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Articles by M.M. Syam
Total Records ( 4 ) for M.M. Syam
  Ahmad Bustamam , Siddig Ibrahim , Nirmala Devi , Muhd Nazrul Halkim , Adel S. Al-Zubairi and M.M. Syam
  Cervical cancer is the second most common cancer of female reproductive tracts. In developing countries, cervical carcinoma is the leading cause of cancer fatality in women. Despite attempts to lower the fatality rate, very few in vivo models are in place to investigate this cancer. We therefore are able to develop an in vivo animal model that is suitable to conduct such study. In our attempt to secure an in vivo animal model for cervical cancer, the carcinogenic property of diethylstilboestrol (DES) was exploited to establish a model for Cervical Intraepithelial Neoplasia or carcinoma (CIN). Female Balb/C mice were injected with several dosages of DES (i.p) during pregnancy at day 13-18. Female offspring were reared and sacrificed at age of 48-54 days and the cervix tissues taken for histological evaluation using H and E. The progression of the cancer and hence, disease state is monitored by measuring serum IL-6 using an ELISA kit. Proliferative cell nuclear antigen (PCNA) expressions were studied by implying immunohistochemical techniques. All parameters with regards to CIN were compared to a control group of treating the cancer using a used drug, cisplatin, used preferentially to treat cervical cancer in humans. The results of this study revealed that a significant difference in serum IL-6 concentration between DES-treated group and control groups (p<0.05). CIN histological related lesions was noticed to be prominently dominant in DES-treated animals whilst these lesions were absent in control groups. In addition to that PCNA index in DES-treated animal was found to be a significant different compared to control group. The above findings indicate that DES could be utilized and further exploited as cervical carcinogenesis initiator in animal models to screen and study new potential anti-cervical cancer compounds in vivo.
  S.I.A. Wahab , A.B. Abdul , H.C. Yee1 , A.S. Alzubairi , M.M. Elhassan and M.M. Syam
  The aim of this study is to evaluate some derivatives of Zerumbone for their anti-tumor effects towards human cervical cancer cell lines (HeLa). The MTT tetrazolium salt colorimetric assay was utilized to evaluate the cytotoxic effects of ZER, cisplatin and the derivatives were n-Butylbenzene (compound 5), 1,1`-(4-Chlorobutylidene) bis(4-fluorobenzene) (compound 6), alpha, alpha-Diphenyl-gamma-butyrolactone (compound 7) and (1,4`-Bipiperidine)-4`-carboxaminde (compound 8). The results of this study showed that derivatives of ZER have shown lesser anti-tumor effects towards HeLa cancer cells compared to the principal compound (ZER).
  A.B.H. Abdul , A.S. Al-Zubairi , N.D. Tailan , S.I.A. Wahab , Z.N.M. Zain , S. Ruslay and M.M. Syam
  A natural compound, zerumbone was extracted, isolated and purified from the rhizomes of edible plant Zingiber zerumbet using methanol extraction and Column Chromatography (CC) method. The isolated and purified zerumbone crystals were subjected to High Performance Liquid Chromatography (HPLC), Liquid Chromatography Mass Spectrometry (LCMS) and 13C NMR and 1H NMR analysis to confirm the purity, molecular weight and molecular structure. The study investigated the purified zerumbone crystals for its anti-cancer properties on human cervical cancer cell line (HeLa). Cisplatin, was used as a positive control in this study. The cytotoxicity of zerumbone and cisplatin were investigated using the MTT assay and caspases-3 was estimated with colorimetric assay in zerumbone treated HeLa cells. Morphological analysis showed that there were changes observed on HeLa cancer cells after treatment with zerumbone and cisplatin. The MTT assay results demonstrated that the IC50 value ( ± SEM) of zerumbone was determined to be 11.3 μM (2.5 μg mL-1) whilst the IC50 value of cisplatin was at 7.5 μM (1.6 μg mL-1). Prominent growth retardation was identified to the HeLa cancer cells, after treatment with both compounds, while caspase-3 was observed to be significantly increased in zerumbone treated cells as compared to untreated control cells. This study showed promising avenues towards zerumbone to be developed as a new chemo-natural drug for treatment of cervical cancer.
  Mohamed Yousif Ibrahim , Ahmad Bustamam Abdul , Siddig Ibrahim Abdel Wahab , Manal Mohammed Elhassan , Adel Sharaf Alzubairi and M.M. Syam
  Zerumbone is a natural compound isolated from the fresh rhizomes of Zingiber zerumbet. This bioactive compound has shown a chemo-preventive, anti-inflammatory and free radical scavenging activities. This study examines, the effect of zerumbone on the extent of tissue damage in Cisplatin-induced hepatotoxicity in rats. The rats received a single dose injection of 45 mg kg-1 Cisplatin. Other groups of rats received zerumbone (100 and 200 mg kg-1), corn oil or the vehicle (DMSO) intraperitoneally for 4 days prior to Cisplatin injections. All animals were decapitated 16 h after Cisplatin injection. Trunk blood was collected and analyzed for alanine aminotransferase, aspartate aminotransferase, lactate dehydrogenase, alkaline phosphatase and gama-glutamyl transferase. Liver tissue was kept for the quantification of malondialdehyde and glutathione levels. Histopathological investigations were carried out and severity of lesions was scored to obtain quantitative data. This study revealed that zerumbone reduced the extent of liver damage and preserved liver functions as proved by microscopic observations and lesion scoring. Increase in liver MDA levels with a simultaneous reduction in GSH in the Cisplatin 45 mg kg-1 group was attenuated by zerumbone treatment (p<0.05). Zerumbone is beneficial in Cisplatin-induced liver dysfunction and organ damage in rats via prevention of lipid peroxidation and preservation of antioxidant glutathione.
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