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Articles by M.N. Haque
Total Records ( 5 ) for M.N. Haque
  M.N. Haque , U.K. Rima , M.Z. Hossain , M.S. Islam , S.M.Z.H. Chowdhury , M.M. Hossain and M.A.H.N.A. Khan
  Objectives: Avian tuberculosis (ATB) is caused by Mycobacterium a. avium, potentially zoonotic and requires adapting molecular techniques to detect pathogenic serovars and prevent zoonosis. Methodology: Layer chicken (N = 2000) of organized poultry farms of Mymensingh district showed clinical signs of progressive emaciation and reduced weight gain constituted the study materials. This study used necropsy, histopathology and Ziehl Neelsen staining to identify specific pathology of ATB in chicken. This study adapted a Polymerase Chain Reaction (PCR) technique to detect ATB in layer chicken due to highly pathogenic variant (serovars 1, 2 and 3) of Mycobacterium a. avium. Results: Investigation of sick birds at necropsy showed granulomas in liver, spleen and intestine and suspected as a case of ATB. Using histopathology, multi-focal accumulation of macrophages, epitheloid cell and lymphocytes were seen in liver, spleen, kidney, heart and intestine. Acid fast bacterium was detected in tissue sections of spleen, liver and intestine using Ziehl Neelsen staining but unable to differentiate infectivity due to pathogenic, low pathogenic and saprophytic variants of Mycobacterium. Visceral organs were, therefore, collected for PCR detection of specific cause of ATB. A specific PCR protocol was adapted targetting 16S rRNA gene (192 bp) and successfully detected pathogenic variant of ATB (M. a. avium) in clinically infected and carrier chickens. Conclusion: The PCR technique showed the potentiality to diagnosis pathogenic variant of ATB in a few hours with high degree of sensitivity and specificity. Pathogenic variant (serovars 1, 2 and 3) of ATB is highly contagious and potentially zoonotic. The PCR technique can be used to screen elderly layer chickens, diagnose ATV at early onset and dispose the infected flock to prevent future zoonosis.
  M.N. Haque , M.R. Gofur , K.M. Asaduzzaman and M.M.U. Bhuiyan
  The study was designed to determine the effects of breed, age, parity, feeding practice, Body Condition Score (BCS), timing of insemination, postpartum insemination interval and milk yield on first service pregnancy rates in cows (n = 308). The demographic factors were recorded by interviewing the farmers. All cows were examined for pregnancy diagnosis by rectal palpation of genital tract at 60-80 days post Artificial Insemination (AI). The pregnancy rate of different groups was compared and analyzed by Z test using SPSS software version 17. The overall pregnancy rate in cows was 52.6%. Though the age and parity of cows and milk yield did not affect significantly on pregnancy rate, the pregnancy rate was the highest in cows of 3-5 years of age (56.1%), in cows of parity 1-2 (57.4%) and in moderate yielding (2-5 liters milk/day) cows (62.1%). Balanced feeding and timing of insemination had a profound impact on the pregnancy rate of cows. The pregnancy rate in cows fed with combination of green grass, straw and concentrate was significantly (p<0.05) higher (63.5%) than cows fed only straw (38.5%). The pregnancy rate in cows with BCS 3-4 was significantly (p<0.05) higher (58.0%) than that of BCS 1.5-2 cows (35.0%). The pregnancy rate in cows inseminated at 6-12 h after the start of standing estrus was significantly (p<0.05) higher (58.8%) than those inseminated 13-24 h after start of standing estrus (40.4%). Cows with good BCS and AI at 6-12 h after the start of standing estrus are the best choice of selection for getting the best result in the first service pregnancy rate in cows.
  M.N. Haque , S.A. Aziz , S.S. Chanda , M.I. Hossain and M.A. Baset
  Various milking and reproductive performances were analyzed. The effects of concentrate feed were found significant on age at weaning AW (p<0.001) age at first heat (AFH) (P<0.05) age at first conception (AFH) (P<0.001) No. of services per conception (P<0.05), age at first calving (P<0.001) post partum heat period (PPHP) (P<0.001), calving interval (Cl) (P<0.001), daily milk yield (p<0.05) and lactation length LL (P<0.001). The effects of management level were significant on AW (P<0.001), PPHP (P<0.01), CI (P<0.001) and daily milk yield (P<0.05). The overall mean values were 245.80± 3.80 days for AW, 35.30±0.40 months for AFH, 37.20± 0.70 monthly for conception, 46.20±0.40 monthly for AF calving, 1.30±0.10 for no. of services for conception, 182.90± 4.80 days for PPHP, 15.50±0.20 months for CI, 2.50±0.30 kg for daily milk yield and 250.60±4.50 days for lactation length.
  M.A. Baset , M.M. Rahman , M.L. Ali , A.S.M. Mahbub and M.N. Haque
  Three groups of steers mentioned as T1, T2 and T3 were maintained with untreated straw (control), urea molasses straw (UMS) and UMS + wheat bran respectively. The results revealed that feed intake were more in case of T2 and T3 than in T1. Average live weight gain/animal/day were 204.17, 400.0 and 418.75g in case of T1, T2 and T3 treatment groups respectively. Live weight changes in T2 and T3 treatment groups differed significantly from 2nd month to the end of experiment at 1% level. Maximum live weight gain was observed at T3 treatment group where extra wheat bran was added. Daily live weight gain and selling price were more in the steers of T3 group but net return was observed maximum in the steers of T2 group. It may be concluded that combination of UMS and wheat bran had an influencing effect on live weight gain.
  M.S. Hossain , M.N. Haque , S.A. Aziz , M.S. Mazumder , M.L. Ali and A.T.M. M-E-Elahi
  Thirty-six indigenous postpartum cows were given dry straw ad libitum + 1.5 kg concentrates (control) or UMS ad libitum + 1.5 kg concentrates (treated). Cows were stall fed. Calves were tied up at night and allowed to free access or suckling to the cows during the whole day. The cows were milked only in the morning. Live weight change of cows and calves were recorded monthly and milk yield was recorded daily. The reproductive performance of cows was also studied. Live weight change of cows -57.40 and 37.75 g/d (P < 0.05); milk yield of cows 1.83 and 2.52 kg/d (P < 0.01); calf weight gain 96.99 and 139.35 g/d (P < 0.01); calving to first service interval 182 and 113 days (P < 0.01) ; calving to conception interval, 210 and 134 days (P < 0.01) and number of services per conception 2.11 and 1.94 (P >0.05), for controlled and treated groups respectively. It is concluded that UMS can be fed for improving the productive and reproductive performance of indigenous cows under the village conditions of Bangladesh.
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