on sunflower caused by a begomovirus was observed to an extent of 40% for the first time on some hybrids grown in experimental plots at Main Research Station (MRS), Raichur, Karnataka, Southern India. Symptoms like curling, malformation of leaves, leaf thickening, leaf enations and severe stunting were observed on infected plants. The disease was successfully transmitted by whitefly, Bemisia tabaci. The experimentally inoculated plants produced symptoms similar to that of naturally infected plants. The causal virus was detected using two sets of begomovirus specific degenerate primers. The core Coat Protein (CP) gene fragment of 575 bases was amplified from naturally as well as whitefly inoculated plants. Phylogenetic analysis of the core CP gene sequence of the virus with those of other begomoviruses clustered next to Tomato Leaf Curl Karnataka Virus isolate Lucknow (ToLCKV-(Luc) (Accession No. EU604297.2) and Tomato Leaf Curl Virus-Bangalore II (ToLCBV-(Ban2) (Accession No. EU604297.2) and shared 97.5% nucleotide identities. Thus, its exact taxonomic status will require sequencing of the complete ssDNA viral genome. The begomovirus infecting sunflower, which we tentatively called Sunflower Leaf Curl Virus (SuLCV).
  L.S. Vanitha , K.S. Shankarappa , K.T. Rangaswamy , W.A.R.T. Wickramaarachchi and M.R. Govindappa
  Sunflower leaf curl disease caused by begomovirus is one of the newly emerging viral diseases in Nothern Karnataka, Southern India. The disease has attracted lot of attention of the pathologists, as it affects the productivity of sunflower an important oilseed crop in the country. The present study was conducted to characterize the begomovirus causing sunflower leaf curl disease by cloning and sequencing the major genomic components. The Complete DNA-A and betasatellite components of begomovirus isolated from sunflower leaf tissue infected with leaf curl virus collected from Main Agricultural Research Station, Raichur, Karnataka, India was cloned and sequenced. The virus contained the DNA-A and the associated satellite beta DNA components of 2761 and 1373 nucleotides (nt) in length, respectively. The DNA-A molecule shared maximum identity with tomato leaf curl karnataka virus clone IKH12 (ToLCKV-[IKH12]) (HM803118) (97.13%) and clone IKB3 (ToLCKV-[IKB3]) (HM851186) (96.95%) from India. The betasatellite shared a high nucleotide identity (93.6- 94.07%) with Potato apical leaf curl betasatellite (PoLCB-[IN-CHI: 05]) and Papaya leaf curl betasatellite (PaLCuB-[IN-CHI:05]) associated with tomato leaf curl disease from India. The results indicate for the first time that a strain of ToLCKV together with beta DNA satellite causing sunflower leaf curl disease (SuLCD) in India.
 
 
 
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