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Articles by M.M. Mazen
Total Records ( 2 ) for M.M. Mazen
  S.R.E. Abo-Hegazy , Noha F. El-Badawy , M.M. Mazen and H. Abd El-Menem
  Thirteen faba bean (Vicia faba L.) genotypes were assessed under greenhouse and field conditions in 2008/2009 and 2009/2010 seasons, using morphological and molecular characterization methods for resistance to chocolate spot diseases caused by Botrytis fabae A significant variation among the tested genotypes and their reaction to the disease was found under greenhouse condition. Sakha 1, 2 and Nubaria 1 were resistant, indicating by the lowest disease severity. Whereas, Giza 40, line 375 and Cairo 25 where susceptible. Other faba bean genotypes were moderately susceptible. RT-PCR analysis using chit 1 and 2 primers amplified fragment of chitinase gene (900 bp) in six faba bean genotype upon infection with the pathogen 48 h after inoculation. This fragment detected only in the resistance cultivars Sakha 1, 2 and Nubaria 1 and some moderate resistance genotype including Giza 3, line 24 H and line 36. At the same time no signal was detected in other infected genotypes or healthy ones. Field results of testing genotype against chocolate spot diseases often do not correspond completely with greenhouse results since the environment is less controlled and possibly natural infection could introduced unwanted pathogen(s) or other factors. The estimates of heritability in broad sense ranged from 0.90-0.99, high values of heritability were obtained for seed yield/plant (0.99), followed by plant dry weight, plant height, seeds pod-1 across the two seasons. However, No relationship was found between resistance of genotypes against B. fabae and their morphological characterizes under field conditions.
  Muneera D.F. Alkahtani , M.M. Mazen , M.A. El- Naggar and M.K. Arfa
  Mycotoxins is a serious problem threatening human and animal health and detection is the most important steps to get rid of them. The impact of these mycotoxins on seed physiological characteristics as discoloration is important things that are related with seed quality as well as marketing. The fungal isolation was conducted from three cultivars of bean seeds (Giza3, Giza4 and Giza6) after dividing into groups depending on their color. Sixteen fungal species were associated with discolored bean seed samples. The Aflatoxin (AF), Alternariol (AOH) and Zearalenone (ZON) were determined using immunoaffinity columns and GC-MS. Aflatoxin was existed in all colored seeds while, the dark brown colored seeds contaminated with AF, AOH and ZON. The highest concentration was found in dark brown colored seeds followed by golden brown. However, Zearalenone (ZON) was detected only in the dark brown in high concentration (45 μg kg-1 seeds). AOH toxin excreted by both isolates of A. alternata ranged from 1.0 to 3.0 μg g-1. The highest level of AOH toxin has been detected after 14 days of incubation and then decreased. Whereas, the normal seeds contained the lowest concentration of Aflatoxin. The amount of Aflatoxin produced by A. parasiticus increased positively with increasing incubation time. The amount reached 200 (μg kg-1) after 35 days incubation. Nevertheless, this amount was decreased to 125 (μg kg-1) after some period when bean seeds simultaneously inoculated with A. alternata and A. parasiticus. In conclusion mycotoxins affect on the seed physiology, there is a direct relationship between the rate of mycotoxins (AF, AOH and ZON) contamination and the degree of discoloration. The presence of more than fungus together may result in lack of mycotoxins content.
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