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Articles by M.N. Anwar
Total Records ( 12 ) for M.N. Anwar
  W. Shumi , Md. Towhid Hossain and M.N. Anwar
  Following the enrichment technique in total 25 microbial colonies were isolated, purified, preserved and tested for their proteolytic ability. Among the bacterial isolate D1 showed the maximum ability to degrade egg albumin, milk casein and gelatin. On the basis of cultural, morphological, physiological and biochemical characteristics the isolate D1 was identified as Bacillus fastidiosus den Dooren de Jong. The isolate (D1) produced maximum protease at 37 ° C temperature and pH 7.0. Cellobiose (carbon source) and (NH4)2HPO4 (nitrogen source) were found to induce the protease production of the isolate and showed highest proteolytic activity at temperature 35 ° C and pH 6.5.
  S. Sharmin , Md. Towhid Hossain and M.N. Anwar
  Following enrichment media technique a bacterial isolate WP was isolated from degraded pulse and identified as a Bacillus amovivorus den Dooren de Jong. Primary screening was done by gelatin, skimmed milk casein and egg albumin hydrolysis method. Optimum protease producing incubation period, medium pH and temperature for this isolate were 48 h, 8.5 and 37°C, respectively.
  U.M. Seraj , M.I. Hoq , M.N. Anwar and S. Chowdhury
  Attempts were made to isolate, purify and characterize antibacterial peptides from the hemolymph of the American cockroach Periplaneta americana. Both non-induced and induced hemolymphs were tested for their antibacterial activity against several Gram-positive and Gram-negative bacteria. Induction was done by injecting Escherichia coli into the abdominal cavity of the cockroach. A time-induction study showed that antibacterial peptides were induced as early as 1/2 an hour with a peak at 9 h which started to decline around 24 h. The non-induced hemolymph showed activity only against E. coli whereas induced hemolymph showed activity against several Gram-positive and Gram-negative bacteria as well as against one antibiotic resistant E. coli. The induced hemolymph was subjected to SDS-PAGE to estimate the number and molecular weight of proteins present in the crude hemolymph. Seven distinct protein bands were detected by SDS-PAGE. The hemolymph was then subjected to gel filtration chromatography to purify the proteins responsible for the antibacterial activity. Twenty fractions, one ml each, were collected and all the fractions were tested against those bacteria which previously showed sensitivity to the crude hemolymph. Only six fractions were found to be effective against the tested bacteria. The protein concentrations in the active fractions were determined by spectrophotometry. The active fractions were finally subjected to SDS-PAGE to determine the molecular weight of the protein(s) which were responsible for the antibacterial activity. There was one protein in all these six fractions except fraction F13 where an additional protein of 67kDa was present. The approximate molecular weight  of  the  isolated  antibacterial  protein  present  in  all the fractions was 61kDa. It was found that only 2.87 μg of the protein could inhibit bacterial growth whereas approx.10 μg of conventional antibiotics was required to obtain similar result.
  W. Shumi , Md. Towhid Hossain and M.N. Anwar
  In the present study fungal colonies were isolated from different protein sources by enrichment culture technique and the fungal isolates were screened for protease activity. Isolate G, the most potent producers of protease were identified as Aspergillus funiculosus G. Smith. The isolate G was found to show highest protease production (activity) in an optimized medium (Yeast extract 0.5%, lactose 0.1% and KNO3 1%) at pH 7.0, temperature 37°C and 5 days of incubation period. The crude enzyme of the isolate G was found to show its highest activity at pH 7.5 and temperature 35°C in enzyme substrate reaction. The molecular weight of enzyme of the isolate Aspergillus funiculosus (G) was found 51 kDa, which was determined by SDS-PAGE technique.
  Jaripa Begum , H. Sohrab , Md. Yusuf , J.U. Chowdury , M.M. Husain , H.A. Begum and M.N. Anwar
  Azaron or 1, 2, 4-trimethoxy-5-(1-propenyl) Benzene,  isolated form the rhizome extract of Acorus calamus was screened for antifungal activity against three phytopathogenic fungi viz., Macrophomina phaseolina (Maubl) Ash by, Curvularia lunata Wakker Boedijn and Alternaria alternata (Fr.) Kedissler. This compound completely inhibited the growth (100%) of all the tested fungi at 400 μg mL-1.
  M.Z. Alam , M.A. Manchur and M.N. Anwar
  A Streptomyces strain A2 which was provisionally identified as Streptomyces omiyaensis, isolated from goat`s rumen was found capable of producing cellulolytic enzymes during growth on different cellulosic substrates. The isolate A2 grown under different conditions showed that CMC was the best cellulosic substrates for inducing the synthesis of extracellular cellulolytic enzymes. The isolate also showed heavy growth and liquefaction at pH 6.5, temperature 35 to 40°C and 5 days of incubation period during growth in liquid Winsted`s media having 1.2% CMC as a cellulose substrate. Maximum level of CMC-ase (230.56 U mL-1) in liquid media was found to produce when beef extract used as a nitrogen source along with CMC as cellulose source. Maximum level of reducing sugar (473.33 μg mL-1) and protein (324.27 μg mL-1) was obtained when CMC used as a growth substrate. The crude enzyme of the isolate was found to show highest enzyme activity (CMC-ase of 269.44 U mL-1, FP-ase of 64.81 U mL-1, Avicelase of 200 U mL-1 and β-Glucosidase of 138.89 U mL-1) at pH 6.5 and temperature 45°C during enzyme substrate reaction. The molecular weight of enzyme of the isolate Streptomyces omiyaensis was determined by SDS-PAGE technique and found 85 kDa.
  M.A. Kashem , M.A. Manchur , M.S. Rahman and M.N. Anwar
  Two thermophilic cellulolytic bacterial isolates were tested to determine the effect of carbon and nitrogen sources on the production of extra-cellular proteins, reducing sugars and cellulolytic enzymes. Lactose was found to be the most potential carbon source for Avicelase (342.52 U mL-1) and ß-glucosidase (256.89 U mL-1) activity where as NH4Cl was found to be the potential nitrogen source for CMCase (144.68 U mL-1) activity.
  Abul K.M.S. Kabir , Pijush Dutta and M.N. Anwar
  A number of D-mannose derivatives were evaluated for in vitro antibacterial activity against eleven human pathogenic bacteria. These compounds were also screened for in vitro antifungal activity against six plant pathogenic fungi. It was observed from the study that the tested acylated derivatives of D-mannose exhibited moderate to good antibacterial and antifungal activities. The acylated D-mannose derivatives were found to be more effective against fungal phytopathogens than those of the bacterial strains. For comparative study, the biological activity of two standard antibiotics (Ampicillin and Nystatin) were also measured. It was found that the antimicrobial functionality of D-mannose increased with the introduction of various acyl substituents and in some cases, this functionality was greater than or comparable to the antibiotics studied.
  N.C. Shil , A.R.M. Solaiman , M.N. Anwar and M.A. Saleque
  A laboratory experiment was conducted at Bangladesh Rice Research Institute in 2001 to know the phosphorus-releasing pattern of soils in repeated extraction and to find out the effects of organic and inorganic amendments on solution P releasing power of soils under submerged condition. Three composite soil samples (0-15 cm) were collected from the cultivated rice field of Sreepur, Gazipur (Aeric haplaquept) (pH 4.8), BRRI Farm, Gazipur (Vertic haplustept) (pH 6.2) and Benerpota, Satkhira (Haplaquept) (pH 7.5). The soils were different in texture, organic matter and available P content. The soil samples were incubated under submerged condition in plastic pots amending with cowdung (CD) and Poultry Manure (PM) each at the rate of 5 g kg-1, Triple Super Phosphate (TSP) 12.5 mg kg-1 and a control with out any amendment. Taking 3 replications the pots were placed in the laboratory under room temperature (28±2°C) in Completely Randomized Design (CRD) arrangement. At the 3rd week after incubation, the release of solution P (extracted with 0.01 M CaCl2) was determined with repeated extraction for 8 times. In all the soils, the concentration of solution P in poultry manure amended sample was greater than the others. The release of solution P was not much influenced by the soil amendment. The solution P in the first extraction was found highest in all treatments, which gradually decreased in subsequent extraction but from 4th to 8th extractions, the P was found identical. The total amount of solution P released in 8 extractions was found highest with poultry manure amendment yielding 0.24, 0.13 and 0.10 ppm for Satkhira, BRRI and Sreepur soil, respectively. The contribution of cowdung and TSP was almost identical in the tested soils and was lower than poultry manure treated samples. The exponential relationship between solution P and number of extraction hold good for all three soils. The co-efficient of determination (r2) of the exponential equation for BRRI soil was 0.8571 to 0.9404 (p<0.005) followed by Satkhira soil 0.8487 to 0.899 and the lowest in Sreepur soil 0.767 to 0.8781.
  H.M. Cherry , Md. Towhid Hossain and M.N. Anwar
  Aspergillus fumigatus Fresenius was isolated from goat’s rumen and exhibited highest amylase production at temperature 37°, medium pH 7.0 and 3 days of incubation period. Four percent starch as carbon source and 0.25% (NH4)2HPO4 as nitrogen source was found to induce amylase production by the isolate Aspergillus fumigatus. Maximum glucoamylase activity was achieved at 35° temperature and pH 7.0 along with 4% starch as substrate concentration during enzyme-substrate reaction.
  Q. Das , J.U. Chowdhury and M.N. Anwar
  Using synthetic enrichment medium, fifteen bacterial colonies were isolated, purified, preserved and stained for Poly-β-Hydroxybutyric Acid (PHB) granules with Sudan Black B stain. Among them ten showed PHB positive reaction. An attempt was made for the production, isolation and purification of polymer by the proposed isolate QGR. 7.81% of biodegradable polymer production was recorded by QGR. On the basis of cultural, morphological, physiological and biochemical characteristics the isolate QGR was provisionally identified as Listeria murrayi. The chloroform extract of biodegradable polymer of isolate QGR was analyzed by Gas Chromatography Linked to Mass Spectroscopy (GCMS) where it showed eighteen different compounds. Among them, ethyl cyclopropane carboxylate, oleic acid and di-n-octylphthalate are the major compounds.
  S. Sharmin , Md. Towhid Hossain and M.N. Anwar
  A protease-producing bacterium was isolated from rumen of goat and identified as a Lactobacillus species. Optimum protease producing temperature, medium pH and incubation period of this isolate were 37°C, 8.0 and 48 h, respectively. The crude enzyme of Lactobacillus casei showed maximum activity at 50°C temperature and pH 8.0.
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